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CRIMIC CR01446

Gene ana

CG8084

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9071618..9071638	45A1	FBti0210380	86394

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	TGTCTGCCCCAAGCGAATGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTTCCAACCAACTGACCACA	GCTTGGGGCAGACATCTCTG	TTTCCCCCTGACTCCAAAGG	TCGGAAGAGTGGCAGACAAC

 

Amplicon 5'

Amplicon 3'

ACTTCCAACCAACTGACCACACAATATCTAAGTAGATCAGAGGGGCGGGGGGTCGCCAGCCCAGATCCATCAATGCTATCTGTTGTATCCATCAGATACTGTAGTGCAACAGTCGCGACGCCGCTGCACAATGCGGACACACTGACACAGGCACACTGCAACACACAACTGCAGAATGCATCGAATTCCGTCGGCGACCTCTGTTTCTTTCTATGGCTGTCTGGCTGATGTCTGGGAGCAGTCTGGGCCATGTCTAGCTATCCGGGCTCCGAATGCGACTCCGTCCGCGAATGCGAATGCGACTGCGGCTCCGTCTGTGTCTGTGTCTCCGGCTGCGGCTATCCGGTAATGAACTTTTTGTAGGTCGTTGCTATGCAAGTCGGGGACTCGTGGGGGCCAACAGCTGATATCTAAATTGTTTCATCTCGTTTGATTGCTTTGGCAGAGTTCCGTACCCGTACAGACGTACAGACGGCGAAAAAAAAAAGAAAAGAGGCAAAAAACCTATGACGTATGAGTAATGTCATCGGGACAGCAGACATGTCTGCGCCCCCAGCAACTGGCGCAATGGTGCAATGGTGCCTTGTTGCAGGGCGAACGGCAGTAGGCGTGTGCATTTCGACTGGCTGCAGTTTCGCCGTTGGTTGCATGATAATGAAGTGCAGCCGGCGAGACACTGTCTAGCCGTTGTTGCTACTGTTGCTGCAGCAGTAGCAATTGCTACTGGTGTTGCTGCCACTGTTGCTGCTGCTGGGGCCTGAGGCAACGGAGTGCAAAACCGCAGACGCAGACACTGAGGCGATGCATTCAGAGATGTCTGCCCCAAGC

TTTCCCCCTGACTCCAAAGGCAACTACCCCCCAAAATGGCACCCTGCCTCTGCGCATGCGTGACTCACGCATGAACTCAAATAGTGTCTAAGCGGACAGATTGTCACGTTAGCATCGACAGTACACTGGGAGAAAAGTGGCAGTCGCTGTTGGCTTCATTAAGCAGTAAGACTCTAAGCCAACCTCCTATGATGGGTCGGTCCATTGAGTAATGTGTCTCAAATGCATCTTCAGCTTGAACAATAACTATACACTGATGAATGTAATATTTTTATAAATTTAATAGTAACTAAAGTGCTACCCCCTGTACCCCCACATTTATTGATTTACTCCTTTAGGTCTGTTCCAAAATTATCCATATTGCAAAGACCCCTGCTGTTCGACATGAATCAGTTTCAGATTGACCCCGATTCCGTGTATTTTTTTTCGGTGCAGCATCTAGGCCGGCAGACAGACAGACAGGGACAGGGACAGGGACAGGGACAGGGAGACAGGGAGGCAGGCAGACATAGAGGCAGCGAGCAAACAACGAGCGGACGGAAAGACCGCCTGTCTGACGGACCGACAATCTGTCTAGACGGTGGCCAGACAAACGGCACGACTCAACGAGTTTTCAAGTTCATGCCACACAGCCAACTCCAGACGACTCCGCATGCTGGAATTCCTTGGCCAGCCCAGCTAGCCCGCTAGCCCGCTAGCCCGCTAGCCCGCTAGCCCTCTAGTCCCCTAGCATGCTAGCCAGCTAGCCAGACGGATTCGGTGCGGTTTCGGTTCGATCGATGCGATCACAGCGTAAACATAAACATAAACAAGAACGGATAATCAGTCAGGGGCTTGGTTCACTTTCGGAACGGACCAAACGGCCGAAATGCCAAGAGCCCGAAAACTGGGTTTCATAATTATTTTTTGGCTAGACAAACTGCGCCGCGGGCCAGACCAAAAGCCAAAACAATTAATCAGTTTTTTTCTTCACTTTTTGATCTCTTTGTGCATTTCGTGTGTGGGAAAAGGAACAAGACGAAGCGACAGGCGCGTCTCAATTGCCGATCCAGACGCATACAATTGTCCAATTTCCAGGCGAGTGAGTGCGGCCAGATTAGATCATAGCTAACTAACTATTGTTGTCTGCCACTCTTCCGA

 

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