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CRIMIC CR01493

Gene sti

CG10522

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:12514298..12514304	69C4	FBti0210405	91347

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	long band

Images

Construct Design Sequences

sgRNA sequence	GACCCGAGGAAGTAAAAATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTCCTCGCAATGGGAGAGA	ACTTCCTCGGGTCAGAGAATC	TGTGGCTTCTGAGTTCCATT	CGCGACGACAACGATTTCTC

 

Amplicon 5'

Amplicon 3'

TCTCCTCGCAATGGGAGAGAATCTTGGAGTATGTCTCGTGTACATTGTCCTCGTGAAAAGGTGTTGTCTCGCATATTAACTCATATCCTATAATGCCCATGGACCAATAATCGCAGCTGACCTACAAAATGAGTTCTTGTTTTAGCTATGAACTTTCCAAACCAAACTCTGCGATCGTACAATTCTACTCACATCGTGCATCGACTTGGACAGCTTGTAGGTGGATATGGTTTGCAGCAGCTCTGGAGCTATGTAATCCGGTGTTCCCACCGGCGACAAACTCAGCACATGGCCATCGCGATCCAAGGCAGCGGCATTACCGAAATCAGCCAGTTTAATATGGCCAAAACGATCAATCAGTATATTTTCAGGCTTTATATCCCGATGCACATAGCCCATCTCGTGCAATGTGTGCAGGGCCACTGTGAGCTCGGCCAGATAGAAACGAGCCAAGTCCTCATCGAAAGGGCCGTGGCGTGACATCAAGCTGAGAAGGTCTCCGCCGGGCATGTACTCCATGACCAGGTACAGATTGTCGTTATCCTAAGTGATAAGTTAATTCAAGTACTGAAATATGCGAAAATGTGGAAATAACAAACCTGAAAGGCATACTGCAGATTGATTAGCCATTCAGAGTTGCGAATGGACATGATATCGCGCTCCTCCTTGACTTGTGACGTCGTCACCACCGACTTTTTGATCTTCTTCATGGCGTATATGTCGTTGGTTTGCCGCTCCACCACCAGGTGAACGTTGCCAAAGTATCCTTGGCCAATAAGCGTCTTGATGAGGAAGTCATCTGCGTTGACCCTGAGTTTGCGGGTCTCCTCTATGATGGGACGAACTGGAGTAGGAAATTAGATAGTAATATAATGGGTAACTAGGCATGAGCTCTATTTTCAAGGAGGGAAACCGGAACATACAGGTAAATCTATAAGAGATTCTACTCCCAATAATTTTGTGGGCTTTAATAATGGAATATGATTAGCAAAAGTAGATTCTCTGACCCGAGGAAGT

TGTGGCTTCTGAGTTCCATTTCTATAGAATGGTAGATACTCCATCCGAAAACCCTGCTGACCAATGCAGTTGACTCACATTTGTTGACAAACTCGGCGATGTTGCGATCGCGCTTCTTCAGCGTATCCTTGTCGCACTCGTTGTACAGCAGACAGAAGGCGTCCAGGAGTCCCTCGCGGCAGATGGCCTCGGCCACGGCGGCGCTAGTGGTGCTCACGGGTACAATGCTTCTCCTGGTGGAGGCGGGAATACCTGATCCGGAGGCGCTTCCAGCGGGCTTCGCACAGACGCCAGCTCCTTTGCCCAGAATCAGGTTGTTCAGACGCGCGGTGCGCACGCTAATCGGCTCCATCTTGGGTGGCATGATGGACTCACTTTTATTTGCAATTTTCTCACATAAATAATACAATTAGAAATCCGTAAAATGCCAGAGCATTTTTCTGCCGTTTCGCTTTGAAAAAAATGCGCCGAGGGAAGCCGAAGAGCCAGGGATGCGTGCTCACTTTCCAGAGTTGTATTTAGGGTTGCAAGTAGGGCTGCCCTGTGAAAGAGGATAAAATTTGAATTTTAATGCAAACAGAGAACGGATAAATAATGAAATAGTCTTATTTACTTTTGGCAACCTTTTGAAGCGTCCCCTTTTATATTTTGCACAGTTTTGCACATAAACAGTTATTATTATTATTTCGCTGCCGCCGCCGCTGCTATACAACCGCGCTTTCCAACACCGTCGAGGCTCTCGCTCGTATGCATCGTTCATTTTCGACGGGTAGGTTTTCGCAGACGGACGGAGCAAAGATTTGTGCATCGAAAATTGCAATTTTCACATTTGGAGCCGTGTGTGCAGCATCACAGACATTGGAAAAGCGCTTGTCGCACTTGTGCAAAAACACAGCTTGATAAAGCAAACGCGGCTAGAAGGCGATTGGCCGCAAATTCGCCCTGGAACTGGAACGCAATTGCGTGTGAAAAAAAGACGGAAAAAAAGTAAAAGAGCAGAGACTGAAACCGTCGAGAAATCGTTGTCGTCGCG

 

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