CR01448

CRIMIC CR01448

Gene Keap1

CG3962

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:17075429..17075446	89E11	FBti0210381	86395

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GATAGAATAAACAACCAGAAAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTATTGTCATGTGGCTGCG	TCGACATAGTTTAGCAAACCCT	AAAGGCTTTGTCAAATTA	ACAATCGACACTGTAAAATG

Amplicon 5'	Amplicon 3'
CGTATTGTCATGTGGCTGCGCTCGTGAGTTTTCGCAGGCGACCTTCGATGTGATGGTGAAAAACTTTCGCTGCTGTGCTAAATAAACATTCTTTTTGTGCTTTTTTTAATTTATGCCGCATATCATTAGCATTTCTCTCATCATCTGTGAAAGACGCTGGTGGAGTTCTTCGAAATCTTTCCTGCATTCTGGACCAAGGACAGTTTGGATTGACTAGCTCCTCGGATCTAATGGCGGGAATTTGAAAACTATGGTGTTCTGCAGGAATATATGGTTTTATATCCATTTCCGAGTCACAATTGTTGCAGCCGCTCATTCCGTATGAAGTATGAAACTGCATATTGGACGCCGTCCCAGGATAATGTGGACTCCCACTGCCGTACGTGTTTTGTGGTGTATGAGCATTCCTTTCCCCGTTGTTACTTCCATCCGGGTGTGGGGCGTCACTATCTTCGTAGTCCATAAACGTGGTGGAACACGCCGGCTGATAAATAACCGCCGACGCATGACCCGATCTTCCCGATTTTAGTGGTGTTCCTGTTGTCCACGTATTTGTTCGTGGATCGTAGACCTCGACTATGGACAGAAAGTTGTTGCCATCGAATCCACCAATGGCGTACAGTTTTTCGTCCAGCGGAGTCAGCGAGAGAGCACTGCGTGCGATTTGAATTGGCGCAACCATGTCCCAGGTATCGTTTTCGGTATCATAACGCTCCACGGTGGCTAGTTGACGGGTGCCATCGAAGCCACCAACAACGTAGATGTACTGGTTAATTGCAGCAACACCTGATAGCAATTAAATCGGAACTTAGTTGGAAATTAGATAACAGGATGCGCCAGTCATTCTTACCCGCTCCACTGCGACCCGTTTGTAGTGGTGGCAAAAAACTCCACTCATTGTTCTCCGGATGGTAGCACTCCACGCTGGCCAAGCGCTCGTTTCCATCGAAACCTCCGATGGCATAAAGCAGACGGTTGACCACCACAACACCCACTCCCAAGCGCTTGGCATGCATCGGCTGTACAAGAGTCCAGCGATCCAAATCGGGGTCATAGCTGCAAGAGATTTAAATAAATAGGGTTTGCTAAACTATGTCGA	AAAGGCTTTGTCAAATTAATTAAACTATTATCTATTGAAATGATGAACACTACTTACTATTCCACCGTGTTGTGGTACTCCATGCCGGCGGACCCGCCTACGGCGTACATCAGCTCGTCCATCACGGCCACGCCCACTCGATGGCGTGGCACGGACATGGGAGCGCACGGACGCCATGTTTCGGTGACTGCACTATAGCGATCCACCCAATCCGAGTCGTAGGAAGATCCAATGTTGTTATTTCTGCCTCCAACCGCATAGAACTTGCCCTTGAGAAAGGCGGCACCCAAACCAGATCTTGGAATGCGTAGATTTGCCAGTGTCGTCCACGTCATGTCGTCTACATTGTAGGCCTCCAGAATGTCCAGTGAGTGACGAAAAAAGCCACCGGCGACAAATATCATCCTTGTGGTGTTGGGGGTTCGTTCCTTGACTCCTGGACACTTGTGCAGCGTTAAGTCCTTAAAGATCTTGGCCAAATACTCCCGACAGGCGGGCACCTTTCGCAGAACATCGCAGTTTTTCATCTGCTCCTTGAGGAAGTTGGGCGTAAGGAACTGACAACGGACAGCGCCCAAAATGTGCTCCATTTTACAGTGTCGATTGT

