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CRIMIC CR01488

Gene put

CG7904

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:14623840..14623848	88C9	FBti0210403	86417

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	TTCCTCTGTAATGCAAGCCGACTTCG	TTCGAAGTCAAAGGCAGCAGCAACAC

Images

Construct Design Sequences

sgRNA sequence	TATCGGTTGGAAGTGCTCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAACGAACCAGCGAACGTG	GCACTTCCAACCGATAATTCCA	GTGGGCAAATAAAGTCGAGCC	CGCCAAACGCATACTCGGTA

 

Amplicon 5'

Amplicon 3'

AAAACGAACCAGCGAACGTGCACAGGAGGGCAGAAATTGCAACACATGCGGCTCCGAAAAGGGGTTGCTGGAGGCTGGGGATGTAGAAAATGATGGGGGAGCGGGCGCCACTTCGCGTCAAGAGTTCGTTAACACGAAAAACGCTGCCTCGGATGCGAGGAGTGGTATTTTGTAGGCGAAGCCTACGCCCTCTTTTTGCAACTTGAAGATGCAGTAAAAATACAAAAAAAAAAAAAAAAAACGAAATAACAAAAACAAACAAAAAATATATGCAAACGGAAAAATAACAAAATTGGGGTAAACACTCCAAAGACAAAACCCCAGTAGCAGCAAACGTTAAACCGAATCTGAATCCGAAACCGGATCCGAACCCACCTTGTGTGGTTGCCTCCGTGGTGCTTTTAATATATTTCTGGTTGGAATTGCACCGCGATCCCTTGCAGCAGCAGAAGTGAATGTTTCCCTGCCGTGGCTCTGCACTGGTCACGCACTCCGTCTGATTGCATTCGTGCATGTCCGTGAAGCAGCCCTTCATCTTGATGCGCAGGATGCCTATGGGAACGGAAAAATTAAAATTAATCAGTATGCCTGAAACAACGTCAACTTCTGCACGGCTCACCCGTTGTCTCGTTGACCGACCAAAGGACATAGCAGCTGGGAAACTTATCCGCCTCCATCTTACAGTGCTCTATCCGTGTTTCACATTGCTGCGTTGTGTTGCACATCTTCTCGTCGAAGTGCTCGCATTCTATGATCCCATGACTTCCGGGCAAAATAGATCCATGGATTCCAATCAGACAGCAGACTAAATGAAGACAATGGGAAAATAGATTAATAATCTTTCTAGTTTTAGATTAGCACTGATCTATATTTAAAGTATATATTTGAGCAGTCCTAAAACATGAGTAAAGACTTTCAAAAAGTTAAAACGTAGTTAAAGATCAAAGTTAATGGAATTATCGGTTGGAAGTGC

GTGGGCAAATAAAGTCGAGCCTATTTGGGGAATAAGACTGAAAGGGAGGGCGCTCAGCGAGCTCTTTGTGGGCGCTATCCTTTGTACACATATCGCATGCCCAGAAGTAGCAGGAGTAGCGTCCTAAGCTGGGGCCTCTGCCATTGAAAGCCACCCCATTTGGGCACGGAATTAGCCCCACTGGCCACACACCAGCCTTTGAGTGGGCATGGCCCCAATGCACGGATATGCAGTGCACAGTGGGCCTGCAGTAAGGTTTCTTGAAAATCTATTTACATTCTTTAATTCGATTTAATCTGGGATCCTCATCCAAATACCGAAATTTTTGTTGATAAAAATTAAAATGATAATTCACTTTTTAAATGTGTAAATACAAAAAATTTAATTTTGAAAACGTTTATTGCAACGAGCCTGCGATATATTGATTGATATTTCTTGACCATTGGCTAAGCAAACTGTTTTCAATCCTTTGAAACACTTTTCGTCCCACTGTCCTTTAGGTAATCGGATCGCTGGCCATGTATGTGTGTGTTCGTAAGCGTGCATGCTTTATAGTAAAGTAGATACATAAGCAGGCACATTATGCACACAGAAAGGGATGGGTTTAAAGTGGCGCTTGTGCGGAAAAAGGAAACAGCAGCAGCAGAAACAACAACAACAACAGCAGCAACAGGGAAGAGAGCGCACTGCGCCACAGAAAGAGAGAGGGCTATGGCGAGCGAAAGAGAGGGAGGCCGGTGGAGCAAAAGGGTGGGAGAAACCAGAACCTGCCATACAACAAGTTCCTAATGCAGCAAAATCCATTCAAAGAAATGCTCAGCGCACACACACATGGATTAAGATTGGGAATTAGGTATCCGATCGATTTATACCGAGTATGCGTTTGGCG

 

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