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CRIMIC CR01454

Gene NimC2

CG18146

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:13981587..13981607	34E5	FBti0210385	86399

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGATTTCAGAAGTCCCTTTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATTGTGGAGCCTCGGGAAA	TCGTCTGTTTAAGTCGTGGGA	TTACGGTACTTCATCCCCATTT	TAGCCGGGAACTTTGCTTGT

 

Amplicon 5'

Amplicon 3'

GATTGTGGAGCCTCGGGAAAATGCCTTTTGCCCAATGTGTGTTTGTGTGGAAAGGGTTATGTAAGTCGTAAGGATCACGGTCACTGTGAGCCGGAGTGCAGTGAGTCCTGCGTGAACGGAAAATGTGTGGCACCGGATGAGTGTGAATGCCTTGCTGGCCATCGTTTCGTGAATGGCTCTCAAACGGCATGTGAGCCAATTTGTGTGGAGGACTGTGCCAATGGTCGTTGTCTGGAGACGGGAAAATGCCTGTGCAACAATGGCTATCAGCGGGATGAAAAGCTTAAGAAATGTGTGCCCATCTGCCAGGATGCCTGCTATCATGGCGACTGTGTGGCGCCCAACGAGTGTCGCTGTCATCCCGGTCATGAGCAGCGCTTGGGAGTGCCGTGGATTTGCGATCCGATCTGCTCGAGCGGCTGCGCCAATGGCTATTGCCAGGGTGCCGAGGTGTGCGCCTGCAAGATGGGCTATGCCCACAAGGATAACACCCTTGCCTCAGGCTGTGAGCCTGTGTGCAATCCACCCTGCACGAATGGCACTTGCATCTCACCCGGCCACTGCGCCTGCTCCGAAGGTCACGTATTTGCCGAGGGATCGCGTCACGAGTGTGTTCCCAGCTGCCGTTCCGGTTGCGAGAATGGATATTGTAGTTCTCCTGGCCGCTGTGAGTGCCACGAGGGATTCGAGAAGACATCGCCGCACCGCTGTTCACCCACATGCCGACCGGGCTGTGGCCAGAACTCCCGCTGCGCCGCTCCAGACACCTGTGCCTGCGATGTCGGCTACGTCTTTGTCAACGGCAGCACCACCGAATGCGAGCCCTTCTGTCCCAGGAACTGCCGGAATGGAATCTGCAGTAGTCCAGGTGTCTGCACATGCTTGGAAGGTTTTCAGGTGGGTAATAAAACTATATTCCTAGTTTATAATCCCACGACTTAAACAGACGA

TTACGGTACTTCATCCCCATTTCTTAATGAAATCTTGATATATTAATTAAGTGTGTATTTTTTATTAACAGGCCCTTCTCTCGTTCTACTGCATACCAGTTTGCTCGAAAACCTGCATTCATGGCAGTTGTGTGGCGCCCAACGAGTGTCGCTGCTTCACCGGCTACCGACCAAATCCCAGTCTGGGAGCGAATGTCTGCGAGCCCATCTGCAGCCAGGGATGTGTTCATGGATTTTGCATCGCCCCAGAGATCTGTCAATGTGATCTGGGCTTTATCAAACGATGGGCCACTGGGACCTGTGAGCCGCACTGCCCACAGAAGTGCGTGAACAGCCATTGCTTGGGATCGGGAGTGTGTCGCTGCTACGAGGGATACAAGCTGAGGCCGGGATCAACGTCCATCTGTGATCCGGAGTGCCAGCCAGGATGCAGGAATGGAACGTGCGTAGAGCCCAACAGCTGTGCCTGCTTCGCTGGCTACGAAGACACCAAGGTGCCGTACGAGTGTGTACCCTCCTGCCGTCCGAGATGCGAGAATGGGCGATGCTCCTCTCCGGGACACTGTGAATGCGATCCCGGCCATGTGGTTACCAACTCCAGTGAACCGAACTCATGTCGTCCCCAGTGCCAGGAGCAGTGCATTAATGCCGAGTGCGTTGCGCCGGAGAAGTGCGCCTGCCTACCTCGTTACCGATTTTTGCCCGACAGCAGCACCGAATGCGAACCGATTTGCTCGAAAGGATGCCTTTCTGGGCAGGAATGCGTGGCACCAGACACCTGTGCGGGCTTTGAATCCCTAATAAGTCCGACCACTGGCAAACACCTGGCATTCCATTGGTCCATGTTTGTCCTGGCAATTTTACTCTGCCTGGCTCTGGTAATGATTCCCCTGTTGGTGCTCCGGGAAATGCAGCGACGTCGTCGTAATGGCGACAAGCAAAGTTCCCGGCTA

 

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