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CRIMIC CR01500

Gene mdy

CG31991

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:16826964..16816660	36B1-36B2	FBti0212927	91255

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGAGAGAGACAATCGCTGGAAGAGC	GATCGCATCCACGAAAGTGACAGGAA

Images

Construct Design Sequences

sgRNA sequence	CAGCGTCTGCTGCAAATAACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTCCTCAAAGAACTCCGGC	CTGAGCGCCGATCCCAAT	CGGCCAGCAGCTGTAAATTT	CTCATAGCAGAGAGTGGGCG

 

Amplicon 5'

Amplicon 3'

TGTCCTCAAAGAACTCCGGCAAATCTTGGGAGTTGAGCGAGAAAAACACCTTGTTCACAAGGACCAATGAACCGTAGATGACCTTCAGGGCGCCGGCATTGTTCTCGTGCACCTTAGTCAACTGCATTGTGGCCTGGAGCAGGTCTGTCAGAGGTTTAGCCATACGATCCAGTACAAATTTGATCTCCTCCCACAAAGCCTGCGACTTGAACTCATAACGATAGCGCTTGAAAAGCGAGTGTGCGGTTTGCAAAACGCCGTTGATGACATTGAAATCACCAGAGGCAAATCTCTCCACCATCTCGTCGATAAGCTGCGGCCATTTTTTGGGAAAATCATATTTGCCTATGATGCTGACCGCGTCGCTTAGTTGTTTTTGCAATGCCACCGGTGAGTGGAGCATTAGCGTAACGATCAAGGTCTTAATTGTATTCCTGTCACTTTCGTGGATGCGATCCGGTCCGTCGCTGTCAAGGTGGGCTGCCCAATTCCGCTTAATATAGTTCTTAAAGGCAATGGCTCCCGCCACCCGCGTAGTCATGTCCATTTGGGCTTTGTCTATCAGATTGAGCAGTAGGATCGGGTAATTCTGCTGTAGTTCCGTGGACTCCAAAAGTTTTTCGGCTATAAAAGTAGAGAAGGAAATGGTGTGTTTGTTAGAACTATTGTACTTATGTAAATCGGATGTTGCAAATACAAATATATAAGGAACCGATAGGAGCTTACAGTGTTTCTCGTTAGACTAAACATATTTTCAATTCAAGCAATAAGTTTTTTATACAATATTTTCAAAAGCTTGGTTATGGTGGTGCGGAGTCTATAGGCTTGGTATGCCTTAAATAATCCTAAAACTAGGTCTATGGTTTTGAAAGGTGGCTTGGCAACAGTCATATCAAGATCAGCTAATCTCGATTCAATTAATAGAAAGCGTGACTGTATAAATTCTTTGACATAGGGAAAACTTGTAGACAACTTCAAATGGATTTAAAAACCGTATTTTTCTTTGGCGTCGCCAAAAACCATTTCCCGCGCACATACACATCAGCACCCCTGCACGCATGTGCAGTTGTGTGTGTGCACATGCATTCCTACACACACACACTCACCCGGCCGGCGGACATTGGGATCGGCGCTCAG

CGGCCAGCAGCTGTAAATTTGCCTCTGTCACTTCCATTTTGAGTATATTTTCTTTTCGATGGTCCTATTTGCTTTAATTTATATCACTCGTGGCTTGTTCCTCCACACTCTTGCAACATGAAATGAAATCGCAATTCGTTTTCGCACACAATGCTCGCCGATCTGGGGATGGAACTCAGGGCGAGTTTTCGATATCGATTGTTTATAGTTACTGTTACTAGGATTCGATGGTTTTAGATTTTAAATGTTCATATCAGAAATATCGCATATTGAACTTTACTAATTTAGTATCATTTGATATGATATCAATTCCTGTTTTTTTTTTTCAAAAACCAACTGAATGAATTAAAAATTAAAACAAATTTGTAATTCTTTTCCAGGAAATAATTGCAGAGGGCTTGGGCTTGTTCATCCAGATAGTGAACATTGTTGTCTTGGTTTGCCTACCGGTGGTAACAATTCACCTAAAAGGACATGCTTTTAGTTTGAGTGAGAAATTAACTTAGTTTAGGTGTATTTTAAAATATTTAATTATCTGTTAATCTTTAGTGGGCGCTTCAACAGTTTGCTTCTTTTACTCTGTGTTGTTCCTAAAACTATGGTCCTATGTGCAGACGAATATGTGGTGCCGTCAGACTTATTATCAAAAGAATCCGCGGGAGCGTCGACCAAGCATAACTTTGGCGGAACTAAGTATGTTGAGATCTCTCAACGGAATGTCATATATTAAAAATTCATCTTTCATCCAGAAAAAGGAGTTTTGAATGGAGGTGAAGAAGACGAGGACGTTTCCAAGCTGGTGCAATATCCTGATAATCTCACATACAAGGATCTCCTGTACTTCCTTTGCGCGCCCACTCTCTGCTATGAG

 

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