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CRIMIC CR01441

Gene CG1441

CG1441

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9900927..9900947	46C4	FBti0210376	86390

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGGATGCTATAGTAATCGCGGCACA	AAAGAGAGCAGGCATCCACTGGAAGA

Images

Construct Design Sequences

sgRNA sequence	GGGATTAATGGGGCCACGTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCAGTACATGGTGCGGAT	GTGGCCCCATTAATCCCCAT	TCCACCTGCCACCCACTC	TGTTAAGCGGGTCCAAGTCC

 

Amplicon 5'

Amplicon 3'

TTGCAGTACATGGTGCGGATGACACCCTTTCCGGCTCCGATAAGCAGGCCGGTGGGTCCGTTGATGTTGTCCGTCCAGCCGGGAATGGGCTCCTTCCAGATGGGTATGACGATCGAGGGGCGCAGGATTACGGCGGGAAGCTCTTCGAACTTCTCCACCACCAGCGCCTCAGCCAGTGACTTGGTGTAGGCATACGTGTTTGGGATAGCCCCGAGCACCTTGCGCTCGATGGTGGCCACCTCGTCGTCCGTCAGCCACTCGCATGCCTGCATCACCTTGTGCGGATCTGCTGGTGGATCGTAGGGCTTCTCGTACAGCGTCTTAACGTGCAGGTGGCAGTACGCCGTCGAGCAGTAGGCGAAGAAGTCCAGGTGCTTCAACGTCAGAGCCAGCTCCAGCATGTACTTGGTGCCGCGGGTGTTCATGAACACGGCATTTCGTAGGGGTTCATCAAAGCTGGCCAAGCAAATGGTATAAAAGTGCATAATTAGAGGGATGTACCTATAGACTTAACATTAAGTAGATGGTTTTAAGGCCATAAGATAGGGAACGGATGAGTATGCATAGATACTTACCGCACTGTAGCTGCACAATGGTACACAATGGACACCTCCTGGCGCAGAGTTTCCAGATCTTTCTCGGAGATTCCCAGGCCTGGCGAAAGGACGTCGCCGGCAATGGCCACCACTTGCTGCAGGATGTGCTCCTCGCCACGCATCTGCTTCACTTGGTCGAAAAGCTACACATACACCACACACCACACGCCACAAGTAATTTGATTAATTTCGTAAGCCAGGCAATGAATTCTTATAGTTTCCATAATTCCCACCCTGCTTACCACATTCTGGAATATATCCTTGATGCGCTCCTGAGGATCCTTGCCCTTCTTAGGGCGGATGAGCAGATAAATGCGCTTTAGGCCACCGCACGAACTGCACGAATTAATAATATTGCATAAGCCATCGCGGAAATGGGGATTAATGGGGCCAC

TCCACCTGCCACCCACTCCACCCACTTACCGCAGCAACTTCTCGACCAGGACCTTGCCCAGGAACCCAGTGCCTCCTGTGATGAACAGACTGCGACCCTTGAAGCACTCCGCAATGCGGTCCACCTCGTTGTCGTCTATCTCCCCAATGGTGCCCCGGTCATCCTTCTCATACACAAACTTCGGCTTGGCGGTCATCTCAAATAGCTTCTGTCACACATAGAATCAAATCATTGCATTAAGTCATTTTGTTATGGTTGAGTATTTTGGGTACACACATTTAATTTTCTTGGGAACTATCATTTTAAACTATAGATTTTCGATATAGATGCAAAGATAGAATTCCCCTTCCTTTCAGATTGAATTTAATATCTTGTAACTACCACTGGCTTTAATACGTATGCCTATCAGAAAATATGATCTCTAAAATGGAGTTGACATTGTTGGGTCTTTTTGGACACTTATGTATGTATGGGGCTGCGACCACGTAATGGCCGTCGCCGCCAAGTGCACTTTTAATTTCATTTGATCTTGGCTGTAAAGTGGTCGTAGCCACATGTGAGTACGAGTATGAGGCAACCAACCAGTTAGCCGGCGGAGCGACAATTGCAGATTAGGCCACGTTTGGCTGACTGGCTGGACAGTGGCCACATAACTGGCTGGCTGGGTTTCTGAGTGTCAACGCAGTGACCATCGAGTGGGCAAGCGGAGCGACACCGGATTGGCGTTACGGCACTAATTAAGCTATTGCCACATCCCCACGTCCACATCCGAGCCTATGTCCATGTCGCATGCCCCACGATTCGCACCATGAGCTTGCCATGGGCGGTGGAGCATGCCGGGAGCACCTCGTGTCCTTCATCAACTCCACTCCGATGACGTAAACTTGATCGAAAGCCGTTGGTCCGCTGACACATTGACCCGCATTCGACGCACACTTGGTTATTAGACGGCGCGGACTTGGACCCGCTTAACA

 

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