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CRIMIC CR01444

Gene CG17385

CG17385

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:14410655..14410659	51A2	FBti0210378	86392

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCGAATAGGCAACTTAAGGTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCAAGGACTGTGCGATCAA	AGTTGCCTATTCGACACCGA	GGTAGGTCTGAACAGGTTGAAC	ACAAGTCACCGTTCCCAACA

 

Amplicon 5'

Amplicon 3'

AGCAAGGACTGTGCGATCAATTATTGCTCCATGACTGCTGGCGGGTAAACAAGTCAGGAACGTGCCTTTAATTAGTTTCAATCAAATAGTCGAGAAATCACATGTATAAATTATAGACCCATCGGGCGATTCCTTTACCTTTCTTCTTCGAACTTAACGAACAACAGTGAGTGGTAACGTGACTATTTGGGCAAACATATTTGTCACATTAATGACGCAAGAATGTTGGCCTGACAAGCTGGAAAAGGGACAGTTTTACGGCTGTGCGAAATGTGTCATTAAAGCGACAATTTACGCACTGTGAGGTTTTGGGACTTGCGAACGAGAGAATTGATGATGTGACTCGGGAAAATCCAGCGAATATCATACATATGCTCATAAAATGGCACATCATAAAGGGTGCAGTTAAAACGTCTTTAGGGTCCGTAAATCGACGGGTTAGCCTGATTCACGCAACAATGTCAACAGAGATCGGATCGATTAGCAACAGGGATAGCGAAGGATTCGCATTGGTTATCTGCATATTTATTGCATTTATTGGTGTAATTCAATGGAGAACTATGTTGGCCTGCATATATATTCATATCTGTATATTCAATGCAATATTCTTCACATTCCATCTTTCCGTCGGAATCGCCGCTATTTGTATGTTTAGGTGTGAGTTGGGTTGTGTGTTTTCGTGGTATTTCATTTAAAATTATTGCCATTTCTAATGTGTAGTCTCAATTATGATTTCGTGCGTATCCAGATCGGGCACGGCAAACTTCATGGGCAAATAGTCGTCTGGATGCATGTGGGACATCTGGTTAACCTCCAGCTGGGCCCTTTCGTGGTCCTCATGGCACTTGGCTTCGTGCTTCTCATAGTCAGCGAAGGTATCAAAGATGGCCCGACACACCATGCATTCGAAAAAGCTTCTGAAAATGAAATGTTTTAATTCAATAATAAAATTCACAACAAGCAAATGTAGAAGGATCAAGGATAGTTTTGAGGCTAGTCAATTAATATATAAAAGTATTGATTGGGAACGTGTACACCTTCAAAAATGCATATAGTATTAATTGTTCATAAAAGGGAAATGAGCACAGTTAAGCACACTAATCAGCACCGAATCTAATCATGACCCGTCGATAATCGGTGTCGAATAGGCAACT

GGTAGGTCTGAACAGGTTGAACTAATCAAAGGGCACCTTGATCTTCTACAGCCGGCAAGTACTCACGGTTTCTGCTCCGACTCAAGCCGTTCCCTGGCCAGGGCGGCAGCCGCCTGGATAGAGGCGGGCACGTCCACATCGACGCCCTCCTTTATGTGCGACTGCAAGTGGCGCTTAAAGTTTGACAGCTGGGTGAAGCCCTTCTCGCAGTAATTACACTGGTAGGGCTTGGCGTTGAGGTGGCCAAGCTTGTGTTTCTTGAGGTTAACCAGCTGGGAGAAGTACCTGCCGCATCGCTGGCAGCGGAACGGCTTCTCGCCAGTGTGTGTCATCTTGTGGCGCTTCATGTTGCTCTGCTGGGTGAAGGACTTGTTGCAGAAGTTACAGGTGAATGGCCGCTCCCCGCTGTGCACCGCATAGTGGCGCTGCAGGTTCACAGCCTGGGCGAAGGCCTTCGAGCAAATGTTACACACGAAAGGCCGCACATCGTTGTGCACCTTCATGTGACGATCCAGGTTCCCCGAATTGCAGAAGCTCTTCGCGCACAGCTTGCACTCGTAGGTGGTCTTGTTGTGGTTGTGCACCTCTTGTCGATGCAACGTCTGATCCCGCTTGCTTCTGAAGGTCTGCATCAATGGATAGTGGTTAGATTGAGGTAATAGGAGACATTTCCCACACACTTACTCGGTCGCAGCGCTTGCAGCTGAACTGATTGGCCACCGTCTCGTCGAATTCTACCTCCATGCCCGCCATTTCGCCGTTGGCTAGCACATCGACTCTATCCGATTGGGAGTTCTCAAGAAGTGGACATCGAAAAACGGCAGGTCAGGCGACGATAAACTCACTATAATTAACTTATTCTGTAACTCGGCAATTTTTCGCGTCCAGAAAAAAGTCATATGGTGGCCTAGAGTGACCGTTTGCGTTGAATGAAAAAAGACCAGATTTAACGAGTACCGCTACAACTAGACTTGCATGTAAATTCAAAATATTACTTTGATAATAAGTACTTGGTATAACCTTTATCTTAATTGCATAATTTAAGTATATTCATGTAGTAGGTATTCCTCTTATGTTCTGTTGGGAACGGTGACTTGT

 

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