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CRIMIC CR01473

Gene CG30069

CG30069

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14187404..14187419	50E2	FBti0210397	86411

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACCCACGACGAGGTGCTCGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCGATGTGGTGGAGATCGC	TTGGGAGAGTCGAAGGAACG	CTCGTCGTGGGTGTGTGTTT	TGTCTTGGTGTCGCACTTGA

 

Amplicon 5'

Amplicon 3'

ATCGATGTGGTGGAGATCGCAACGCCAACGCTCTCCGTCTCCGCCAGCGGGGGCAGTGTTTCGAAAATGTTCACGTCCTCGTCGATGTCTTCCAGTCAACAGCAGCAGCAGGCCAGTAGCTCCAGCTCGTACTTTGAAGCCACAACCAGTTCCAGCCGGAGCGCTAACGAAACGCTGATTGCCAGCGAGAGAGCCGACACGGCTACCGGTATGACATCATCCGGGCCGAGGAAACAGGTTGGCTTCGATACAGACACCAAGACAGTGACAAACGCCACCACCAGCAACAGCAACAGCAGCAGCAGCAGCACAAGCACAAACATCAACATCAACACCAGCGGCAGCCGCAGCAGCAATTTGAGCGAATCTCAGAGCGTGCCAAAGCTGCCAATGAGCGGAACAGCTGATGCTCCCGCATCGGTGACGTCGCAAGGCTACCCCGAACCGGTTGCCGCGTCGTCTGGCGCGACCACAACAAAGGCGACGTCATCGACGCGACGCAACGATAAACTGGAAGTGGCCAGTGCTAAAACAGCTGTTGATAGAGCTACGTCATCGTCGTCGATGGTCGACCAAAGGTCCACCAAAGAGGTTAGCGAGAGTTCAGTCTTGAAAAGCTCCACCAGCAGCAAGCAGTCAAAGTCGTCGTCCAAGAAAGAGGTGTACGATGTGAAGACCAAAACCTGGACGGAGTACAGCGATGGCAGTTCTCATGGCCCGAGTAAGACCCGGCCGACCTTTGAGCGTTATGTCAGCAAGGAATCCGATGGCACATCCAAAGTGACGTACAAGAAGAAAATCTACGACAGACGCAACAATCGGTGGCGCGTGGTGGACGAACGGACGGTGGATAGCACCGCCGACACGGGCTATCCCGAAATAGTCGATGATGTCATTAACACAACAAGAACCACATACACCACCAAGGTGTACGACACGAAGCTCGGCAAGTGGACTGTTGTGGACGAGAAGTCGTTCACTGATACGAAAGCTTTTGTGCCCAATGACATTGCTCGTGAAATCGAGAAAGATAATACCGATGTTGCAAACATAACGACTACAACGGAGGTGACGAAGGTAAGTCGGTGATTGTGCGTGGCAGCGTGTGGGCGTTCCTTCGACTCTCCCAA

CTCGTCGTGGGTGTGTGTTTAGTTTAAATTCTAAGTCATTTTTTTTGTTGTTGAATTAGATTTTCGATGCGAGCATCAACGACTGGCGCGTTCTGGACGAGAAGGTGCACACGGATGTTATTGAGAAGATCGTAGAGGCTCCCAAGAAGACCATTTACGTGGACGAGTTTGTGGAGATTGAGAAGAACACATCGATATCGGAGAACAACGAAAACATAACACTGAACTTGAAGGAGACATCGAAACACAAAAATATTACCGAAAATATTTATGATGAAATCGATTATGTGCGCACTGATAAGCAACGCCTGAACGACTCAAGAACAGTGAGTAGATTAGTTAAGCAAAGAACATAAATATTTGTGTTTAGAAAAACGTTGTTGAATTAATAACAGCTTAAAATATATTGCTTACTAATTATTTGTTTTGTGCTTTCATCATTAGCGTGGAGTTGCAAAAAACAAGTCAACAACACATAGTGAAAGATGCATTTGTGAAATCTGCACATGCGGGTAAGATTCAGTGTACGCGTACATAATCTGATATACTAATTGCCTTCATATTTTAGGCGGCACCATTGCTCAAGCAGTACAACGAAGTCTACGGAAAATACGGTTATTCAAACTGAAGGTAAGTGTAATTGTATAAATAAATTATAAAAAAGTTAACCCCTTGGAGTTTCCACAGATAACGTTGACGAAGCATATACAAGGATACGTACAAATACATGGTCGAAAGAAGATAATGGAAACATTCCCAAGCAAAACGAAGACATTCTAGTGGACTATATAGTCATTAAGAAGCCAGAAGACAACTTAAGACCTGAGGGAGACTTTATAGTTCCTCCGAAGGAGCCATACAAGCCGGGAGAAAAACGTGAAAAGATTGTGCATACCGACAACCTTCGTACAGAAGGAGAAATGACTTTTGTGGAGAAGGAGGAATACCAATATACAGTTCGTCCGGGATACGTTAAGCCTACAGACAATCTAAAGCCGGAAGGCGAGTTTTACAGTCCAGAAAAACCTAAGTATCAACCAGGAGATCGGCCATCTCAAGTGCGACACCAAGACA

 

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