CR01436

CRIMIC CR01436

Gene CG5726

CG5726

Information

Donor Location Cytology FBti Stock number
pM37-p22R:18179298..1817930955B12FBti021037386386

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK long band

Images

Construct Design Sequences

sgRNA sequence ATGGCTGGCTTTGATGGGCTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTGCGCATGATCTCCCCATT CATCAAAGCCAGCCATGTGA CTCGCCTTGCCCATATGCTA ACTTTAGCCCCATGGTGGTC
 
Amplicon 5' Amplicon 3'
TTGCGCATGATCTCCCCATTCAGTGTGATCTGTGACAGCACCAGGCGGGCAATCCGAGTGCCCAGCACCACCGACGTGTCATTGAGCTCGCGGGTAAGCAAGTCCAGCAGGGACTCACCCAAAATGGTGATCGGGGTATTATCCGCCAGCCTTACCCTGTGATAATATTCACCCAGCAGCGTGATCGAGTTGTACAGTCGGTTCTTGTCGTGCAACCGATATGCGTCCGCGTTGAGGAAGTTTGTCTCCAAAATCTTGAGCATGGCGCTGCGCACCTTCGTCTCTTTCATGGCCAGTGCGTCGAAATTTCTCGATGAGAAGAGCAGGGCGAACTTTAGCGCCGTATCACCATCGCTAAGCGCCTTGTCGTTCAGGTACTCCATTGACTCACTGAAAATTGCAAATACAATTAGTTTATTTACTTTTCATTTATTAGTATGTTATAATAATCAAGTCTACTACTACTTCATAATGTTAAAGTTTGCACTACAACTGTGTTTAACACCCCCACTGATATAACGCATAGCAGCATTGTTTGATAAGCCCAAGCTGCGAACTATTTGTTCAGCAATTAACTCGAGCATGAGCCTGAGAGAATCGGAGGGAAAAATGTTCACTTTGAAATGATTCGATGGCGCCGATGTCTCTCTGCCCGTCTTTATTTGTATGAAAAGTTGTAAATTTTCAAGACACGCTGACGTTCGAGCAACCTGGTCGCAAACAACGAACTCGTGCACGAACACATATACACAAATACAGTGCTTTTCAGATTTATAAATCGATTTTTAGACATTTAATAAAATTGAAATGTATATGTAGAAGGTCGCAAAAACTTTTTCGAACCTAAAAATAATGAAGTAAGAAGAAAAGTTTACAAAGTTGCACTTTAAGAACCCAAGGGTTCTTGAACGCGTAAAAACAAATTAACGTTTGAACTTGATTAGTAATTCGGTTTCTGCATTTCAAGCATTTCATTATTGGAATAACTTATAGAATGCTGTAACCTATGGGCGAACGCCACAAGCTCCCAAATTTAACGAATTACTCATGCTACTTGTAATGGAATGTCATAAATATTACAATAAGGATCGGTCTTCGCCAGGCAGATATGTACTGCATGCATTAAGTCTTGTTATCACATGGCTGGCTTTGATG CTCGCCTTGCCCATATGCTATCGCACCGCACTGTACCTACGCCGGGTATTTACATACATCCATATGTATATGCGAAAAGAGGTGGCAAATAAACACACTACTTATGATATGATTAATCATCAATGAGCGAACCAACCAAACGGTAGAGATCGGCACACATACCAAAGCATAGACTCGTCGCGCACTATGTTGCAGAAGTCCGTTTCGATGGTCTTGATCTTGGAGTTCAGCACAATGCCATCGTCCTGCAGATCAAGGTTCCTCATCAGGGCGATGATGTGGTCGTAGGCATAGCCACCTGTTTGATTTTGATAGGCGGCGGGGCCAGTGTTCAGGATGGTACAAACATCCCGGAGGCGCGCTCCCGTTCCCTCACCACCGCCCCCGCTACCGACACCACTGTGCGAACTGTGACCACTGTCCACCGCACTGTTGTTGTAGCTGTTGTTGCCGTAGGAGGTGGTGTTGCTGCTGCCACCGCCGCCACTGTTGCTGCTGCTGACCATGTTGTTGGCCATTTCCATTTCTCGCCGCGATCACTGCCGTTTGTTGTTGTTATCGCACGGTTGTTTGTCTGTCCCTTCCACACACACTTCAGTGTCTGTTATATTGGTACGCGCGTGTGTGTGAGTCGTCGGTCCACCAAATAAGAATCAGCGTTGACAAAGGTGTCGAAATGCGCACGCCAAATGAAACACCTTTTCGCTCAGCTTCCGCTTTGGGTGCAGGTGCAGTTAGCTCACTGGGACCCACGGTCGATTGAAACGCAAATAACGAACGAAATTAAATGCACAAATTTATAACTATTCCTATTTATTATTGCTATTTGACGACTTTTAAAAGAGGTGGAAAAACATCGATGTGTTGTGTTCTTTAAAACATCGATGTTTCTCCACCTCTAGATAGCATTGTCGCGATATCGAAAGCACACGGTGTGCTTTCGTAAATAGCGCCACAGTGCTCCCGCTGTTATCGATAAGACCACCATGGGGCTAAAGT
 

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