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CRIMIC CR01471

Gene CG34136

CG34136

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:21098046..21098060	39A1	FBti0210395	86409

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK

Images

Construct Design Sequences

sgRNA sequence	GAAGGACTACCCCAATTTCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTCGTTTCCCTACTGTGCC	GTCCTTCCAAAGATCATTGCGG	AGGAGTCAAGTTCAGCTGAGTG	CAGGCTTCCGGTGAATCCTT

 

Amplicon 5'

Amplicon 3'

TGTCGTTTCCCTACTGTGCCTTGCATTCTTCGCTTGGGCAAAAGCTGGACCTGTGCCAATTGTGAATGAGGTGAGCGGAAGACAGGGGGCCGACGGGGCACGGCGAAAACGGAGTAGTACAGCAGGGGGCACGAATTTGGGGCAATGACTTTACTCGCATTCCAAAATAAAATGGATGCCAGCTGCACTCTGCAGGAAGTGCAAACTCGCTAATTAAAAGTGGGCGTCGAGTACCGCGAGAACTACAGAGTGCAGAGTGCACTCTTAATTAAAAGATTGCGTTTGAACTCAGAACAGCGTGGGGAACAAATCCATAACAAGAAAACATTATATGGCAAGTGAAGGTTCAGCAACATAAATAATTTACGTAACATCTGCCACACATTTCGGTGAAACACCTACTTCAAAACTAAACACTTGTATTTCAAAGTTATTACACGATTAAAAACCCGAAACTGCAACCGAGGAAAATGATTAGAATATTTTCTGCTACAACTAATATCATGGGCCTATCATCAATGTATCTAACTAAATATTCTTGTATGCCATCTGGAAAACTTGCCAGCATATTAGTCATTAGTGTGCCTGGTTGTAAAGGATAACAACCAGGATTTTCGTGCACGTTTGTAGCGTGTGTAATTAATCTTAAGTTCTACATACATATAGGAGAGAAGACTTTTTATTTTTAAGACTCAACGAGACAGCCGTTTATATAATATTCCATATGAAAACGATCCCTTATCAATTCCCATGAAGACTTGACCCTTGATGGAATAAACAAAGTAATTAATGTATTATAAAAATAACAACTGATTACCGCAATGATCTTTGGAAGGAC

AGGAGTCAAGTTCAGCTGAGTGTTTAAATACTAACCAATATTTTTGTCTAATCATTATTTTAGAATTCTGGGTATCATGTGATAGAACGTCTTATGTGAAGATCACCTCCGGGAGATCTTTGCAAAAACCCTAACGGCAAGAGCTTAAATCTAATATTGTTCAGTTGCTAGGCCGCTTTAACTCACACGATTTACACAACACACAATTGAAAGGCGGTTCCAAAAATAAAACGCGCTTTAAGAAAACTACGTTATAAATCTCAATGGAAATTATTTAAATTAAGTTGAACACTGTTTGGGCGCAGATAACAAAGCACAAAAGAAAACCGCAACTAAAAAACAAAAGGCACAAAAGTTTGGTGACTCGTGGCGAAACAATTTGACGTCATATTGAGGGAAAACAACATGTAAATGCCATAGAAACCCTTTCCGTTGTTGGTGCGTTTTCATAATATTATACCGTTCTTTAAAACACAATATTTGCCAAGATTAACTATATTTCCCCCATATAACGACTCTTCCAGCACCAACAATTGATGCCGAAAGTGCCTCAATGGCACTGCCTGCGCTACTTTAAGCATGATGTCCTGATGATGCGCCGCTGTCGCCACTTGCGAGTCCCTACAGCGCCACGACTGGGCGATGTCCTCAAGCGAAAGAAGAAATAGTATAAGGCCCACAGACAATCGTCCACAATACTCAAAACGACTGAACCAGAAGATCAATTAAAAGAACATTTTTTAAAGGATTTCTAACCAGACCAAAGCTAAATCAAATATATACTACCAAATATACAATTTCTGGAACTTAGTATAAGTCTAACCAATTGTACTTCATAGTTGAGTAAGATTTCAATAAAACGACTAAATATATAAAATTGCAAATTTGACTGGGAAGATGGAAGCAAACAATGAGGATAATAAAAATGTAAGTATACTAAACTACGGCATTGTAGGCATTTTAAAATTTAAACTTACCGCGTTAACGGCACTGTGTTCTTCCCGTCCCACGATCAGTGGTCCGTTTTTCTCCAAGGATTCACCGGAAGCCTG

 

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