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CRIMIC CR01495

Gene yl

CG1372

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:14193214..14199958	12E3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTTATGGAGTCCATGCACACGGAGAA	ATTCGAACCGTCTCCGTTAGCCTTCA

Images

Construct Design Sequences

sgRNA sequence	TGGAGAGGGCATAGCTACTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAATTCGAACTCGCACTCGC	CATTTAAAGCCACCCCCTGC	TGGAGTCGGATTAACCAGATTT	GGCTTGGAGTGACAGAGGTC

 

Amplicon 5'

Amplicon 3'

GAATTCGAACTCGCACTCGCTCTTTTCCTTAATTATACAAATTTCTGAGTGCTCTTCTGGTTTCTAAGCAATTCAGCAATTCGGGTTTTGTCTCTGATTTTTTATGACATTTGGCGGTTGAGGCGGCCAAAACGCTTCGGTCTTGTTTATCTGTCGTTGCTCATAAATCTTGCGGGCATAACCGTTATTAATTGTTGCAATGGGTCATCCAAATGGGTAAATGTTTCTGTGTCGGTTTCTGTTTCGGATCCCGTCGTTTCCCACTGCAATAATTTGGTTTTTTTTTTTGTAAATTTCCAGACACCGCTGACATCATGAACGGGCAATTAATCACGCTGTATTTGGTGCAATCCTTTTGCGATATTTGATGGTCAAGTTTTTAAATTGATGTCGAACGGCGTTGCCACCCAGGTACGGTATTTGGCATGCACTGGAATAAATTGCTGTGAGCTTTTATGATGAAACTGTTTTCAAATTTGGCGCCTTAAAATATGTAGTTTTAAATACCAATTAAACTATTACCAAACGGTTTATATCGGTGTACAACCATCTGGCATCTGGTCACTCCGAAATAACGGTGCTTTTCCAAAGCCCTGCCCTGATCGACGTGTTCCGTTGGAACCGAACGGTTTTCCTCGTTCAATTCGCTTTCAGCTTTCCAAGCTGCAAGCCATACTTTCTGTTTCCCATTCCCGTTTTAATTTCAATTAACCAGTCCTGCGTGTTAATCATCGTTCAAATTGTGTGTGTTTCGTGTTAAATGTGCCAGGCCGAGCACCAGGTGCACCCCAGCGAGCAAAGGATCCGGGTAGAGAGCCCGAAGATGACCGCCAGCCGGCGAGGATTCAACCTAACCTCGCAGACACGCGCTCACCCATCCAGTGGTGGCTCAACGTCCAGTCGATACGGAAATTGCCAGCGAACACATCTGATCATCAATGGACGCCACGTGGCAATCTCGCTGCTCCTGCTCGTGGGACTTTGCGGTGGTACTGCTGCCGGCACGCCGGGATCAGCGGACACGCGGTGCGATGCGGGACAATTCCAGTGCAGAGACGGCGGCTGCATCTTACAGGCGAAGATGTGCGACGGACGCGGAGACTGCAAGGACAGCTCGGATGAATTGGACTGCGGTGAGCATGGGTGGAATCAATGCCGTCGTTCAGCAGGGGGTGGCTTTAAATG

TGGAGTCGGATTAACCAGATTTCTTTAGCTATAATTTTGATGGTTAGGGAATCCTATCTAGAGATGCCACATCCCCATATTCCAATGAATTTACTAGTGAGACTAATATATCCAATAATATATAAACGTGTTGTGACAATCTTATATGTAGTTGAATAACATATAATCTTCATTCCAATCCGGATATCCAGACTACCGACTGTGCCGACCGCCACATTGGTTCCCGTGTGCCCAGCCACACGGAGCCTGCCTGGCAGCGGAACTAATGTGCAACGGCATCGACAACTGTCCCGGCGGCGAGGATGAGCTCAACTGCCCGGTGCGGCCAGGATTCCGATTCGGTGACACCGCTCATCGCATGCGCAGCTGCAGCAAGTACGAGTTCATGTGCCAGCAGGATCGCACCTGCATTCCCATTGATTTCATGTGCGACGGCCGGCCGGATTGCACGGACAAGTCGGACGAGGTGGCTGGCTGCAAGCAGGCGGAGATCACCTGCCCCGGCGAGGGTCACCTGTGCGCCAACGGACGGTGCTTGCGCCGGAAGCAGTGGGTCTGCGATGGCGTTGACGATTGCGGCGATGGAAGCGACGAACGAGGATGCTGTAAGTTAAAATTCTTTGTTGGATACTCCAAGCCTGATGCTCTTGAAACATGCGACTCGGCATCATTTCAGTAAATCTATGTGAGCCACAAAAGGGGAAATTCCTATGCCGGAACCGAGAAACCTGTCTGACCCTGAGCGAAGTGTGCGATGGGCACAGCGACTGTTCCGACGGCAGCGATGAGACCGACCTCTGTCACTCCAAGCC

 

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