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CRIMIC CR01492

Gene Syx4

CG2715

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2742344..2742364	3B4	FBti0210404	86418

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGTTAAACGTTTCCCCTCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTTGTTCGCTGGCTTCAGA	GAGAGCCGGAGAGAGGGAAA	AGGGGAAACGTTTAACTATGCT	AGAAACGTGGGTGGTTGGTT

 

Amplicon 5'

Amplicon 3'

TCTTGTTCGCTGGCTTCAGAATTAACTAAAAAGTAGATTGTAACGAAGTTCTTAAACAAAATAATGTAATAGTTGCAAAGCATTTAATCAGACTCGTCCATTTTGTATCACACTTACTGATTTTTGTATGCAGTCTCAGATTCTTTTTGACCTTGGTCTCGTAGTTCTGCAGGAATAGTTCGTTTTTGTGCCACAGATTGATGAAAGTCTGGTGGAGTCCATAGAAAAGGGTTCTTTTCATCCTTGCCTCCAAGCTGTAATCGTTTTCCGCCGGCAGATTCGCCTTGAAGTCGTTGAATCGCGTCATTAGCTGATTGCCCAGCCTCAGGTTTTTGTTGTGAAGCTCATCCATCTCGTTCTCTGTGCGAATTGGATTTAGACCGTGATGCAGTGGAAGTATTCACAACTTTAGATTCAAATTCTTACCGTTGAAAGTTCGCAGATTGACAGTTTGGGCCATGCGGTTCATCGTCTCCAAGTTGGCGGCTATCTGTGCGAGTTGTTGGCGAATCTCCGTGTACTGAAAAGGAAGGATACCGTAAAAATCTATTAAAATCGTATATTCACTTCCATTGCATTTCCAGTACATTTCCTTGCTCAGGAGGAAGCAGTGCTTGTATTGCTTTTTTGCTTTGCAAGAGTTGTCTACCGTTGTTTACGTTTACATGGCGACTACTTGCCTTCTCTTCGTCCGCTCTCTTAACCACAATAAAAAACGTATTTAATGAGAAAATGTTTTTTTTTTTTTCTGGACTTATCACGAGTTTTCGCGCTACGCCGGCAGCGATTGAGTAATACAGCTGATTCATAAGTGCACACATGTGTGGGTAAGCCATAAAAAAGAAAAAAAGGGTTTCCCTCTCTCCGGCTCTC

AGGGGAAACGTTTAACTATGCTAGATATTAGCTGCTTTCTGTTGTATGTTCTTGCTTCGCTAAGTATTTATAACTTTCAAGATAGCTATCTTAGGAGTTAAAGTTTCTTCTATTAACTATTTCAATACTCAATAATAGTAAAAACTTGTATGAATTCTGAAGTTGTGTTTTGTATACACTTCGATCCTTAAACTCACCGGATTGAGTATGTCATCCACATTCGAGCCATACTGCGTCATCTTTGAACTGGACCGATCCTTGGGTTCGGTGCTGCTGTTATTGTTGCTGCAGCTGTGAGAGTTTTGGCTAACGACACTGTAGCTATTATTGTTACCGCCGGTGTTGCCAATGATGAACTCCGTGGTTCCACTGTACACGTTCAAAAGCAGTGAACCGTTCGACGACGAGTTCGACGAGTTCGTGCTCAATGAGCGCTGGAATTAGTACAGCAAAAATGAGAAGAAAAAAAAATAAATAAATAAAAATAAAATAGGTCCATGCGATCCTAACGAACTGCAGGGAAAAACTGAGAAAAAACTAAATTGAAACTAACAACTTTGGTTGAGTTTATTTACGTTTGTGTGTTCACAATCTGGCGCGGTGTAAATGAACTATAAAAATGACTTCGGTACTGAAATTCACTTGGTGCTAAAAGTTTGTCGGCGGAAACGCGGGGTTGCCCCGTAAGTGTGATGTGTGGCCACATTATCGTCGTTATCTCTGGCCGATCTGATAAATGCCATAGCGAACACCCACCAAACCCCCTTGGCTCACAAGTCGAAATAGTGCCAAAAGCGCCAACAGAAATGTGAGAGATTGGGCCAATTGTAAAGAAGGTGGCAGCACCTTGGTCAGAATGTGTTTCAACGTGCCGCAAAAAAAAAAAAAACTTTGATTAGGATTACTTTTTACCGAGAAAAGTGGAATCCTTTTCCTGGCAAATACCACCAAATGTATTTCATCGTGTTAAGTAGATTTTATTCGTATTGCGGTTTGAGTGATGCCAAACCACTGTGCTTCAAACTAATTAGCAGTTACCTCATCCCAACCTCCCAACCAACCACCCACGTTTCT

 

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