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CRIMIC CR01476

Gene CG15705

CG15705

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16281351..16281358	53A5	FBti0210398	86412

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK (weak)

Images

Construct Design Sequences

sgRNA sequence	CCAAATGCATCAGAAATACGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTACTTGGGAGTGGTGGAGC	CGTGGTGCCCGAGTATCG	TGATGCATTTGGACAAGCCTATT	CTGTCCGTCGTCTCCCAAAA

 

Amplicon 5'

Amplicon 3'

GTACTTGGGAGTGGTGGAGCACCTGGTGGATGTGGTGGCCTACTGCATGTGCCGTGTCCTGGAGCTGTCCCTGTTCGCCTGCATGATGGTCTGCGGCTCCATGAGAGCCAAGCTGACCCACGGACCCACCAACGAGCTGGAGCAATGACCAGGACACCAGGAGTGCTGCTAAAAGGATGCGCGCGTTTGTGTGTTTGAGTGTGTTTCGACTAAAATTTAATAAAGGCAACTTTTCTAGTGGCGAATAAATTACAACTCATATGTGCTTGTAAACGATTGTCTTTTCCTCCCGTTGGCTGGCCCTTTGGAATTGAGCCATAACTCAAGATGGCCCACGGCAAGGATGTGGCCAAAGGGACTGGCTGTTTATCCTTCAGCTTCCATTGTTGGATTTTGTTTTTGTGCGGCGTACTCCGTTCATGGTACTTATTAGCCCTGAAGGGGATTATATGGGCAGTAATACAATGCCCATTTGCATGATGATAAAGTATCGACTGGGGGGGCCTTCCATGCCCACATGCTGGGCAATTGTGTGTTGGGGCTTTTGTTTTTGGCGCCTGCAAATATGCTCCTTTTTATTTGGGGAGTAGCCCCCATCCATTTCATAGGATAGTCCTGCTTCGGTATTTATGTAGTTGTTTACAGCCTCCGGCTTGGAACATAAATCCATTCAAATTGTACGTGCAAATGGGAGGACATCATGTTATATTACACACACCCGCAGCAGGCACTTCGAATTTGAGTTTTGAGCAAGTACAATTCATGTTTTGATACATTTTTGATAGCTGAGCAACAAAATCATACCAAATTCCCCTCTGAACATGGACAGCCCAGTAAAACAGCGCCTAAAACCCAAGAAGCTGGACAAACATCCAGGAACTCAGAAGGTCGAGTTCAAAAAGGATATCATCGATGAGGTAGCGGATCAGTCGGTTGAATACGATTACATGAAGTATCCCAGCAATAAAGAGAAGGCAAAACTCCTGAAAGGCGTTAAACCATCGAAAAGTGTCACATTCAAGACGGTGGTGGAGCTGGTGACCTATTCGGAAAACTGGAAGATGAAGATGAGCGAGAGCAGGCTGCGCTCGGAGGATGAGCAGGTGTGGTTACGGATGCTAAAGTCCACTTTCCGATACTCGGGCACCACG

TGATGCATTTGGACAAGCCTATTAAAAATTTTAGTTTTACTAGAACAATTTAAATAAAATACTACTACTACTTTTTGATACGTTTTCCAATGCATCAGACTTATTCTTATTTTCAGTTTCTTATATTACAATCTAATCATTCTTTTAATCTTAAACTTTAGCTGAAGAGCTCCCAAAAATATCGCAACTACTGACAACTTTCCTTAAAATTATTGTTCGTTGTAAGCTAATAAATTGTTATGTGTTGATAAAAAAATATTTCGAGAAAAACTAAAAATAGTTGGTTTTTATTTTGGAAAAATAATGGTCACAGACAATTTAATGAGAGTTTTTTTTCCCCAATATCATATATTTTATGGCAGGATCTTTATTTACCCAATGCTCGGCATCGCAGCTGGTTGATGATGTTGCAAATGACATACTCGCCGGTATCCTGGCCACCGAACTCCTTGGTGCGAATGCCTTCGTCCATGGTGCGCTTCAGCTCGCACCACAGTGCATCGGCGCACTTATCGTGGCCCAGATGCTGGAAGAGATCCACAATGGTGGACACGATGCCGTATGCACTTAGATTGGCATAATTGGTCAGCGATAGTTTCGTCTGGAGCGGTTTGAAAACGGCATGGTGATCTCCGATCTCCACCGCACTGAAGAGATTAGCCCCGCCACAGACTCCACTGCAGATGGCGGATAGGAATGTGGCATAGCGTTCGCTGGCGAAGAGGCAATCGAAGTAGGTGGGCTCCGTCATCAGCCGGCTGATGGCCTCCTCCACCTCCATGAGCTCCAGCTCCAGGCAATCCTTGTAGTGGCAGTGCAAGCGCTGTGCGGCTTCCACCAGTGAGCCATCGCTAATGGGCCACTCCTTGGCCTTGTTGATCAGCGTCACCCGTTTCCGGCCCGCCTTTGCAGCCGCCTTAAAGGCGTACCGCAGATACTTGTCATTGCCTTTTTGGGAGACGACGGACAG

 

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