• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01451

Gene CG6362

CG6362

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:17772311..17772319	54E8	FBti0210383	86397

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CTGGCAAAGACAAGACGCGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCTCGGAAGCTCTCGTGG	CGGATGTTCCATAGCAGATACT	TTGTCTTTGCCAGCCAACTG	ATTTGGCTGGCGCACATTTT

 

Amplicon 5'

Amplicon 3'

GATCTCGGAAGCTCTCGTGGTATTCGGGAATCTCTTTGGTAAACTCCCCGCGCGGACGCAGGTGGTCGTCCGTCTTAAGGGACTTCTCTTTGGGCACGTTCTTGGGTGGGTCGATGAATTTGTCGCGGTACTCCGGCACCTCCGAGCTGGGGGCGCAGGCCGCCTCGGAACCGGATACGCGCAGGTTGTCGTTCTTCTTGATGGGCGCACTCTTCGAGTAGTTGGCATACATACGGAAGCTGTCCCTGTATTCCGGCACCGCCACGAACTCACCCTGAATCTTGATGCTGCTCATCTGGGGAATGGAGTGCGCCTTCTCGGGGAACTGCTGCACGAACTGGCGACGGTACTCGGAGGGCTCTGGCACCTGGCCCTGCTGTTCGTGGTCCACAGCCTGGGCACGCATCTGCAGATGGCATCGCTGCTGCTCCTGGGCAGCCACATCGCGGCGAAACTTGTCCGCCGCCAGCTTACTAGTGGGTTGGTTCCGGGCCGCCAGCTCCGACTCGTCGAGGAACACCTCGCGTGGCAGTATCTTGGTGCTGGGATCCAGCATAAGGTACGAGGAGTTCTTCATGCCATCGGTGGGACCGCTGCCCGCGCCCAACTGCAGGTTCTCCGGATTGCGGACGGGAAAGGTGCGGGAGCTGTGGGCAGAAATCAGTTTTCTATTGATTAACTATTGATTTCTTTGAAAGCCAGTTCCCTGCCAGTTGAACTACTCTAGACTATTACTAAGAATTGGCTACTCATCTACTCACAATCGCAGGTCCTCGGGCGTGTACCAGTGGTATTTCTCCTGGTGCTCCGACAGCAGCTGCATGAATCCCTCCAATCGCAGCGAAGTAGCGCGCCGCAGCAAGGATCTGGGTCGGGACTGGGTGCCACAGAAGTGCTTGCGATACTCGGACTTCAGGGAGCCGAAACTGATCGCTGGCTCATCGCCGCTGTGGAGGCATACGTTGCTCCGGGTGTGCAGATGGACGGGCTGGCGACGGCGAAAGTACTTGAGGTCATCGACGGCTATGTACTCGTGCGGATGCTTGATGGCGAACCGGCTGAGATCGCCGGACTTCTGATTGCCTGTGAGATCCGGCTCGGAGAAGGAGCGATTCCAGCCAAAGTCGGCATCAAAGCTGTGGTATGTGGGGGATAATGTGAATCCGGTTAGTATCTGCTATGGAACATCCG

TTGTCTTTGCCAGCCAACTGAGTGGAAAAGTTATGGGTATCGCTGGTGGTCGCCACCACAACCAAATAACAGGTAGGTCCCTTAATAACGGGGCCACCAATTCCCCCATCTGCCTTCGACCTTTTGCAATTGCAATGGAGGAGTTTTGGGAAGGAGGTGGGCCTCCATAAGACCACGACCGGTTGCAAAAGGCAGCCAGCGAAGGCATTCCTCCACATCCACCTCCATTTCCATAGAGCAAGACCCGATGGCGTGCTATGACCATGTTGCTAGGCACACTCGCCTCACAAACCTCTGTAAACAAACTAATGAATGAGGGAAAAGGGTAAAATTGCTGCAAATTAGAAAATTGTCTATTGGATTCGATGGAAGCAGCAGGTCGAGCAAGCAGAGTCGATACGATGGTATCCATTGCCAAATCCCTTATTAAATCACTTCCAGGAAAATTGAAAATAATCATAAAACATAATAATAATAATACTTTTGTTATCAGAAAGACCGATCCCAACTCAAAATATTTAACACATGCATTTCAAAACTAGTTTCTGAGCTGGCTTAAAAATGATACCCAAATTCTGTACAGAATGTTTCAAAAATCATTACATCCTTTTTTTTTTAAACACTGTTCCTGGGCATATATGAATAGCTTGCCCACTCCACAAAATGCCAATCAATAGCTCACGAAATTCCCGACTTTTGTTAGTCCTGACAACGCCCACGACACTGCGACTACAAGGACCTTGGTAATCCATATACGGAAATGGTTTTTTTTTTGTTAAGGCATTGCCAAGGACTCGTCGTCTCCCAGCAGGCAGGCCATTTAAAATGCACAAGGATTGCCAGTCGGAGGCGAACCGGCGCGCATGCAAATTGCACTTTTTATGACTCCCGAGACCGGACTAAATGTGACCACAGCCACCTGTTGCGCAGTTCGGAATTAAAACAGTTTTAACGACGCCTAAAAGTTCTCACGTGTGGAAAAATGTGCGCCAGCCAAAT

 

---

Search Again