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CRIMIC CR01450

Gene CG43066

CG43066

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:18519783..18519807	55D1	FBti0210382	86396

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTACACAGAGCAACAAGGTTCAGGA	TGGCGTATACATGTGCATCAGTCCAG

Images

Construct Design Sequences

sgRNA sequence	CCAATTTAGCGTTATCACTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGGAGAATCCGCCTAATGG	GACCCGTGCAGTCAAACAGA	GATAGACCCCTGCCACGC	CCAGAAGTACGTCGTCTCCG

 

Amplicon 5'

Amplicon 3'

GGGGAGAATCCGCCTAATGGCAAACAATTAGGACACAAATTGAGTTAGCAGCCACTAGAAGCGGTCTCAAGGACGAACCGCTACACAAATGGGCAAACAAGTCCACACACACAAAATAGCACACACTCTGTTACTCGGGGAGTATCGATCAAACAGTGGGTCTGGGTCCAGTCCTGGCACTTCTCATAATCGGCTGCTCCACTTTTCCGCAGACGGTAACCACGGACACTTCGCCAGGAATATCCGCACGTTGATGGCTGCCACCAAAATCATAGATGCGCCGCGCAACGGCCACGAGATGGCGCCGCTGAACACACGGGCACGGGGCGATGGAACCCACGGGGTGACCATCGTCCTTACCGCCCCCCAGCGCAACTCCGTGCAATCGGTGGACATTCCGGGCAATGAGCCGGAACGCGCCGCCTGGAGCGGCAAGATGCAGTTCTTCCTCAGCATCATCGGATACTCGGTGGGCCTCGGCAATATCTGGCGGTTTCCCTACCTTTGCCAACAGAATGGAGGCGGTAAGTATTTAAAACTATTTAAAAAGGAAAGATGTTGGTTTTAGTTCGATCAATAAGAATGTAATGAATGAATTGAATGTGTATAAATATTTCAAACAGCATTTCTTGTGTTTAAAGATATAAATAATAGATATAAGATATAACTACAGTTCTATGTAACTTATTACTTTATATGTTTGGTATGCAAAAAATACCTCATCGATTACGAGTTATGATTGTTTAGGTCTATGCACTTTTGCCTGGCATTCAACGCTAAGCCTCGGATTACTGCGGCAAAAAGCGCAAACTGGAGCAGTATTTTCAAATCAATGTCTGTTTGACTGCACGGGTC

GATAGACCCCTGCCACGCCCCTCCACGCCCCCATCGTCCGGCGACATGTGTCATATCCAATATCAGTTGGCCAACGAACTGCAGTTAATCAGGTGCACAGCTTTGACAATAACATTATGGGATTTAATGTGCACTAATTGCCTGGCATTCGACGGCGGAAGGGCCCAATTGATGGCCTTGGGTGGGCCAGTGGGCGTTGAGTGGGCGGGGTGACTTGTGTGGGTGGAAAGGGGACGTTATAGCAAGTGTACGCACATTTCTAATGGCGATTTAACCCCACCCGGCTTTTAATTCAATTACAATTGACAGCTTGACCAGGCATCGAAGTTGGCATACTCTTTTCAGCTAGTTGTCCAACCAGATATTTCCCGACCTGGCCAGAAATGCAAATTTCCGGCTGGCTTTGAATTGATTTTGTGTCACATCCCCAGTAATTTAACCATGCACTGCGAAAAAATTACACTATGAAGTGCAAATATGTTTATTCGATTTAAGGCATTTCATTAAGATTTTATATAAAGAAAGTTCATGAGCTAATTTTACAACCTTATATAAAATGAATTTATAGATATTCTTAGTATTTCTTTAAGTGAATCTAAGCCTTCAATTTTTCCGTTGCAGGTGCATTCCTGATACCCTTTATGGTTATGCTGATACTGGAGGGCATACCGCTGTTCCTAATCGAGTTGGGAATCGGGCAGAGAATGCGATTGGGTGCCTTGGGTGTGTGGAACACCATCCATCCCTGGCTGGGTGGCATCGGAATCTCGTCGTGCATAGTGACGCTATTTGTGGCTCTCTATTACAACGTGATCATCACGTGGGTTTTCTTCTACCTTTTCAACAGTTTTCGGGTAAGTGATTTTTATTGAAAGCAATCAGGATCTATAATTAATTGTTGACTTCTTTAGTATCCGCTGCCCTGGTCATCTTGTCCATTGAACGGCACAGGATTTGAGTTGGAGGAGTGCGCCAAATCCTCGGAGACGACGTACTTCTGG

 

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