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CRIMIC CR01483

Gene CG11929

CG11929

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:4684270..4684283	25A3	FBti0210401	86415

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band

Images

Construct Design Sequences

sgRNA sequence	ATCATAGGGCAATAGGGTCAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGGGCACAGTCCCTAAATG	CCCTATGATCCCGAGACAGT	GGCGAATTCCAAACGCTGAC	CCACGATTTCACAGAGCCCT

 

Amplicon 5'

Amplicon 3'

ACGGGCACAGTCCCTAAATGCCGGCGATGGTTTTCCGGATAGCGTATGATGGGCCAGTGTATCCCGGTCGAAGATCTCGCAGAGCAGCTTCCTCGTGATCGAGGGTCCGGTCATGTCCCAGTTGATGGCACTTAGGCTAATGCGGGAAACTTCGGTGCCATTGGGACCGATCGTCACCATCGGCGATTCGCCGTCCAGGTCGAATTCGAAACCAGGACACCGCCGTTTCGCATTCGGAGTGGAGGTCACCGGTTTCTTGATACGCTTCCTCTTGTTACCCATTGTCTCTTCTCCGCCGGGCACATAGTCCTCATCGTCATCGTCCAAACCAAGATCTTCGTCGTTTGTTTTCGGCGTCGAGGTGTTGTGCTCATCGGCTCCATCGCGAAACGCGTTAAAAGGCTCCGAACTCTGCTCCTGATCCTGGTCCTGCTCATGAGACTGTTTGTGCTCTGGCTCCTGATCCATTTCCTGGACCTTTTCAATTTCGATGGTGAGGAGATCGAGAGCCTTGCGCTTACTGCGCTCCCATTGCTGGAGAAGCTTCAAACGCGCCTCATTTACCGCCTGGATGGTGTTCCTCATCAGCTGCGAGTACTCCACATCCTGCACCATGTCATCGAAATCCTGATCCGGTGAACGCTGTGTCCTTAGCAGCTTCTCAGCAGCCAGTGTAAGAGTTTCCAGAGTGCTCAGCTTGCCACTATCGGACATTTCGATGTGTCGCATGGCGGAATCGGCAAACTCATTTGCATCTTGAATGCGCCGCACCTTGCAGTCATCGCTCTCCTCCTCCGTAAAGGAGTCATCCGTTCGCAGGCGGGCGATGATCGCCTCCACTTTTTTCTGCGTTTCAGACAACTGAACTGTCTCGGGATCATAGGG

GGCGAATTCCAAACGCTGACGGCGGTTAATCTACAAGGAAATGATTTTTTATTTTAAAATGCTTGGTATTTGCTTCTATTTAACTTACCATTGTGCTGCTTGTTGTCCACTTGCAAACTGAATTACTGCTAATAAGTCAACACCTTATATACCACCAATGCGAAAACCTTTACAGGTAGTTTCTTCCAAATACTGATTAATCCTTTTATTACCCTTTTTAATTTTGCAGATGCTTCCATGGCCGATTCGCTGCCCACCGGGGCGACTGCAACTCGCCTACCTGCCGGAGCAACTTGCAGCTGTCGTTCCATTTCGGGACGCCCGAAACCCGCTGCCATCGCCTCGGAATCAACCCAAACCATAGCTTCCACCGAGGACATGGGCACTCAAACCGTCTTCCAAACGGATCCCAATGAGGATTTCGATTGTGGCCAAGATGCATCCACCCAATCGATGCTTAATATGCATGATGTAGAGACTCAATCGATTCCAGTATCGTCCAAAAACCTTAACAGCATACAAACGCAAACGGACTATGAAGAACCAAAAACTGTCCGTTTTCCGTTGAAAAAATATTCGAAGACTGAGCATGAAGAAAAAAGACCTAGAAAATCGAATACTTCATTGCGCAAATGTTTTTTCAAGCGCCCATTAATCGTAGTCAAAGGCGGCGCTCTTATATCTTCCGATTTTACAGCCAATATCCATCAAAGTAGAATGGAGAACGTATCTGTCACTACCAGAAAAGAAGATAAAGAAACACAATATGAAAACCATTTTGGGACACAATCAAATAGTTCAGACGATCAGCCTTCAGATCCTTCAACGTGTTCAAAAATCCTCGATAGAGTTCAGAAACTGGAAGCCATAATGAAGAGACAGGAGCTTTTTCTAAGGGATCTACAGGGCTCTGTGAAATCGTGG

 

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