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CRIMIC CR01436

Gene CG5726

CG5726

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:18179298..18179309	55B12	FBti0210373	86386

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AACGAAACACAGCTGACTGATGGCGA	TTGGTGTACATTGTCTCCAGGTCGTG

Images

Construct Design Sequences

sgRNA sequence	ATGGCTGGCTTTGATGGGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCGCATGATCTCCCCATT	CATCAAAGCCAGCCATGTGA	CTCGCCTTGCCCATATGCTA	ACTTTAGCCCCATGGTGGTC

 

Amplicon 5'

Amplicon 3'

TTGCGCATGATCTCCCCATTCAGTGTGATCTGTGACAGCACCAGGCGGGCAATCCGAGTGCCCAGCACCACCGACGTGTCATTGAGCTCGCGGGTAAGCAAGTCCAGCAGGGACTCACCCAAAATGGTGATCGGGGTATTATCCGCCAGCCTTACCCTGTGATAATATTCACCCAGCAGCGTGATCGAGTTGTACAGTCGGTTCTTGTCGTGCAACCGATATGCGTCCGCGTTGAGGAAGTTTGTCTCCAAAATCTTGAGCATGGCGCTGCGCACCTTCGTCTCTTTCATGGCCAGTGCGTCGAAATTTCTCGATGAGAAGAGCAGGGCGAACTTTAGCGCCGTATCACCATCGCTAAGCGCCTTGTCGTTCAGGTACTCCATTGACTCACTGAAAATTGCAAATACAATTAGTTTATTTACTTTTCATTTATTAGTATGTTATAATAATCAAGTCTACTACTACTTCATAATGTTAAAGTTTGCACTACAACTGTGTTTAACACCCCCACTGATATAACGCATAGCAGCATTGTTTGATAAGCCCAAGCTGCGAACTATTTGTTCAGCAATTAACTCGAGCATGAGCCTGAGAGAATCGGAGGGAAAAATGTTCACTTTGAAATGATTCGATGGCGCCGATGTCTCTCTGCCCGTCTTTATTTGTATGAAAAGTTGTAAATTTTCAAGACACGCTGACGTTCGAGCAACCTGGTCGCAAACAACGAACTCGTGCACGAACACATATACACAAATACAGTGCTTTTCAGATTTATAAATCGATTTTTAGACATTTAATAAAATTGAAATGTATATGTAGAAGGTCGCAAAAACTTTTTCGAACCTAAAAATAATGAAGTAAGAAGAAAAGTTTACAAAGTTGCACTTTAAGAACCCAAGGGTTCTTGAACGCGTAAAAACAAATTAACGTTTGAACTTGATTAGTAATTCGGTTTCTGCATTTCAAGCATTTCATTATTGGAATAACTTATAGAATGCTGTAACCTATGGGCGAACGCCACAAGCTCCCAAATTTAACGAATTACTCATGCTACTTGTAATGGAATGTCATAAATATTACAATAAGGATCGGTCTTCGCCAGGCAGATATGTACTGCATGCATTAAGTCTTGTTATCACATGGCTGGCTTTGATG

CTCGCCTTGCCCATATGCTATCGCACCGCACTGTACCTACGCCGGGTATTTACATACATCCATATGTATATGCGAAAAGAGGTGGCAAATAAACACACTACTTATGATATGATTAATCATCAATGAGCGAACCAACCAAACGGTAGAGATCGGCACACATACCAAAGCATAGACTCGTCGCGCACTATGTTGCAGAAGTCCGTTTCGATGGTCTTGATCTTGGAGTTCAGCACAATGCCATCGTCCTGCAGATCAAGGTTCCTCATCAGGGCGATGATGTGGTCGTAGGCATAGCCACCTGTTTGATTTTGATAGGCGGCGGGGCCAGTGTTCAGGATGGTACAAACATCCCGGAGGCGCGCTCCCGTTCCCTCACCACCGCCCCCGCTACCGACACCACTGTGCGAACTGTGACCACTGTCCACCGCACTGTTGTTGTAGCTGTTGTTGCCGTAGGAGGTGGTGTTGCTGCTGCCACCGCCGCCACTGTTGCTGCTGCTGACCATGTTGTTGGCCATTTCCATTTCTCGCCGCGATCACTGCCGTTTGTTGTTGTTATCGCACGGTTGTTTGTCTGTCCCTTCCACACACACTTCAGTGTCTGTTATATTGGTACGCGCGTGTGTGTGAGTCGTCGGTCCACCAAATAAGAATCAGCGTTGACAAAGGTGTCGAAATGCGCACGCCAAATGAAACACCTTTTCGCTCAGCTTCCGCTTTGGGTGCAGGTGCAGTTAGCTCACTGGGACCCACGGTCGATTGAAACGCAAATAACGAACGAAATTAAATGCACAAATTTATAACTATTCCTATTTATTATTGCTATTTGACGACTTTTAAAAGAGGTGGAAAAACATCGATGTGTTGTGTTCTTTAAAACATCGATGTTTCTCCACCTCTAGATAGCATTGTCGCGATATCGAAAGCACACGGTGTGCTTTCGTAAATAGCGCCACAGTGCTCCCGCTGTTATCGATAAGACCACCATGGGGCTAAAGT

 

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