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CRIMIC CR01505

Gene Rab9

CG9994

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:19434055..19434073	37E1	FBti0210409	86423

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TTGCTGGTTCACAGTTCGCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTCCAAGCTGCTGAAGGTC	ACTATGCGAGATTCCCGTCG	GCTGGGCACAGGAAGAACTT	GCCTTCGATGACGTCTCGAT

 

Amplicon 5'

Amplicon 3'

AGTCCAAGCTGCTGAAGGTCGTCATACTGGGCGACGGAGGCGTTGGCAAGTCCGCCCTGCTCACCCGATTCGTCGCCAATCGCTACGAGGAGAACAACTTCCACACCATCGGCGTGGAGTTCATGAACAAGGACATAGTCGTCGACGGGGAGCGCTACACTCTGCAGGTGAAGACTACGACTATGACTATTATGCAATCCAACTACTGACTAGGAGTAATAGTAATACCGATATTGCGGCAAGGCCGTAGAACTTTTGCAATTCTTATGGTGAATGCAACAGTTGGCCAAGCGTATTTCCTTCAAGTGTATTTCACTTAAACACTAACCCCCTCCCCCCCCCCCCAAACGGATCTATCCCACCGTCGCATGGTTGTGCCCGATCCACCGGCATTTTGTTGATGCAAACACTTAGAAACTACACAACGATTAAGCCTTGTTTTCCATTCCCTTACATTCGCGGCACAGTGGAACTTTAGTAAGTTAAACAGGGTAAAAACAGAAGTGCTAACAAATTAAACCATTTAAACAAGGAAACATTGACTATACATTCCTTGAACCATTTGAAACACTCGTATACCAGAGAAAAGTATTATCTTACTGACACGTTTTTGAGTCTTGCATTTGAAATTAAGAAAAAAATATGTGCAATCAATTAATTACGTAGTTTTTTCCACTGTAGCTGTACCTACATTATGGGGGTATCTGTTCCATCTGCCCCATCCCCACTCACACACACACATATATGAACATTAGATTTCTACTCAATTAGCGTTGAGAACGGAATGGCATTGCAGAAACAAAAAACTGAAATAATAACAATAATGACAGTTTCGCCATTCGGCAAACAAGTGAGAGACTTTGTTTCATCCGCCGACGCGGTTTCCCCCGCTCCATCTCATCCGCATCGCAGTTGTTTTCCATTTTCCATTCGGACTGTTGTCTTCAAAAGTATCGAACTAAATGATTTTCACAGTTCGCCAAGCTCGGAAAACTCATGAATATGCAGCGCGTCTCAGTGAGTAAGCAAAATGCAGCCAAGGTGTCGCAAATCGGAATAATAATTCTAATTGGACCAGCAGATACATTATAAAGTAGGAAGTAGCGAATGTGGACTGATTGATATACATATGACTAATCCGACGGGAATCTCGCATAGT

GCTGGGCACAGGAAGAACTTGGCCAAAAGCGGATTTCCCAGTGCTGCAAAAAATCGTGGAATCACTGGGGTGTTTTTTGCACTACCTATATACATATGTACATATGTATGGAGTCACTTAGTTACCATGGGCAGTACTCACACCCTAAAAGGGTTATTCCACCCATGTGTTTGGAGGCCAAAGGTGTTTATATTAGATAAAGAAGTTTAAAGTATCCATTGTTAGAGCTAAAAGTATAACCTCATGTTCGAAAGTATAAAATAAATAAAAGTATAAAATAAATATTTAAAGTATGTATGCTACTCGAAAATTCAGATTCAACGAACTATTTCCTGAGCTAATTGCTTATCACCACCTCGTTATCAGTTCAAAACTAAATATTTAATTAACACACAAGAAATGACAGTAAATGTGTTGATATAAGTGAGTCAGTCAGGCATGCAAACACTCCATAGGTTTTCAAACGGCGAAAGTGTTTGTTTCTCAGTGAGTTTGCTTCTAATCGAATGCAAATTTCAATTGAGCGCCATTTGAATCGCCAATTCAATCAAACTTAATTGATGCATTGCCTTAATTGGAATTTCCCCAAACTAGATATGGGACACCGCTGGCCAGGAGCGATTCCGGGCTCTACGCACCCCGTTCTACAGAGGATCGGACATCTGTCTGCTCTGCTACGCACTCGACGACCGGGACAGCTTGAAGGGATTGGGTGTGTGGCGCAATGAGTTCCTCAACTACGCAGATGTGGACCAGGACAAGTTTCCATTCATCGTGGTGGGAAATAAGGTGAGCCATGCTTGCAGAACGTTCATCTTGAGTTTAAAAGGTTAATTGCTTCCAATTGTAGAACGACATTCCGGCGCAGAAGCGACAGGTGAGCTCCGACGCAGTGCAGCAGTGGTGCGCGGAGCAGAAGGTGGCCTGCCACATCGAGACGTCATCGAAGGC

 

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