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CRIMIC CR01463

Gene CG4409

CG4409

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16444186..16444211	53C4	FBti0210389	86403

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK

Images

Construct Design Sequences

sgRNA sequence	ATACGCGTAGCCGTAGGAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTCGGTCATGAATTCCCCC	ACAAAGCGTGTCCCCTGG	GCTACGCGTATGTTTGGGGA	CCCATCGATTTCCCACCCAA

 

Amplicon 5'

Amplicon 3'

CCTCGGTCATGAATTCCCCCAAACAAGTGAGTGTTTTTGTTGAACGTTACTGATTAAATTAAATAATCGCTCGGACAAAACGATTGTCAGAGTCTGGAATGGTCTCCAAAGTCTCCACAAAACTGCGGGCGAAAAAGTACCGTGAGAAGAGACTGTCAGAAGTTCAGAATCGGTACAGAAGCTCACATCGCCAGTATGAATCGCGGCCAGCTTTTAGTTTTGTGCATTTTTGCCACTGGAATTTCCCTTTCGATGGGTTTTCCCCAGGACGATCTAATTAAGCCGGGTAGTGACGAATCGGATGTGAGTGGCATTGGCTTCGTGACGCTCGACGGAACGGCCAGCGAAGAGAGTGACGAAAGCGACCAAGGCAACGCGTCCGATGATTTGATATTTTTGAGTCGCCAAAAGCCAAGTCCGGTCAACAATTCCGTCGATTTGAGCGCCTTTGTGGCACTGATTCCTCTGCAAGAAGTCCAGTCCATTGCTGCTCACTATTATCATCATGATGCGGAGTTTCAAAGGTCGTACGCCTTTCTCGCCAGCAGCGATTTCGCGGACATCAAGCGGAAAATACTTCAGCTGCCGGAAGTGCTGGAGTTCACCAACTATCTGGGTAACAATGGACTAGATGTCGTCAAGGTGATGCATTCGGTGGCAGGAGTGTTCAAACCGTCCATTCTGAGCTCCGCAGCGGTCAACGGTGCGTATGAGTAATGATTTTTGTCTTAGTAAAGATTTCCTTTAATTATTGGTATTTATATCCATAAAATCCACAAAATAGAAAGTGAATATTAAATAAGCATTTATTTATTGTCTGAAATCTACTGAGAACTATTTCTGCATTTTTCAGAGCCAACAAAAGTCACAACCACCGAAGATGGCAGCTCCGCCGCTGGAGAAGCACAAACTGGCCTCCATGGAATGGTGGAGAGGGTCCTGGAAATTCTGCCGCAGGACCAACTGTACGCCCTATTCTTCGACGAGTTTGAGAGCAACAAACAGTTCGCAGCATTTGTCGACAGCATTAGCAGTCCAAAGTTTGCCAAGATTTTGAGCGGTTTGCAGGTGAGCTGGGAAATCCCGGGTCCACCATGTGGTAATTGTATTTCAGCCAGGGGACACGCTTTGT

GCTACGCGTATGTTTGGGGAAAATTGTTTAAAGGAATTCCATTCGATTGCCAGCTCCATAATAATTTCCAAGAGGGACGTGAGAAAAATTCCCGATTGCTTTATTGAGTTTTGGCCAAAAAGGTATAGGTTAAAAGTGGGCAACCTTTATATGCGATTCCGTATACAAATCAACTTCCGTTGATGCCTACGAATTTTCAAGTGCCACCGCCTTTTTCTTTTTATGCTTTTTTTTCGGCCACATTTCCCAGTGTTTTTCGTTTCGCCTCGCTTCTAATTGGCAATTGAGCTGGCCTACGTGAGGCACATATTTATGAAATCAAATCGACAGCCAGCAGGCAATTTATATTTATTTTTCCCAGCCCCATAACCATATATATTTATCAGCCATTCCGATGGAGAGTGTTTCTGATAATTGATTTCGCCGCACTGATTCACGTCTCATTTTCATCATTTCAGAACTCAATGCCGTTGCGAAATCTACTATTTGTCCTGCACAATAACAGTATCTATGTGGAGCGGATTGTGGAGTCCGTAAAATCGTATCTATCCATTTCCAGTTTTTAGTTTGCCTAAGTCTTAGGGGCTTGTTTTAAGCTCGCTGTGGCAAATAAACTGTATGAATTAGACAAACTTATTCAAGTATATGACCTTTATATCATTTCATCCTAATTTTGTTGACTAACAATTTGGTAAGTATGTTATCACTGAGTGTTCTGTTTATCTGCCACTTTTTGCCCACCACGAGCATATGAAATTGGGCTCCCCATTTAGTGTGTAATAAACGGAAGGGGAATTCTTTAAAGACATTCGTTTGTCCTGTTGGGTGGGAAATCGATGGG

 

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