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CRIMIC CR01435

Gene CG30008

CG30008

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9890212..9890232	46C2	FBti0210372	86385

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACTTGACATTAGCAATTCAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTCTTGGCAAGCAAAGCG	TCCACTGACCCACATGCAAA	CGGGTTCGCTGGCTTACTTA	GTCACGGGAGCTGGATCAAA

 

Amplicon 5'

Amplicon 3'

AACTCTTGGCAAGCAAAGCGGATAGGCCATCATGCTTGCTTTATATCTTTTGGGACGAAAACGCGAATTGCAAAAAGAAACGTGCTGCCCTCTTGATCTACATAGTTTTTGCTTACGTATACCACTAGACGTCATAAAAAATCAATGCAAGTGTGGAATTTAAAATGAGTTGGTTCTTCAGTTCATAAAACAAACAACGTCGTACTTAAACTATTACGGTGAATTATGAACTATTAGTGAAACTGCAAAACATTGATCAAGCTTTCCTTAGACAATCTTTATTTCCATTTACTAAGGAGACACTTACCTGAAAGTGGAAGGTCTTATGGTCCACAGTGATTGTGAATGTGTTGTCCTCCTGGTCGTCGATTCCGATTAGCGCATCCTTTAAACGAACACAACCGCGCCTCACGCCCTTTATCATCTTATCCTTCGACTGTAACAGAAATGCAGTCGGAATTAGGAAAGTTCATTAAATCGCAGCTCATACTGTCAAAGGGCTATTACGCCTGCTATAAATTAATGTATTCTAATCAATGCTTGGGGAATATAAAAACAGTGTTCGGCGTGCTGTTCACGCTTAAATGTTCGCCAGCGGTCAAGGCGGTAGAGCTGCAGTCCACCAGTCCCACGCCACCTAAAGGATACAGGCCAGCGGAAGCTCAACACAAAGAAGCGAAATGGAGGCGTCAGCAAGTGAGCTCCTCTTGGATTTGTCAAAAAACTGTCTGATGGTTTCAATAAAATTGTAGGTATAAATTTGCAGCCGGTGGTGAACGTTCCTACGATTTATAAGGATCCCTGGTACATGATCACAGTGCTGGTCCTCTATCTGTACTTCGTCACCAAAGCGGGTCCGCAGTAAGTTCACATGCTAATGTCAGCGTCGCTTGTTTGCATGTGGGTCAGTGGA

CGGGTTCGCTGGCTTACTTATATAAGAAAGAAGACCTTTAATACCTCGCTTATAGTTTCATGGAATGGCGCAAACCTTACGAGCTCAAGCGACTTATCTTGCTACATAATTTCATCCAGGTGGTTTCCTGCATATATGCTATCAAAGAGGTGGATATTTATAAATTGTACAGCAGCAGCAGCATCGGCTAATTGCTTTTCTCTCGCAGGTCCTGTACATTACGGACAACACCATATACATCTTTTGGAAGTGCCGCGACATCGGGAGCAGCCCAGAGCTCGTGAGGCGGTACTACAACTTGGCCTACTTTCTCTTCTGGCTGAAGATCTCTGAGCTGATTGAAACCGTGATCTTTGTGCTTCGCAAGAAACAAAACCAGGTGTCCAAGCTGCACATCTTTCATCACTTCTCCACGGTAACGCTGGTCTACGCACTGATCAACTTCAACGAAAATGGTAAGCTAGCCGGCTGCTGGGTGACCGACTATTTTCATAAATGCGTTCAATTGTACAGGTTCTGCTGCTTACTTCTGCGTGTTCTTGAATTCTATCGTTCACGTTATCATGTACTCGTACTACTTTGTGGCGGCAGTGGCGGATAAGACCTTGGTGCAGGCCCTAACTCCAGTGAAGAAGTGCATCACAGTGATTCAGATGACGCAGTTCGTTCTTATCCTCACGCAGGTTGCTTTCCAGCTGGTATTGTGCGGCATGCCGCCCTTAGTGCTTCTCTACTTCACCACCGTCATTCTTGGCATGTTCTACGGCTTTTACGACTTCTATAATAGTGCCTATCAAGCCTCGCAGCGGCGCAAGAGTCAGACCCCTCAGTCAGACTCAAAAAAGTAATCTCCTGTCAACAAATCACGCAATAAAGGGGTACCAACAAGTGTGTATGTGTATCCAAATGTATCTATAGCAGATAAGATAAGGCTGCGGGCCATGAGGAGTTCGTTCTGGGTGCGATATGGGTTTCAGTCAGGAGGTGCACTGCAGCCACTCACCGTGTAATAGGACAGCAGTCCAGCATTCTCGTCCAGAACGAAGAAGCGGTACTGCCAGCCCTTCATCACATTCGTCCACTTGCTTAGCGTGCCCTCCAATGTGCCAGAGCCACCACTCCCGCTCTGGGGGGGTCCCAGGCTGGGGGCACTTCCAGTGGTGCTGGCCATTTGATCCAGCTCCCGTGAC

 

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