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CRIMIC CR01498

Gene lace

CG4162

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:15499127..15505018	35D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CACTGCAGTGTTTTCCTCGCGTGTTT	ATGGGTCGGTTCCAGCAATCCTTGAT

Images

Construct Design Sequences

sgRNA sequence	ATAATGACTTCCATATGTTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAGTACGACCCCAAAGCAA	TTGGGGATCCATGGAGTCCA	ATCTGGATCCCACACCATGC	GGCTTGGACTCCTGATCCAG

 

Amplicon 5'

Amplicon 3'

GGAGTACGACCCCAAAGCAAACACACACATAAGTACGTAGTTTGGGTGCACAATGTGTGTGGGGAGAGCGGCACAATGCTGGTCTGGTCATAATATTGTCATGCCACGCCATGCCCGGCTCGCCTTATCAGGCCCATTCCACCAGTCTTTTACGTAAAGCCTCGCAGAATGCGATTCGCTGGTGAATCACAATGCTCCGCAATCTCCGAAGGCAGCGGTTTTGGGTGGGCGTTGATTTGTCGGTGATAAGAGTTTTGTTTTAAAATTCATGGACTCTCCCTGCGTCTGGGCTGCCAAAGAATAACTGTTGGCGGGTATACTTAACCGATTATCATATTTTTTATTATTCATAACTTTTTAAAAAGATAAGATAAACATATTATAACATTACATTCATTGGGTAATTCCGATACAATTCGATCTTTGGGGAGTGAGCAATTGTTAGTTTTCTTTTAAGTACCTAAAGATTTCATTATTGGTAGCTTTGTATTTCTTTTTTTCGGAAAGTAATACAATGGTTTACATAAAATTTATTTAATGAAATAGTTCCGTACCATGGCGACAACTTATTGAAATCATTCAGCTTGATTTAATGGCAACTAAAGACCTTTTCAAATTTTTAATCACACGAAAATAATGTTAGCGCGTGCAAGTCTCATATCTCCAATATCAACGGATAGAAAACTTTTATCAGCTCGGCAAGATTACAGTATGCATGATTTCAAAGGTAAAGGCTTAAATAAAAAAGAAATACTTAAGAATATGAAATTGCCAAAGCAATTATCTACTGACTCACAGCTAATACTTCTTGGAATCCCCACAAAATCGGTTAGAACTTGGCGTGCGCTGGTCGATTGTCAGTCGTTATCTTGGACTCCATGGATCCCCAA

ATCTGGATCCCACACCATGCTCCCTACTTAGCTGTCCTTCTGTACGCGACCCATAGCGTAATTAGTATTACACTTTTTCTATTTATTTATTTATGTCGGTTTCGATTTTGATCTTTCTACGGTAGCTTCGAGCTCGTCTATATACCCTAACACTGGGACTCCATTAAATAACTAGGCATCGCGTAACCCACTTGACTTTTCGCTCCCCGCACTCCGTCTCATCGTATTTAGTAAACAAGCCCGGTGGCAAATGATAATAAACACGAAATATTATTGTCGCCGACTCGTTATTGTTATACGATTAAGCTGACGTTCAACTATATATATTTTTCGGAATATATATCTGCAGTTTGTGTTGCTCAGTTAATGAGATATATATATGTCTCGTATCGGATCCTTTGGCCAGCAAAATCAATAAGCAAATGATATATACACTTAAATAAGTACGTTAATTATAACGCTCTATTTGTTAGCTGGTGAGGTTGAAGTGTGCCAAGTTGGGCACACAAATACTATATACATTTATGGCTTCCCCTGTATGATTTTTGTGTCTGTCTCTTTTTGTGTGACTTCATGTCGAACTCGGCTCATGTGAGCGGTGGAGCAAATTGTTCAGCAGTTATCGTGTGATTGTATTTATATATGTACATACGTACCTTGCATATATGGATCTTTTAGTTAAATGTTAATTGACTGATGAATTGACTGAACGCATAGAAAGGGCAAGCAAGTGAGAATGTAGAATTTTTCATTTTTTTATAAAAGCGTTTACGCATGAGAACTATAAGTTTTGATATACATACTCGTATAAATGGGTATTACTGTGCTGATCCCTTTGAAGCGAACATGTTTACAATCTGTCTTACAATTTCCCATCTAGCCCGTCTAACAATATTGATTATTTGCTCTTTGATGAGTGATTTTCAATGGAAATTATGAAGATTCACAGTGATACCAATGATAGCGATAACCAGCCAATAATCGTTCCTCTCCCCTCTTACAGAGTCCCAGCTCGATCAACTACCATCCGGAAAAGAAGTCGATCCCCAATGGGCATGCCTCCATTCTGGATCAGGAGTCCAAGCC

 

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