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CRIMIC CR01440

Gene CG5335

CG5335

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:18621927..18621948	55E2	FBti0210375	86389

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	GACTAATTACTTGTCAAATTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTCGCTATCGGACATGTCA	GAAAACGGAAAAGCGGTCTT	TGGGCTTCTTATCAGTACGTGA	CGGCCCCTGGAAATGTAAGT

 

Amplicon 5'

Amplicon 3'

CGTCGCTATCGGACATGTCAAATTCTTCATCATCAAAATCGATAATCTGTGCAAAAATATGAATAATTTTTAAATATCTCCATTAGATTAAACACAGGAAACAAACAAACCTCGCTGTTTAACCGCTTGAATTCGGAGATTCCCGTCAAATCCTCCAGAAACTTGGCTGTTTCAAAAGTTTTAAACAGGAAAGCATGACCCTCTTTTTTATATTCATTTAGGACGGCCTGTAAGCGAATATATTAAAAAAAAAATTTTAACAATATTGAAAACGCTATCAAAACCATTTTAAAAGATTGTCAAATCTTACCGCTGAACAGGCTATGCACTGAGCAAACTTTTGAGTGGCGGGCAGAATATTCTCGTAGTTGCACAGCATTCCACGGATGGAGTGAGGCAAAATTCCTAGCAGACCCTCCGGCACCTTTTCTTCCGTCTCCTCCGACCTGCCACGCCCACTCTGTGCATAGTAGGCGGGTGCCAGCTCCTTACGAGGATGCTGCAGCAAAGCCACCAACAATTCCACCGCATAGCTGGCCGCAATGTTGGATACTCCGGGTCGTGTCACTGTGCACTGTTGATCCAAAGTTCGGTCTTTGAGCGACTGAAAATGGAATAGAACGAAATGCAATGAGTATCTGTATCCAAATTTGTACGTACAACTTTATAAAGCGAGTCTATAAGAGATTTTTTTATACAGAGTTTTTTTCAGTGTGATTTGGAGCACATTCGACGCCGGTGCAGTAGTACGATCAAGAGCTTTTGAAAGCTTTTCTGTGTCCACAAACACATGCTCCGAGCAAACAAAGATGACGCCTTCGGGCGAGAACAAAACAAATAGCAGCGCTTAAAAACCAAAAACACAAGATATAGAAAACTTCGGCGTTTGTATCGGTTACTTGAGCAACCCGCCGCTCCGCCCATCACTGATTAGATAAAGTTTTAACATTTGGGAGAGCCGGAGAACTCCGGACTCTCCCACGGAACTATAAAACACATTTTGCGCTTGATTGTTCAGTACAAAGGCGATAGTTAGTTGAATTGGTAATTGCAGGAAGGAATCATGTTGACCGAATTCGCTGGCAATCTATCGCATCCGCTGGAGTTTGGTCATGTTCTGGAGGTCGTGGCCAAGACCATCGATGGAGCAGCCAGGTAAGTGTTGTCCGGCATTAATGTAAGACCGCTTTTCCGTTTTC

TGGGCTTCTTATCAGTACGTGACTAATCGATTGTGATCATTTGGTTCGGATAGGTTCCACATTAACCTCTGCACGGCCAAGAGCACGGTCGATCCGAACGCAGATATTGGGCTACGCTTCTCATGCTATTTTCGGAACGATGTGATCGTTCGAAATTCCCGGATAAACGGCGCCTGGGGCGAGGAGGAGTCCCATGTCATGGACCCCAACACCCTGCCCAATCCCATCGTCAGTGGCGAGTTCTTTTTGGTCTACATTCTTTGTTGCGAGGACAGCTTTGCCATCTCGATAAATAGCCGAGAGTTCTGCAGATTCCGCTACCGCATGCCGTTGGGAACGATTCGTGCTCTGGAGATCCGTGATCAGATCCAAGTCATTAAACAGGTGGATCATCGTACTGTGTTTCCAAATCCGTGGCCAGCCGTTCACGCCTCGGACTACTTTAAGGCGTTCAGTAACGATCAGCCAATTTTGTTTAGTCCGGGCCATGTGATTGTCCTTACAGCTCGGTGTTTCGAGAATAAAAAGGGACAGTTCATAATCAAGTTCATGGACTCGGATACCAAGCGCGAAGAGCTACACTTCAGTGTGCGTTTCGATGAGAAGGCAGTAGTGCGCAATTCCATGAACAAAAATTTTGAGTAAGTTAACTTTATAATGCCGTACCTCTTTGGGCGTTAACTAATTCTTTTTATAGATTCGGAAGCGAGGAGCGTCATGGAGGTTTCCCCTTCGTCTTTAACCAGCAATTCAAGCTGGCGCTTGCGTTTACGGAACGCGAGGTTTTGACTGCAGTGGACGGTTATAACTTCTTCAGCTACACCTGGCGCACACCTAATGCCATGATGAATCTAGTGGGTTTCAAGGTGACCAGCATAAATGGCCTGGTAGTGCAGATAACTGGTGTGGATCACCTGCAGACGGGGGATCCTACGTGCGCTGGCTTCGAAAAATATTCCCGCCATGATTACGAATGCGTTTAGTAACCATGTTAACCCAACTAATGAAGTCTGAAAATACAAATGTTATCTTAAACTATACAACAAATGGGACTTACATTTCCAGGGGCCG

 

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