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CRIMIC CR01484

Gene CG10631

CG10631

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19754893..19754907	38A2	FBti0210402	86416

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AACACAGTGGCATATGCCCACAACCA	AGCTCTAGAGCCGTCTGATACCATAG

Images

Construct Design Sequences

sgRNA sequence	ATTTCGATGGGCGTGTGGGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCGGAGATAGACCTGCTTC	GGGCTGAATGCAGAGAGACA	ATCGAAATGGCACCGGGAGT	TGACCGGCAGCTATCCCTAT

 

Amplicon 5'

Amplicon 3'

GGCGGAGATAGACCTGCTTCTTAAAGAAGACCTCCTTGCAGCGCTGGCAGATGGGCAGACTTTCATTGCCCCGATCCGATTGTTGTTTGCGACGCAGGAAACTGCTAGTGGTCTAGAGAATAGAAATTATGACTACGGTGAGTTTCGGCAAGGAACCATTATAAACTCACCCTTCCGTAGGACTCATCCTTTACGGTCAGCTCCACTTTCGGACTTAGCATGTCCTCGCTGAATTCGCTGAATTTGCTGAAGTCAGCAAATGGCGTATTGTCGTCTATATAGTTGGACTTGTAGGAATGCTCGTCAATGGGTTCCGCTGTTCAAGAAATATTTGATTACTATTTTTTTTACTTTTAAAACCAAAGTAATGTACATATAATACTTGGTTCAAAGGAATAATAAATATGTCTATAATCTAACTTATTAATGCTGGCGATTAATTCAAAGATTTATATAACCGCGAAATGCTAAAACTATGCGCAGATGAAAATTCTGTATCCACTGTTTGCTGTTTCTATGGTTCCGAATTGTATTTTGTACTCTCCATTGGTTGGTGATAAGAATTAACTGCCCCTACATAAAATTGATAGATAACCGAGTGCTCTGCAGACAAGCAGAAATTTCTTCTATTTACATTATTTATGGTACCAACTAGGCTGTTCGCATTTGAACTATATTAAAATCATTCTTGAAGTAATAGCTATTGGACTCATTGATTGGTCAATTAAAAGTAAAAAGCACCAATATTCATTCGTATGTCCGACTTAGTGTTTATATTTATTAGCAACTTTGCCTTCTGCATCCGGATACTTATGTTGATATCCGGATTACGCGAACATGTTGGAGAGACTGAGAATCATTACCTATGGTATCAATGTTTTGGGCGTCGTACCCAATAATATTTGTCTTTGTCTCTCTGCATTCAGCCC

ATCGAAATGGCACCGGGAGTGCGGGGCGGGACCAATAATTTGTAGGTTAGTCTACGGGAGCAAGTTACATAAGAAGCGACTTGAAAGACAGTGGCCCACCACAACCCGTTCCAAAAAATGTGAGGAGGGAGATGATATGCACACTCACTGGACATACGAGTGCATGCATGCACATGTGTGCGTACGAATCGAACTGAATAGGTTACGCCGGTAGTATAAAGCATCTATCCGAGTACACATTTGCCAAATGGATACATACTGACAACCGTATACCATCTCGCACATACCGAGAGTCGCGCAAACAGAACCAAATTGTACGCCACGGTACGGAACCGAACAGAGCAAGATACAGATACAGAGCCGTAGGTTGACGAGCGGTGGAACCGAATCAGAATCCGAATTCGAATCCGAAAACCATCCGAACGAAATAAGCGCCAAACCTCAGCGAAACTCAACAAACGTTTTATTGATTCGACGCCAAAATGGAGCTGAAACTACGCTACTTATAAGAAAATGGCCCCAAGTTAGTTTGATATATAATGCGTGTATTCTGTATCTGTTTCTATGGCAAAGGCGGATTTTGCGTTTAGGTTTTTCGGACTTTCTTCGGCGTAAAGGGATTTTAGATTTGAATATTTTTACTTCCCCATTCAAGGGGCTTAATAAATCAACATATTTGGTCTTTAATCTGAATCGGCAGCTATGCAAAATTTAGTGTTTCAGTTGCCATAAATTTACATTATTTCGCGCTTGTGTGCTTTAATTTGCATAGTAATTAAAAGAATTACTCTCATTTTTATAATAGCTACTTTCGTCTCCTGGTTCTCTGGGAATCTCTTTAAGTATACACAAAAACCCAACCCACTGTAAAACCGCTTCAAGGAAGTATACCCGGTAGTAAACTTTAAATATGATTACTAACTAATATTAATCGTACTAGATACTCATTTTCGGGAACTTACATTTTATTAATACTTCTGGTGCCGGGGGCGGAGCCATGGGATTCGGGGGCTGCTGGTAGGCCTCCATGGGTTCGCTTTTGATATAGGGATAGCTGCCGGTCA

 

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