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CRIMIC CR01442

Gene ReepA

CG42678

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:22926561..22926584	59B6	FBti0210377	86391

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GTCTCTGCTCGGCGTTCTAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTTTCAAGCTCGGCCTTCG	CAGAGACTGCCGTAGCTCG	ACTATCTGCGATCCGTGCTG	AGTTCTCTGGTCCCACCTGT

 

Amplicon 5'

Amplicon 3'

CTTTTCAAGCTCGGCCTTCGAAAGCGAGAAAAGCACCTACGAAGCCCGTCGTCGTTTCCTAATTGTGTGGGCGTCACGCTTCTGATTTCTAAGTTTTATACATTTCCGTGGTTCCTCGATTCGCTGATTGCCGGATTGTTTGTTTATTAATCAGCTGTCGCAAAGTACCGTTCACTTGTGCGCGAGTGTGCGCGGCCTGTGTGTGTAACCCTCGATTGAACTCCTAATTTGGAGTTGCAACTTTCCAAGCTTCTGCGTCTCTGTGTTCCCGAGGCCGACGCACTCGCCTCGCCCGATTTGGGTGCAGAACATGTGGATATAGCCTGTCGGCGACCCGGCGACTCTCTGTGACTCCGCTATTGTTGTCAATATTTATAGATCGCAACTCGACGCTTGCCCATAGTGTGGCACAGTCGCGGCCAAGTAGCTAGCACCTCATTTGTTCAAGCACACAATACCTCCGTTAGCATTTTCCATTATGTCAATACATCTTAACTTCGAACTAGACTTTACTTTTGATAATGAAATTAGTAATCGGTTCAAATCAATAACTAACATCATAGTCAAGATATATAGTTCTTACATCTAAGGAATTAAATCGAGGCGATTTCTCCCATTACTCGCTAAAGTATCCTTTCCAAATTAAAGATTCAAATATCTTGATACTGAGGTGCTATTTTCTTGAACGCCTCTGTCGCAACATTCGGAACTGCGGCGCATTCCTACGTGTAAACATTGAGTGCTCCTCTGTTGCTGGTTCTCTCTAGACCCGATCGTGGGTTTATTAATAGACCTCTCGCCTGAGCGTTAGACGCCGAGCTACGGCAGTCTCTG

ACTATCTGCGATCCGTGCTGCGGGCAATGCATCCCCGTTGCCATGAGGCCAATCTATCAGCAGGAGCCGCCGCTCTTCTACCAGCGGCCAGCTAATCTCCGCCGCCTACGTCATCCGCCGGGCACCGTCTACGGACGCAGTTACTCGCTCGAGGACCAGCCAGAGGACGTGATACCCGTGTATCCAAAGTTTATCTACGCCCCACAACAGCAGCAACAGCAACAACGGGCCCCATATCCGCTGTATCAACTGGGCGACTCGCTGAGCTCGCTGGACAGCGATTTTCCCGAGAATGAGTACGCGGGTGCCTACATAGTGGAGGATCGAGTTCCGCTGGCCAACGCACCTACTCCCAAAGTTGTCCAGAACCTCAATGCGTTGGGTCGAATGCTGGAGCTGCTGCAGCGCTGTCCTCTGCCATGCCTCTCCTTCAACACGGCCTGCATCTGCTCACTGATTGTCATATTCCTTGCGCCTCGCACTTGTGCCCAGAGTTTACTGTTTCCCGCTTTCAGATTGTTCTGCGGCACCCTGTACCCGGCATATGCCTCATACAAGGCCGTCAGGACCAAGGATGTCAAGGAATATGTAAGTATCTGAACACGTAACTAAAAACCGTAGCGAACTGAGCTAAGGTTTATAATGTTCATTCATCTCTCGCCTCAGGTTAAATGGATGATGTACTGGATTGTCTTTGCATTTTTCACCTGCATAGAAACATTCACGGACATATTTATCTCCTGGCTACCGTTCTACTACGAGGTGAAGGTGGCCCTGGTGTTCTGGCTCCTCTCGCCGGCCACAAAGGGCAGTTCGACTTTGTATCGCAAATTCGTGCATCCTATGTTGACGCGCCACGAACAGGTGGGACCAGAGAACT

 

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