CR01488

CRIMIC CR01488

Gene put

CG7904

Information

Donor Location Cytology FBti Stock number
pM37-p13R:14623840..1462384888C9 FBti021040386417

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short band TTCCTCTGTAATGCAAGCCGACTTCG TTCGAAGTCAAAGGCAGCAGCAACAC

Images

CR01488-1-1-A.jpgCR01488-1-1.jpgCR01488-1-2-A.jpgCR01488-1-2.jpg

Construct Design Sequences

sgRNA sequence TATCGGTTGGAAGTGCTCAAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AAAACGAACCAGCGAACGTG GCACTTCCAACCGATAATTCCA GTGGGCAAATAAAGTCGAGCC CGCCAAACGCATACTCGGTA
 
Amplicon 5' Amplicon 3'
AAAACGAACCAGCGAACGTGCACAGGAGGGCAGAAATTGCAACACATGCGGCTCCGAAAAGGGGTTGCTGGAGGCTGGGGATGTAGAAAATGATGGGGGAGCGGGCGCCACTTCGCGTCAAGAGTTCGTTAACACGAAAAACGCTGCCTCGGATGCGAGGAGTGGTATTTTGTAGGCGAAGCCTACGCCCTCTTTTTGCAACTTGAAGATGCAGTAAAAATACAAAAAAAAAAAAAAAAAACGAAATAACAAAAACAAACAAAAAATATATGCAAACGGAAAAATAACAAAATTGGGGTAAACACTCCAAAGACAAAACCCCAGTAGCAGCAAACGTTAAACCGAATCTGAATCCGAAACCGGATCCGAACCCACCTTGTGTGGTTGCCTCCGTGGTGCTTTTAATATATTTCTGGTTGGAATTGCACCGCGATCCCTTGCAGCAGCAGAAGTGAATGTTTCCCTGCCGTGGCTCTGCACTGGTCACGCACTCCGTCTGATTGCATTCGTGCATGTCCGTGAAGCAGCCCTTCATCTTGATGCGCAGGATGCCTATGGGAACGGAAAAATTAAAATTAATCAGTATGCCTGAAACAACGTCAACTTCTGCACGGCTCACCCGTTGTCTCGTTGACCGACCAAAGGACATAGCAGCTGGGAAACTTATCCGCCTCCATCTTACAGTGCTCTATCCGTGTTTCACATTGCTGCGTTGTGTTGCACATCTTCTCGTCGAAGTGCTCGCATTCTATGATCCCATGACTTCCGGGCAAAATAGATCCATGGATTCCAATCAGACAGCAGACTAAATGAAGACAATGGGAAAATAGATTAATAATCTTTCTAGTTTTAGATTAGCACTGATCTATATTTAAAGTATATATTTGAGCAGTCCTAAAACATGAGTAAAGACTTTCAAAAAGTTAAAACGTAGTTAAAGATCAAAGTTAATGGAATTATCGGTTGGAAGTGC GTGGGCAAATAAAGTCGAGCCTATTTGGGGAATAAGACTGAAAGGGAGGGCGCTCAGCGAGCTCTTTGTGGGCGCTATCCTTTGTACACATATCGCATGCCCAGAAGTAGCAGGAGTAGCGTCCTAAGCTGGGGCCTCTGCCATTGAAAGCCACCCCATTTGGGCACGGAATTAGCCCCACTGGCCACACACCAGCCTTTGAGTGGGCATGGCCCCAATGCACGGATATGCAGTGCACAGTGGGCCTGCAGTAAGGTTTCTTGAAAATCTATTTACATTCTTTAATTCGATTTAATCTGGGATCCTCATCCAAATACCGAAATTTTTGTTGATAAAAATTAAAATGATAATTCACTTTTTAAATGTGTAAATACAAAAAATTTAATTTTGAAAACGTTTATTGCAACGAGCCTGCGATATATTGATTGATATTTCTTGACCATTGGCTAAGCAAACTGTTTTCAATCCTTTGAAACACTTTTCGTCCCACTGTCCTTTAGGTAATCGGATCGCTGGCCATGTATGTGTGTGTTCGTAAGCGTGCATGCTTTATAGTAAAGTAGATACATAAGCAGGCACATTATGCACACAGAAAGGGATGGGTTTAAAGTGGCGCTTGTGCGGAAAAAGGAAACAGCAGCAGCAGAAACAACAACAACAACAGCAGCAACAGGGAAGAGAGCGCACTGCGCCACAGAAAGAGAGAGGGCTATGGCGAGCGAAAGAGAGGGAGGCCGGTGGAGCAAAAGGGTGGGAGAAACCAGAACCTGCCATACAACAAGTTCCTAATGCAGCAAAATCCATTCAAAGAAATGCTCAGCGCACACACACATGGATTAAGATTGGGAATTAGGTATCCGATCGATTTATACCGAGTATGCGTTTGGCG
 
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