Amplicon 5'

Amplicon 3'

CGTATTGTCATGTGGCTGCGCTCGTGAGTTTTCGCAGGCGACCTTCGATGTGATGGTGAAAAACTTTCGCTGCTGTGCTAAATAAACATTCTTTTTGTGCTTTTTTTAATTTATGCCGCATATCATTAGCATTTCTCTCATCATCTGTGAAAGACGCTGGTGGAGTTCTTCGAAATCTTTCCTGCATTCTGGACCAAGGACAGTTTGGATTGACTAGCTCCTCGGATCTAATGGCGGGAATTTGAAAACTATGGTGTTCTGCAGGAATATATGGTTTTATATCCATTTCCGAGTCACAATTGTTGCAGCCGCTCATTCCGTATGAAGTATGAAACTGCATATTGGACGCCGTCCCAGGATAATGTGGACTCCCACTGCCGTACGTGTTTTGTGGTGTATGAGCATTCCTTTCCCCGTTGTTACTTCCATCCGGGTGTGGGGCGTCACTATCTTCGTAGTCCATAAACGTGGTGGAACACGCCGGCTGATAAATAACCGCCGACGCATGACCCGATCTTCCCGATTTTAGTGGTGTTCCTGTTGTCCACGTATTTGTTCGTGGATCGTAGACCTCGACTATGGACAGAAAGTTGTTGCCATCGAATCCACCAATGGCGTACAGTTTTTCGTCCAGCGGAGTCAGCGAGAGAGCACTGCGTGCGATTTGAATTGGCGCAACCATGTCCCAGGTATCGTTTTCGGTATCATAACGCTCCACGGTGGCTAGTTGACGGGTGCCATCGAAGCCACCAACAACGTAGATGTACTGGTTAATTGCAGCAACACCTGATAGCAATTAAATCGGAACTTAGTTGGAAATTAGATAACAGGATGCGCCAGTCATTCTTACCCGCTCCACTGCGACCCGTTTGTAGTGGTGGCAAAAAACTCCACTCATTGTTCTCCGGATGGTAGCACTCCACGCTGGCCAAGCGCTCGTTTCCATCGAAACCTCCGATGGCATAAAGCAGACGGTTGACCACCACAACACCCACTCCCAAGCGCTTGGCATGCATCGGCTGTACAAGAGTCCAGCGATCCAAATCGGGGTCATAGCTGCAAGAGATTTAAATAAATAGGGTTTGCTAAACTATGTCGA

AAAGGCTTTGTCAAATTAATTAAACTATTATCTATTGAAATGATGAACACTACTTACTATTCCACCGTGTTGTGGTACTCCATGCCGGCGGACCCGCCTACGGCGTACATCAGCTCGTCCATCACGGCCACGCCCACTCGATGGCGTGGCACGGACATGGGAGCGCACGGACGCCATGTTTCGGTGACTGCACTATAGCGATCCACCCAATCCGAGTCGTAGGAAGATCCAATGTTGTTATTTCTGCCTCCAACCGCATAGAACTTGCCCTTGAGAAAGGCGGCACCCAAACCAGATCTTGGAATGCGTAGATTTGCCAGTGTCGTCCACGTCATGTCGTCTACATTGTAGGCCTCCAGAATGTCCAGTGAGTGACGAAAAAAGCCACCGGCGACAAATATCATCCTTGTGGTGTTGGGGGTTCGTTCCTTGACTCCTGGACACTTGTGCAGCGTTAAGTCCTTAAAGATCTTGGCCAAATACTCCCGACAGGCGGGCACCTTTCGCAGAACATCGCAGTTTTTCATCTGCTCCTTGAGGAAGTTGGGCGTAAGGAACTGACAACGGACAGCGCCCAAAATGTGCTCCATTTTACAGTGTCGATTGT

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