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CRIMIC CR01497

Gene Snx6

CG8282

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:8218923..8218939	28F3	FBti0210406	86420

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGGCAGATCAGAGAGTTGTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAAACAGCCAGCCTTCTGT	CCTGTCCCTCCCTCTTCACT	TGAGGTTGTGTACGGTAGCA	CGAGGAGTTCGTCTGGCTAC

 

Amplicon 5'

Amplicon 3'

ACAAACAGCCAGCCTTCTGTACAATATTTCTCGTTTATATTCTGCTTCAAATGCAAGTGTTTACAATATATGTAGTTGTAGTTTATGCTGTAGAGAGGAATGCTTGTTTGACAAATATAAAAGCGCTCTGTTCACAAAACAAATGAATATTCGTTAATATAATACGTGGGCTAAGATAATAATGTTGCACTCGATTTGGCCTGTGCAATAATAAACTGTTTGCTTAGCAATTAAACAATTGATTTACTTCGTCTCCCGGATGTGTGTAATCGATGAGGCTTGCGTTTCAATGGGGTGTGTAAACAATGTGCGGGTGACTCATCCTTCTATTCCTTGTCTACTTCCGGCTCCCCGTCTCAGGCAATCTCTTTGAGCGCTAGCAGTGATTGGCGCAGATATTCATACTGCGTCTTGGCATGCTTGATCTCCAGCTCTGAGAGCTCGACGAGGCTGTGTTTAGAAAAGCGATAATAAAATGCAGAGCATAGCAAAATGTTTAGCAGCTGCTGACTCACCTCTTCTTGAAAGCAGCTACCCGTCGGTTGCGGAAACCGATCAACTCCTCCTTTCCACATGCAGACATAGATTCGAATTTTTCACAGGCCTCCGCCTGGGCAGTCTCGGCCTAATTGGAGGATAAACAAAAACGATTATATACAGGGAACACAACAAGTGGGCCACAGCAAGGGCATGCTAAAGTGAATACAACAGATTGAGCAACGGTGGAGCGCATGCAGCATTTACGTGGGGTGGCGACACATCTACTAAACCGACGGTTAAAACGTTAAATGCCAAATACCTCTTGCACCTCCAACGGCTGCAGAAACAGAACCACAAACACGCACAAAAAGTGAAGAGGGAGGGACAGG

TGAGGTTGTGTACGGTAGCAATAAAATACAAGAACGCAAATAATTTGTTATTTGTAATTAGTGAAGCGTGAGAATTAATTGACCCGCTGGAAACTTTTTGTAGGGAAAACAATTGCTCTTTAAACGATCTGAAACATAGTTCTGGAAGAACTAGTTGTGATCATCCCTCAATCTTACCGCATGAACATCCTTGTTTTTGGAGCGAGCCTTCTCCAGGTTTCGATTGGCCGCTTCATAAGCAGCCAAACACCGCAGTCGCCGGATAAGTAGAGCTTTGGCAGCGTCGCTATCCCGCTGATAGTAGCGCAGAGTGTCGCCCAGCTTGAGATCTTGATCGCTAGCCACTCGGGTTTCCAGATTCTTTAAAATATCAAGAGGAAAATTTATAAAATATAATTATAGCGAAAACGCAGCGTAGCGCACCTTGATCCTTTCAAATATCTCCGCTGTCTTGGCCACAAACGTCTCCACATTTCCCTTTTCCGTAGTCGAGAGCTGCGTCAACGCATTCGAAATCTTCTGATGCGAGTCGCCTACATCCTTGTGGCGTTGCGTCATTTTCTCCGTCCTGAGTGCTGCCTCGCGCAAGTGGCCGTGGTACTCGGTAAGGAACTGCAGCTCGTTCTCAAAGAAGTCATTTACATCCCGAACCGTGGCGCTCAACAAGATCTCGTCTGTGGTCTTGCCCAGGGACTTGACAAAGCCGCCAAAGATGGCCATCTTCTTGCGTGGCTTAGCGCACAGATCTTGGTCGTATTCCAGGAAAACCTTCAAGTGCTGATCGACGCGAAAGACGGGATGACTAGCAAGGCGGCGCAGGAAAACCTCGTGCATGGCCACCGTCTTCTTGAAGGTAGCTAGGTACTCACTGGCAAGTAAAGATGTACATTAGTATATAAGAAAATGACTATTTGGATTAATTGACACTTACGCCTCCAGCTCTGACTTCATCTTTTTGAACTCCTCTTTGGTCATGTTGCCTTCGCCCTCGCCAAGACGCTGCAGCTTCTCTCGGGAGGCATCGAAGTCCGGACGAGGTGGACAGGGCGGAATCTAAGAAAGATATTATAGCAAGCAGTTTGGAAATGTTTTTACCAAACACTCACAATGTAGCCAGCATAGTCGTCGTTCTCCTCAATACGGTCATGTAGCCAGACGAACTCCTCG

 

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