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CRIMIC CR01462

Gene CG31638

CG31638

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6493096..6493114	26D9	FBti0210388	86402

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GATATACACGCGTTGATCCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATCCATGCTGCAATTGCGC	CGAGCCATCACCGGACTG	GTATATCGTGCGTGCCCTCA	GTGGATACTGTCAGGCCGAG

 

Amplicon 5'

Amplicon 3'

TATCCATGCTGCAATTGCGCCTCGACGAGGCTCAGAAAACTCTTCAAGCGGAAAGGGAGTGAGTAATCTCTATTCCAATAATAATACTTTAATTATTTCTATTATTTTCGCAGCGAAAAGCTGGAGCTGCACAAGAGCATTGAGAAACTGACGCTGGAGATCCAGGACGTGCGCGGACGTCAAGAGGAGATGCGCTCGGCCAAGCAGGAGGCAGTGCGTGAACTATTAACTCTTCAAGAACAACATCGTGCTGAGATGCGTATCGTGAATAACTCGCTGCAGGAGGAGATTGCTGCCCGTGAGAATCTCGAGCGCCGGTAAGCAAATACGCATTCTGTACTTTGACAAGAGTGTAACTATCGATTCATTGCAGCCTCACGGAGCTTCGCACTGAACTGGAGCACCTTCAGGCGGAGAACGCCTCCGAATGGGGCAAGCGCGAGCGACTGGAGTCCGAGAAATTGGCCATGGAGCGTGACAACAAGAAACTGCGCGCAGAACTGCGAGATTATCAGGAACGTTCGGACCGTAAGTGCCGACCCATGCAAGCTAATGACGTGGAGCTGCGAGCATTGCAGCAGGAACTTTCGGAGCGCAACAAGGAGATCAGCGAGGTGAAGATGTCGCACGCCAAGCTAAAGAAGTTGCTGGCCGAAACAAACACAGAGCTGGGACACGCCGTGCGGCGAGCCGAGCAGTATGAGGCGGAGGTGAGTAAATCCACATCCAAAAATCTTATCAGATGATATATGGATCAGATTACGCGTATCAGCTTATCAGCCTAGGAAAACTTAAAGATGTAACAGCCTTCCTCGCCGAACCGAATTCGGTTTGATTAACATTTATTCACATTGACACTTCGACATAAAACAAATTGGTAAATGCAGTACTTTATGATTTTCGAAACAAGTACATTAGTTAGCAAATGCCGCCAGTCCGGTGATGGCTCG

GTATATCGTGCGTGCCCTCATAGGTGTTAACTGACTCCAGGTTGATTACATGGCGGATCACATGATATTCGTCGCTGATTCCGTTTGCACCCAGCATGTCCCTCATTTGGCGCGCTATATCAAGCGATTTGCCAGTATTGTTTCGCTTTAGAAGGGAAATCATGTCGGGCGTGTGCAGCTTCTGATCCTTGAGGCGACCCACATGCAGGCAAGCCTGCAGACCCAACGCAATCTCTGTGATGGCATCGGCCAGCTTCTTTTGGATCAACTGATTTGCAGCCAAAGGACGACCGAATTGTTTGCGATCCAGTGTATACTGGCGAGCAATCTCCACGCAGGTTTCAGCTGCTCCCAGGGCTCCCCACGCGATTCCATAGCGAGCGTTGTTCAGACAGCTGAAGGGTCCACTAAAGCCGGCCACATTGGGTAGCAACTGCTCCTCTGGCACACGGACCTCGTCCATCAGGATCATACCCGTGGGCGAAGCACGCAGAGAGAACTTGCCCTCGATCTTTGGCGTCTCCAAACCCTTGCCGGATATCTTGCGATCCACCAGGAAGCCGCGTACCTTGCCATCCTCGCATTTTGCCCATACGACTATGACGTCTGCGATGGGCGCACTAGTAATCCACGTCTTAGATCCGTTCAGGATGTATGTCTTGCTTTTGCTATCGTACTTGGCACGGGTCTCCATGCCAGCGGGGTCACTACCGTGGTTGGGTTCAGTTAGGCCAAAGGCACCTGAATAAATATGTAAAGAAATTGAGATTCGTTTAAAGAGAACACATATAAACTACGGGACTTTATATAATTCACTCAATATTTCATTAAAATATGATTTTATTCATTTGGCACTCACCAATCAGCTTGCCCTCCGCCATGCTGGGCAAATAGCGTTGCTTCTGCTCCTCGGATCCAAAGTCGTAGATGGCGCCCATGGCCAGCGAGCTCTGGACACTGACAGCAGACCGATAAGCGGAGTCCACGCGCTCCACCTCGCGGGTGAGCAAGCCGTAGGCAACGCTAGAGACTCCGGCGCAGCCGTATCCCTTAATGGTGCAGCCGAGGACACCCAGACTGCCGATCTCCTCCATAATTTTCTTGTCGAACGTCTCCAGGCGATTGGCCATCTTGACGCGCGGCTGCAGCTCGGCCTGACAGTATCCAC

 

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