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CRIMIC CR01474

Gene Khc

CG7765

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16270813..16270830	53A4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACTCATTGGCGCGCGGACAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCAGGTTCACCTCCATCG	ATGAGTCATGCACGCAAAGC	GAGTGGAAGTGGAGCGGG	CCCAACCCAATTTTCGCGAG

 

Amplicon 5'

Amplicon 3'

CTCCAGGTTCACCTCCATCGCGTAGATGTGATTGAAAATGTCGTTGACGATACGTGGTATGATACCCTGTTTTACGGAGTCCCCGATCACGCCCTCCATCGTATGCGTTTTTCCGGAGGACGTCTGACCATATGCGAATATCGTTCCATTGTACCCGGCCAGGACATCCGTAACAATGGACTTGGCCGCCTCATTGTAGACCTTCTCCTGGGATGCATTCGGTTTGAAGACCTTGTCGAACAAATACACCTTGCCCTGCAGACGATGGAGATTAGGTTTAGTATAATGTGTTTTTTTCTTTAGTACATAGCAGCCAGTTACATTAGCTATAAACTGGTGTCAGTTGCGGTGAAATTTCTTTCGGAGATTCATTCCCCCTGTCCCCGATCTTTTCCCGCTGACTGCCTTTGAATCATTTTCAACGCATGGCCGCGAAGCAGATTGTAATTATGAGCATTAAGACATGTTAAGTGGAAGGGGGAAGAATAGTGAATAACGAGCGCCAGAAGAAAGCCACAGCGAAAGAGAGATAGATAGAGTTAGTCAGAGAGAGAGAGAGAGCGTAATGATAATGTCTGTGTATGGAGGCACTATATTAGTGCTGACTTTTTTGGAACTGGTTCAATTGCCCAAGCTTTACCAGGAATATGACGGATTACACCTGGCGGAAAAGCGGGAAATTCAGCATAGCATACAAAGCAATAACATTTCATGTGGCATAAAAGCAATACAGCCAAATTCCATTGCCTCCAAAGTTGCTCTTATTGTTTTTCCTCATATTTTTTTTTTTTGTATGCTTTGCGTGCATGACTCAT

GAGTGGAAGTGGAGCGGGGGCTACCGTCAATGTGTGTATGTGTTTGTGTGGAAATGTAAGTGAGGTAAGCACTGAAGGCGGACTGGAAACTAGAAAAGCGCATATTTATGCGGCCCAAAAGAATGGAACACCCGCTTTTAGCAGAAAACCGTCTAGTGGGTGGCATTTCTCCACCCCGTCATCGCGCTTCTTTCGATTTACACACCCTATTCTTTTTCGACTGGGGGAAAATCAATTTGGTGCAAAGCAATATTGATTTGGAGTAAAGTTAGTTGGTTTCATCATATACATGTATATGTACATCTAGTTTTGTTTTGTGCAATTATTCATGGGTAACTTTAGTGCCTCTCTCTTTTTATCGCCTGGCATTCCAATTGGTCGGCAATTTTGGGGAAGGGTAATGTACGCTAATAGGCATGACGTCATGGCGCGGCACGCCGAGATTAATGCATAACACATAGGGAAAAGGCACTGGGGGCTGGCGAAAACTATAGATCTGGAACACATACCGCTATGGATATGCAGTTCTCCTCCACATTGTTGGGGAACTTGACCACGAACTTGGAGCCGGCCTTCTCTTCGCTGTCGTTCAGCGGTCGGAATCGGCAGACCACTTTGATGCTGTCCTCGGCGGGAATCTCTCGTTCCGCGGACATTGCTTACAGGGCGGAGATAGATCTTTCTGGCGGAGATTGTTGGATTTCTGCAGCTCTGGGCGCGACCTCTGCGCATGCAGCTCACTGTTTACGTGGATAATCAACGGACTCGCTATCGTCCGCACTATCTGCTGTTCACTGTTTGCTTGTGCCGATTCTGGTACTCCGTTTGGCCCCTTTTGTTCCGATTCTCTGCTGATTCCGACACACTCGCGAAAATTGGGTTGGG

 

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