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CRIMIC CR01478

Gene phyl

CG10108

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14429622..14429655	51A2	FBti0210399	86413

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK

Images

Construct Design Sequences

sgRNA sequence	GGTGATCGGTACGAGGGTCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCTTCGTCCTTTCCTGCT	CCTTTATCTAATTTCTCGGCGCA	TCGACCAGGATGGCGATAGA	TGGTGGTTGAACCCTTGGAG

 

Amplicon 5'

Amplicon 3'

GAGCTTCGTCCTTTCCTGCTGCAGCACACTGTTGCCCATGTGCGAGGGACTTTGGCTCTGGCTTTCGGCCTCATTGCTGTTGACCGCCTGCAGGGAGTGCCAGTTGATCAGCCAGTTGTTGCAGCTAAAGCAGAGGAATTTGTCCTCGTTCAGGGGCTGAAAGTAGAGATATATAGGGAATAGATGAGTACGGGTAGTAGAAGTAGTTTTATCTAGACTATAAAGTTATTGCTAATCATAATATACTAATCATAACTATTCAAACTCATTTGGTCCTTCAATTAATTTAATGGCTGAGTAATAACCCATTTTATAAATTGCTTACTGCCTTAGACTGATATGTAGCATTCCAAGTAAAGGATAAAAAGCCATTAGAAAGAACCGAAGGCGGTTACATTGTAACTTGCAGAGCCACGAAGGCCAATCATTTATTGATTATAGCCATGAAACCGTTAAGGTCATCAGGAACCAACCTTAGAATGAAAATTGACCCAAATCCGATTGCGTGACTCGCAATCTGAAGCCAGGCATAACCCACAGAATAAACCTCTAGAAAATTACGATACGCCTATGCACTTAGTGATCGCTCGCGACACCCGACCACTACCACCACCACTACCACTAATGAGCATCCAGCCAGAGGTGCAAAAAAAAAAAAAAGACCATAACAATTCCGAAAAACAACTGCCAGACGACCCGGGCATATTAATTACCCAGCCAGACCTCAGCATTCCTCGGGTATCAATAATTTCGTGCACCTGCCAGCAAAAACTAAGCGCCCAGCGGTAGCAGCTGGCCCAAAAGGTACGGAAAACCAGTAACCAGTAACAAGCCAAAGGGACATTCGCTACTTAGTTCGAGCCTCGAAGGAAACTCGTTTTCGGCCAAGAGCCACGCGCCCTATCCATATATATAAAAAAAAAAAACACATAATGCCGAAGTGGAACCGAAGCGGGAACTGGGTTAAAACCCCAACTATGACGACCATTAGGCACGCGCATGCGCGACAGCTGCGCCGAGAAATTAGATAAAGG

TCGACCAGGATGGCGATAGAGGAGGAGGAGGTGTGCAGGGTAGAAAGGATCAGGAGCGATTGTGGCTGGCTGTTTACCTTTGGGTTCGGCGCTGAAAAGGTGTGCTCCAAGGGCCATGTGTAAACGGTATAATAATGCAAATTGCAGGCCAGGTGCAAAGCAGACAGAAAGCCACACAAACACACACACACACACGCACACGCACGCACGCCGATCGCAGATGCACTGCGAGAAAAAGGTGTGCACTCCTAAGATCAGTTAAATATGGGCACATGAAAAATATGTTTAAAAGTACTTCTATTAGTTTGAAAACAACTCTTCGTGCTTCTGTTAATATAGCTGCATATTTACTCAATTTGTAATGTTCCTTTTTTCTAAGTGCATAAGAGTGGGAGGTGGGGGGGCGCAGTCTGATAAGGTAATTGCAACTGGACTTTATGGCACTGTCATTCAGAACTCGAGCTTATAGAGAACCCGGCGAGGGTGTTGTTCCTGTGGGGGAGATCGGTTGCCAGGCTACGATCACGGCAGGTGGTGTATGCCCACCGTATAGGGTTTCGCAAAAACGGTTCAAACGAAATTCTAACGACGTCCGGAGGGGGTGAATATATATATATTCTATGTGCATAGAGGGTGAGCTTCTAGTGTCTGATATTGCGGGGTTAGCTGAGGGTTTTTGTTATTATGAAATCGCATTGCGTGCGTCTCGAGCATTTCGTAATTAAATTGATTGCTATACACACAATACTAGTTAAGCACAATGCTTCAATTAATCTAAGACCGAAACATATATTCCGAAAATATGTGGAAATACAATCTACCAATATCTACCCAATATTTTAACAACCTATGTTAGCAACCACTTAATATTGTTTATGTTTTTATAGGACTTCGTTTTAAGAACTCTCATTCTGACTTTGTAATCCAATTGAACGGAAACCTTAGTAAAAACTATTTCCCAAAAATACCAAACTAAACGAAGACCTCTAAATGGAAATCTTGTCTAGCTTGGCACTTCTGGGACTTCGGCTGAAAATTCGGGCATTTGAAATCACCTTTGTAAACGCTATTCCAAGGGTAGATCCTTTGTTTGGATTTGCGCAGCTTAAGAAACCGGAATTTCTATCCAAAGATATTTGGCATTCTCCAAGGGTTCAACCACCA

 

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