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CRIMIC CR01501

Gene lilli

CG8817

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2947481..2947494	23C3	FBti0210407	86421

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CATGGTGCAGCGAGAATAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCTACTGTCTCTGCCAACC	CCATGCTGCCATCGTTTACA	AGTTGGCCAAACCTCTGGAG	CCCACATTACCTCCGCTACC

 

Amplicon 5'

Amplicon 3'

TGCTACTGTCTCTGCCAACCGAAATAATGGCTTAAACCAGTACAAAGAATAAAAGACCAACATTAAAGACCGTAAGTCAAAGTTCAAGCATTTTACTATATACTTTTCGTCCTTTTTGCAGCATGGAGGAGTACGAACGGCGAAAAAGACGTGAGCGTGAGAAAATCGAGCGGCAACAGGGCATACAGATTGACGATCGGGAGACTAGTCTATTCGGGGAGCCGCGTCGGCTGACTGAGGGAGATGCGGAGATCACCGCCGCCCTGGGTGAGTTCTTCGAGGCGCGGGAGTACATCAACAATCAGACTGTGGGAATCAGCCGGAGTGCGCCCGGCGCCGGCAATCCGCGCCTGCAGCCCAATCTGGCGCCGCAAGCCAAATCCCTGGGGCATTCACCCTCCTCCGCCTCCTCAGCAGCTGGGCCCACTGCCGCGTCCGCGACCACTTCGCTGCCCGGCCAGCAACAGCACTACCAGCAACAGCAGCGACCGCCTACGTATGTGAAGCAGGCGGACAATAAGCCGCCGTACAATGGTCGCGGCGGCTATCCTGGGCAGCCCATGAAGAACGACATTCCGTCGAGTAGTGGCATGGCCCCGCCCCGCGGCCCGCCCAGAACCAGTTCCAGCAACAGCAACTCGTCCAGCGTCACAAACAATGCCAGCAGCGGCGGAGTCCCGGCCAGCACACCGCTGGGACCACCCCTGTCCACCCAGATGCCGAATGGGCGAGAGAAGTCCTTTCTTGGTCCGCCGGCTCCGGCGCTGCACAACGGCACCGGCGGACGCTTTGTGCCGCCAGCGGCCAGTAAGCGACCTGGAGTGGGCCAACAGCCGCCACCACCGGAGGTAATCATTACTTAACGTCTAACACTTGTAAACGATGGCAGCATGG

AGTTGGCCAAACCTCTGGAGGTTGTGCTTACCAATCGCTGCAACGTTTACAAATGTCAAACACCTTTGGATTTTTTTTTTCTAAACCACGATTTCTTTTTTTTTTAACCTTGCAGAAGGATGTCAACAAAATTATCAGCGACATAGCAAATATCTTCACGGTGCAGCCACTCACATTGATAGCGGCGACGCCACATGCGCCAACGCGCGAGAACTACAACCTGCTGGCGCCCAATAGACAAAAGTATGCGATGGACATACCCAGCTCGCCACCCTCTGCGGAACCCTCTTCGCTGATGACGCCACTTTTTGCGCCCATTACCTCGCCCATCGCCCCGTTGGTGACAACACCGCCACAGGCAAGCCAAATGCCTCTGGGCGGAGCAACAAGTGGGACGATACTAGCGGGAGAGGCTCTGGCACCCCTGCACCAACTGCCACCCACTATGCCCAAAGCAGCTTCCGGAGTCACGTCGCCGGGACCGGTCAAGCCGCTCAAAACGGAAAAGAATCACTCGTTGGAGAAGCAAGACTCGTGGTATGTATACCTACACACTTGGCCATTCAAATTGTTCATACTTTGTTCACATTTTTAGCCTGGAGAACGACCTGGAGCTGTCCGAGTCGGAGGATGAACAGCGCAAGAAGGAGGGGTAAGTCGTGTGGCTGTGCTCATTGAAAATGAATAGGCTAAATAATGTTTTCCCTATAGGCGATCGGGTGGAAACAGCAGCAATAGTTCCGAGTCCGATTCCAGTGAGTCGGGCAGCGAGTCGAGCAGCAAGAACGATCTGCAGCATCATCCAAACCACCAACAGCACCATCACCAACTGCAGCAGCAGCAACAGCAGCAGCAGGCGACCATGCAGCAGCAGCAAGTCCTCCAGCAACAGCATCGGTCCCAACCACTGACGTCCAACGGGGCCCAGAACAAGAAGTTTAGGCATGAGATCATTGCCCGTGGCAGTAATACCATTACGGGACTCCTCAGCTCCAGTGGATTCGGTAGCGGAGGTAATGTGGG

 

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