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CRIMIC CR01470

Gene cup

CG11181

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6667759..6667775	26F6	FBti0210394	86408

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CAACGCGGCAGGTTGACATTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGTGAGATGAGCATGGTT	GGCTTGTCCCGTGAATCTGA	GCGTTGCGATTACAGCTCCT	GACGTGGGTCCACAGCTAAA

 

Amplicon 5'

Amplicon 3'

TGCGTGAGATGAGCATGGTTTCGAAGGGCCAAGTGGCCCAGAAGGGCACCACCACCAACCAGTCGCGATTCTCAATGACAATCCGCTCCTGGCGCTGCAGCTCACGCTCCACATAGTCGGCCAGCATGGGCCGCTCGTTGGTGGCGTAGTAGGCACGCAGACGCTCCTGCTTCAGTTGCGGTTCCGTCGGCAGAAACGAGCATGACCAGATCTGGCAGTGCGGATGGGGATTGGAACACCCCATGGCGGCTCCCTTGTTCTCGAATATCTGCACCCACGCGTACTTGGCGCTAAGCTCGTTGAACTGGCTGATCCACCTAGATAGGCAGTTAGATAAAAAGTGGGTTTATTATTTTGAGTATCTTCTGATATAAACCATAAATCCTCCACTTACTCGTCGATAACAACGACGATTTCCGCTGCACTCATCGTGGGCAAAGTCAGATTCGATTTGGGATGGAAGCACATCACTCGGCAGTTGCCACGGGCGGGAGCTATCTGGAACAGTGGATCATCGTTGTTGGGCGGAACGGGCACCACCTCCACCAGGGCCGGGAAGTCGTTCTCAAACACATAGGTGCTCTCATATTCCGGAGTTTGCTGATCATTTACAAAATATACACGTGAGAATGCTTAAAACATTTGCGAGGATTTAGTTTGGTCGCTTACGATTCCATTGGGTCTGGTGACGCCAGGGCACAGAGGATTGGTGGGATCGAATTCGGGCAGCTCGTTCTTCTGCGCCTTCTCCTGCTGACCCGACCAAGGGCGTTGGGTGCGATGTGGGCACACTAGGACCCATTGGCCGTTCAACGGATTTAGACGTCGGTGCGGGTGCTCTGCAATGACATTCGATTTTAATTAAGATTGAGCAGCGATAAACCAGACCATTGATTTGGAACTAATTGTTGATCAACATATGACCATTCGCCATAGAAACTGAATCAAATTGGCATGGTGGAACTTCTTTTAAACCTACCACTGGCCACAAACTGCATCGTGTCGAAAGACTGGCGCTCCGTATCACTCCGATCGGATTATGACTACTTCAGATTCACGGGACAAGCC

GCGTTGCGATTACAGCTCCTAATGCTGCTGATATTGGTGTTCCGATTGTGATTCTGCTTTTGCTTCTGAATCCGATTCCAGTTGAGCTGTTTTTCGCTTAGCAAAGCGCGCTTCTTCAGCTACATTCCTCTCTTTTCTCTCTTCGGATTATTCCGCTCACTCTGCCGGCGAGCGTTTAGTTTGAGTTTGGCACGCATTCCGCCTTTCAGTGGAAGAATTTTCCATCAGCCTTATCTCTCCCACTCAGCTGGGCGTTCTGATATGGGTGTGTAAACAGTGTGAGCGCGATTGTGTGTGCCGCTAGTGATGCGCGGTGGTCGATAAGACGTCCATAGAGATGGCTGTTTAATGTTTAAATAAGTATAGTTATATTTATATTTTATGCAGTATCTGTTTGCCTAATTATTGTGATTGGAACAAACTGTTTCCTAAAGTCTATTCAGCTATATTGCAATCACTGTAATCTAATTTCTTTAAAACTTATTAGCTAAGTAAGAGGTATATGATAGCCGAGAGAATTTTAAAGTTTTATGGAAAAATCTACAGATATTCGAATAATTATTTCAGGATCCGGCCATTGTTAACCAAAGACGCATTGGCAGCGGTCGCTTAAACCATTCCAAGTGGGGTTACAACGACGAAGACTACCACTCGTACCACAATGGCAAGTCGCAGCATATGGAGGAGGTCAACTCGAAGAACAGCAAAAACATGACGGTGCTGCAGTTCTTCGACAACGGCGAGATCAGCAGTCAGCCTCAAAGACGGCCGAACACTCCTGTGATGGGGATGTCTATCAATCGATCCGAGAACGACACCCTGCACTCGAACGAGTCCAGTGAAGATTTGAGTCGCGCCAACGAGAACTATGTAAAGCGCGTAATGTCTGGATTCCTGGTTGTATCGAAGCCCAAGTCACGAGATGTCGAGGATAGACATCACCGGCGGTACAGGAACCAGAACGAGGAGCCTGAGTGGTTTAGCTGTGGACCCACGTC

 

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