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CRIMIC CR01461

Gene CG17544

CG17544

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:19393440..19393447	37D4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	GCCTGTGTTCACCAGGTACATGGAAT	TCCTGGTTCGGTGAACACACTTTCGT

Images

Construct Design Sequences

sgRNA sequence	AAGCTGTCACGTAAGACTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGGTGGTCGGATCATAGGT	TTACGTGACAGCTTATCAAATCG	TGGAAGATATTCCAGCACGTTCT	TGCCTTATGGTCTCGCGATC

 

Amplicon 5'

Amplicon 3'

TGGGTGGTCGGATCATAGGTGGCCGTTGTCCGGATGCTCTTCGTGTTGCTGCCATGCGATAGCTCCGTGATGGCCAGGCAGGTGATAACCTCCCGATTCCAGGCTGCCTCAATATATTTCTGGTGCTTCTCGGTTCCCATGGCACGAATGGCATTGTTAAACAGGCCCACGCCTAGGGCTATCTTCACCGACAAACTGGGCGAGTAGCAGGCGAGCGCCTCGTTGATGTACATCAGATACTTTGTTTTGGCCGAGAAGCTCAGGCTCTCGATCTCCTTGGGCACCAGGTCCAAGTGCTTCATGCGATTCACCTGCATGGCGCACAGGCGCTTCTGCTCGTCCATGGGCAGTGTGCGGGATGGGTGGGCAAAAAGGGGGTCATTCTCCAGGCGACGCCACACTTTGTACTACGAAAAAGGGGACATATTAAATAGGAATTCTTTTAAGAATTTAATAACAGGAGTAAGTGTGGTAACATTCTTAGATTCAGTTATATATTTGTTATACATATGTACGCAAAATTACTGAAGGTAAGGTAATATATCAAGTATCCTAATTATTCAACAAACTTCATAACATAACTCACATGCATAACTAATTATGCTATTTGAAATTCATTATGTATAAAAATTATTTTGAAAACTATAATCCAAAATAAAAGTTTGTTTTATAACGGAAAATAAAAGTGAACATTTTATTTAGCGTGTTTTTAACGCTTTATAAACTATAATAAACTAAGATTTTAATGGAAGTCGCAAATCGTACGTAACAACTACTTATTGGATAATGCCATCAATTTACTTTCGAGACTCTAAAGACATTAGCCATAATTCAGCGATTTGATAAGCTGTCACGTAA

TGGAAGATATTCCAGCACGTTCTCTTTCTAAGAGATAGCCATCCACTTACCTTAATTCGCAATCCCTGCTCCTTCTCGAATATCAGTCGCAATCGCTTCCAGTCGAAGTTGGCCCTCTTCCTGTAAACGGCCAATGGTCCACCGCTGGGAAGATCGGGAATAAAGCTTGTGTCCTCGCTGCCCATTGTGTGATTTTGTTTGATTTGATTTCTTGAAGAAGAAAGGAAATGGCAATGTTATCGGATCAGTGGAGCTGCTTATAACCAAGAGGCTATAGAACGACTGCGATCTTAGCCAAACGCTTAAATGGCCCAAACCAACCAGTTGTCTAGTCAGTCAACAAGTGGGGCACTCGAACTGTTTTATCTACAAGCGTGCAAACGACTGAAGCCGTAGAAAGGAAAACCCTCAAGAGGTGCTGGTTTTGAAGCCATTGTTGGCCAGCAAAACAGTTTAGCATTCACTGTTCTCAACTAACTATCATCTTATGCACAAAGAGAAGTTTCAAACCAATGGGAATAGGCTTAGTAAAGTTCTGCGCCCAAAATGAGAACAGAATATAACAGAATAATTGGTTTTAAAACTTTATGTTCTATTGAATTTTGTTCTAAATATATGGATACATTAATTGCTAAGCTACATTAGTTATTAGCAGCAATTCTTACAAAAGATTACTTAGTCGTCATAAGTAAACTATTAAATATACTTCAAAGCATAACTGCCACAAAACTAAATTGAAAATTTATACATTTAATGCATCACTTTTAACCTGATCCAAGGTCATGTCCTTGCAGTGGCAAAATGAGGTGGTGCGGATTACGCGCCAAGTGAAGCGCAATCTACCGCACCGAAATTTCCGAGAGATTAACTTTGATCGCGAGACCATAAGGCA

 

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