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CRIMIC CR01482

Gene CG10188

CG10188

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19507402..19507421	37E5	FBti0210400	86414

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ATATTTGTATACCTGCAACATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCAGCAATCCTCTCGAAGT	CATGGCTGGCCCTGTTGATT	CTGAACTACTATTGCATTGCACA	AACAAGGTGTTTTCGGGGGT

 

Amplicon 5'

Amplicon 3'

GGCAGCAATCCTCTCGAAGTCACGGGCCAGCTCCGCCTCGTTTAGCGAATGGGTCGAAATACCTGCGAGTAGTCAAAGTTAGAGAGTCGGTCCGTTAGCAATAACTAATCGCAAGGGGTTTCTGATAGCTTTGGTCGGAGCTACCAATACCAAAACACTATATGGGCTTACAGATAAGGGGGTTTTGTGGAATTCCACATCGAAACGAAGCTAACTTGGTAGTAGCCCCTACTCTTTGGTTGAGTGGCATATTATTTTGAGCAATCAATTGGCAAAATTTATTGACTTTATTAACGATATCTTATCATACATATTTTCCCATTTTTTTTCCGCGAGTCCCACGGTCACACCTCTACAATATTCTACATTCTACAATAATTTAAGATTAGGGCTTGTAGTTTCTAGTGAATCTGCTTGGAACAGAGAAGTTATTCTGTCGAAAGGTCATAACTTTTCACTTGGAGCCATTTAAGTTTAGTACGTTACCACGGCACCATATTTGAAAGCTATTGAAATCGGATTGTAAAGCTAAATGGGAAGAATTTTCATTATACTGGAGGCATAATTTACCATACGATGCGTACATAAGTACAAGCGAATGTTTCATTGCTCACTAGGAGAATACGAGATAGAAAATTTTCAAAGAGTACCCGGATGCGTCCTTTAAGTTCAGATAACTTAAAATCCTTTTTAGAAGATCAACAGATAAACCTAATAAATCAAGTTTGCTTACTAGAAGAGAATTATTTATAAAGACAAATGCTTTACTAAAGTCAGTGTAGATGACACCTGTTTGAATATTATTTCTGAAGCCCTGTATTACGAAGGAAGTTAGCTCCAAAAGGTTATTGGTTGTTTATCTGCGTTTCTGCTGACATGGTGATATAATTGACCTACAAAGGCGCTGCAAATGAGTAGTGATAACATTTTCAAAAACCTTTGGAATAGCCGACAATTTAGAGATTCCTCTGTAAAATCCATTTCCGACTTAATTTTGAATGCATTTTTGTTTAGTATTATTTTAAACTTTCAACACCTAAACACTTTTGTCTAAATAATTAAATAATATTGGCAAATTTACCTAATCAACAGGGCCAGCCATG

CTGAACTACTATTGCATTGCACACTTATGGCTTTGCCAACCCACCCCCGAAACCTTAGTGCATATCTACCTCCAGTGCTGTTCCTGGCCGACCTTCGTCGCTGCTCGGTGACGCGAATGGTTTCCTGCTCCTCGCTGTCCAGGCTGGATAGGCTGACGCTCTTGTTGGTGCAGTCGCCAGCGCCGCTGCCATCGTCCACGGCTGTCCAGGATTTCCGGCGGTCTGTGTGAAGAAACTGGCACTGCTGCGGTGCCGTGGAAGACCACATGGCGTAGTTGGAGCCACCGGACGCGGGACCCGTGCCCGTCCCCGTAAGATCGGGCAGCGAAGTACTCAGTATAGCTGCGCTGAGCAGTCCATGATTGGCCAAGCTGCCGTCCTGCATGTGCTGGCTAGGCGAGGAGGAGTTCTTCATCTGGACGACCGTTTCGCGGATGTTCTGCAGCTGGCTCAGCGTGTCCTCGAATATGTTGTTGTACTTGGAGGCCAGGGCGGGACTGTGTGGCGTCACATTTATGGTGGGTATCATGTTGTTGTTATTGATGGTGTTGCTGGCAGGATTAGATGCTGGTGGCGGAGCAATGGGGGCGCGCGCTGGACCAGGAACGGGTGCGTTTATTACGGGTATGCAATAGTTCTGGCTTTCGATGGAGGCGCCTCGGTTGGTAGAGCCACCATTAACCAGCGCTTCCGAGGACTCGCTGCAGTTGGAGTTCAGAAAATCGGTGACCACATCATTGTCGCTGTCCTCGCCCGCGTTGTCCATAATAGGGTCTACTGAATTCTCTGCGTTTTGGGCCAATTTCCTTTGTTTAGCGACTGTGTTCTATTTTACAGCTGGCTGCCTAGAAAAAGAAAGTTTCAGTATACAACGCACACACACTCAACCGCGACGGGCACCATACATGCATATATGTGTGTACATACGTACATATACACTTGTGTATGCACTTGCTTTTTGCTATTTATTGATTTTCCCGCCACCCCCGAAAACACCTTGTT

 

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