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CRIMIC CR01506

Gene Hil

CG30147

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:20612829..20612850	57A7	FBti0210410	86424

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACTAGCCTACATAACCACGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCATCAGACACCTGTTCGA	TGGTTATGTAGGCTAGTGTGCT	AAGCCCAGGAACATTGCACA	ATTGCCACCTGCTGTGTGTA

 

Amplicon 5'

Amplicon 3'

GCCATCAGACACCTGTTCGAAATTTGTTAAATTGAGCGTTGCAATAAAAATAAAAATATTTGCTGTTGTCTCTGCCCGCGGTGGTGCTACTTGGCGGATTGGTGAGCTGGTGAGCCGGTGGAGCTGGGATGTGGTGCTGGTGTGCTGGTCAGTCGCGTGTGGCATTCTCGAAAGTGAAAGAAAAATCCGTTTTCGGCATTTGCAATGGCAAAGAAAAGCAAAACGTGTGCGATTTGAATTTCGTTTTTACGTAAAATGCGATTATAATGCTTTCACCCGAGCATCAGGCGGATAAGAGATTACCATTCCAGTCGAGGCAAGTGCCAAGTCATGGATAAAGATGCGAGCACCGATAAACGCCAGCAGCTTTTGGCCAACCAGTCGGGCTTATAATTTATAATCTGGCCGAGAGATCATCCCATCACATGGCGCGCCACCACTAACTAGATCAATTTGGGTATCACATTTATCCGTCTTCAATTTTCCACTTTGCAAGCCAATTTCAAATTTCTGAACCGTTCAAAAAAAAAAAAAAAAAAAATATACCAAAAGACTAAAGTACTACAGGTTTTTTAGCAGATGTTCAATAATTCAATGTTCAATAATTTATATGGCAAAGTAAAGCACGGATATCTTTTTATATTGCCTAGACAGATCTGATTCCTTATCGCATCTTAGTAAAGTTATAAGTAAACTATTCATATTTATCTTTGTTAAAGTCGGGAATTTTGTGTAATAGTAGTACCGACAAAACTGTATCTTCAGACTTGGCCACTTGAGCAATCGCACAGCAAGCATTACACACAGCCAAGTGTAAATAGCACACTAGCCTACATAACCA

AAGCCCAGGAACATTGCACAACCGCTGAAGCTTGGAGAGAATGGGAGATACACACTTGACCAGCTGGCCCCGCTAGCCACTTAAAATTTCATTAGATGTTCTGCACTAAATGTGCTCTTAATTTGTGGCCACTGTGGCTCCGCATCTCGGGAGATGGATGGATCACTTCTTATGATTATTGTACAATAATTTAAGAACAGAAGGATATTGCTTACCAGGTAAAAATCAATCGGCTTCTCCCGCGCCTTGTTGTAAGCCTTTTGTATCTCCGTTTGTTTGAGGGCGTGCGCGATGTGGAGGGCACTGGCGTCGAATCTTCCGTACTTGTAGTCAGAGTCATTCTGCGATGGAGAACTGATCTCCCGGCTACCGTAGCCATTCGGCGTGGACTGCCGCGAGCCACCCAGTGGGCCACCTGCACAGATCCGGTAAAACAGTGTTGTTAGCCAAGTTGAGAGCACATGACAGCCCCATACTCACCATCCACTTCGCTCCGCGTGCCTCGGATCTGCTCGTTCACGAACTTGTTGGTGCGCGGCGCATTCAGGGCCTGGCGAATACCCACAGAGGTCTGGTCGAGATGGCCGGGCCCGCTCTTTGGTACATGTGAACTGCAATAGACCTAGAGCGAAGGTGTTTCCGAAGATTATTAGTTGTTGCACATATCGCTGGGTCATATGATATAGTGCATACTTATTCAAAAAGATAAACTTCCAACTAACTAAACTGCATTCATTAAAAGATTTAATCCTCATTACTCATTTCATTATACATCTAGAAAAAAAGGAATGCTCTTTCTGAATCATGTCATCTAATTGCTATTTTGAAGCCATGAGATTTCTTAATGGGCACCGATAATTATCTTCAATTAAAATTTCCTTAAACAAAAAGGAATTTTCAACTAAAACACTTTTGGAATGCATATATCTAGCGTCCAAATCGAAACACACCATTAGCCTTATTGGGTTTCGCTTTGAGTGCGTGAGCAGACCCCAAAAGTCAGTCGCTTGCAAATGCAATTTTAACCACTTCACCTACACACAGCAGGTGGCAAT

 

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