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CRIMIC CR01445

Gene CG42524

CG42524

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:15617821..15617846	52B2	FBti0210379	86393

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TGAGATAGGCATTGAAAGAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGGGTGAGGAATTCCAGGG	GCAAGCGGATGATCCCAAAC	CCGGCTTTGGTTGTGCATTT	TTGCGAAACGGCAAACATGT

 

Amplicon 5'

Amplicon 3'

GTGGGTGAGGAATTCCAGGGAGGATGGTCAACTGCCGGACCCGGTTTAATCAAATGTATTGAAAATGTTTCGATTCCGGCAAAGTTTTTCGAGTCACATGAAAGAGCCCAGGGACATCAAATGATTTTTGATCCTCGAATGGGCGAAGGATACAAAGCGAAGAAAAGAACAGGAGTAGGATGGTGTTAAGAGAATAAAGTTGGGCTAGGAAATTATAAAATACATTGTTAAGGAATTGTAACTTTGAGCTATGTATGTAAATCCGACTTAATCTCAGCTGCCAAAGTCTTGTATGCAGATGGTCAGTTTCCGGTGGTGCTATGATAACATTACGCATCCGCCGCATTGTCACTGCCACGAAAAGGAAATTTCCTCTCATTAAAAGCCAAATATGTGCTATCACAGCGACCCAAAGCAATAGGAGCCAAAAGCTCAGCAAAAAAAAAAAATAAAACAAATAAGAAATAAGAAATCAGCATACGGCGCACCTACAGTCGACTCCAGAGGACAGAAATATGTACATACATAACGATGAGCCTCACACGTTTGTTTATAAGGCCTAACAACAGTGAGCAGCGGTATATGGAATATGCACATACAAAGCATCTTCGACGACATCCCCCCCGCCCAAAATGGATCCCCAACTCCGCCCCACCAACGAAACGATACTTGTTCAATAACCGATAACATTTCGCTTTTGCTGACTTTGACTTTGCATCGTCTCGTTTTGGAATTTGTTGTGCTGTTTTATATTCGGTTCGTAGTTTTGCTGATACCGAAGCTTTAAACGCAAAAAATGCAAATTTTATTTCGCTATTCCGATGGGGAGGGTTTGGGATCATCCGCTTGC

CCGGCTTTGGTTGTGCATTTGGAATTGTTTTGTTTTTTTATTCGGCTTGTGTACTCATTTCTTTCTATTTGTTTTAAAACGCCGCTTACCCCGATTTATTTATTGTGCCATTCATGTTTCTCTATCTTTATGTGTGTTTGTGTCGCCCCTTTGTCTCGCCCCCCCCTCTGCAGTTGTGTTTTGTGTAATGGCTGCGATATTTATGGCCAGCAAGTCCTTCGTCCTGCCGACTCGTTTTGGACTCCTAGAACCAGCAACTGCTGCAATTTATGACCATGGCGAGGATTCGGCATCGGCATTGGCATCGGAATCGAATTCGCATTTGGATTTCGTATCCCTGGGCTAATAAAATATTGAAATATATAACAATTTGCATGATATTCGGTTCAAGTTCTCGGGGAGAGTTGAATAAATATGCCATTCCGCACCGATCCGGGGCAATTGTTTTCATGCTGCACTCTATATATTTCGCAGCCTTTGGAGTGCAATTTGTGTCGGTAGGAGGCTTTGATTGCAATTTCAGTTTGTAGCCCGAAAAGCCCTAATTGACATCGGAGGCCGGGGAGCTCAAAGTGAGGTCCTGGGGAATTGCGAATCCATTACTAGTCCGAGCACAGCTCTGGCTCGAGCTCTCTTGTCTTGTCAAGTTTATGGCTTGGCCAAGCGGTCAGCATCTCAATTGGCCGAGAGATGCTCTTTCCAATTCCCGAATTCCCATACCGATAGTGAGCATTTGCCAAGCCAAAGATGATGCCGGGCCCATAAACAAAGAGTTTGAAATGTTTAGCACGAATAAACGGCGGCTATTTTCATTCGCATTACGTCAACAAACGCAGATGTTCCGATTTTAGCGGCCGGCACATACTATACATATGTATGTTGGTGGAGATAGATCCACATGTTTGCCGTTTCGCAA

 

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