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CRIMIC CR01508

Gene tsr

CG4254

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24046805..24044453	60B5-60B5	FBti0212928	91256

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTCACGACGCATGTTGATACTTTGCC	TTCGGACATCAACCTGTGCACCCTTA

Images

Construct Design Sequences

sgRNA sequence	ATGTACATCAGTGAGGTACAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCACAGTGCTCGTACAGTT	CCTCGTGTCTTATGTGGGCA	TGCGTATGTAACTGACTGGC	GCCGGTCATTGTGCACTTTT

 

Amplicon 5'

Amplicon 3'

TGCACAGTGCTCGTACAGTTTATTGGCGGTCGGTGGCCCGGAGTTTCTCCTCGACGGCTTCCCGGGAGGCTTCGGAAAGATCAGTGGCTTGTATATACTTTTGGACGCCCACGAGCGACTTCTTGAGAGCATCGAAGGAGCTGGAGTACAACATCTTCTTCTTGACCTGTGGGTGTGTGATATCGAAATGAGTTCTGCGCCATTACTTGGCAACTGGCTATTCACATACCTTGGCAGTATCGGGACACCACGACATAAGGAACAGCTTTTGCTTCTTTGAACTCTCAGAGGTGCCTTGACACTGGTGCATGTACTCGAAATCGAACAGTCCGTACCTGTGCAAGCGAATAGATGTTAAATTAGGTTAGTTTCGATGGGTCTGGGTGTCTCTAACCGGCGGAACTTACCGGCACTCTCCAGGTCCGCATTTCTGGATATCTTCTAGAAACTGGTCGTACTCGGCGTTGCGATCTGCCACAGTCTCCACATCAATCTGCTTTTCATCGCGAATGTAAAAGATGACATAGCGATGCTTTTTGTCCTTCTTTATTTCCTCGTATGTAGTCTTGCAGACATCAGACACAGTTACACCAGAAGCCTTTAGAGAGAGAGAAGGTAGAGATGCAAATCAGAGAATGTATTTTGAATGTTCAATGCGCGCCAAACTGTCAACCAAGTGTGTGTGCATGTATGTGATACAGGGTGCTCTCTTTGCGTTTCCCCAAGTATTCCCTTAAAACAAATGTTCATGAAGTACCAAGTAGTTGAATTGTAAAAAAAAATAAGTGAAGTTCAATGTTTTATGCCCACATAAGACACGAGG

TGCGTATGTAACTGACTGGCAGACATAACTGCCCACCTTCAGCTGACAGTATATCCGTCCCTTTTCGACTTTGCAATTTTATTTCTTTGATTGCCGGCAATAACAAACATAAAGGTAAATAATTTATGCTACTTTATTTGCATTGCCACCGCAGAACTTTTACCGGTTAAGGCGTGCGCACACTTGGCACGCCGATTTATCTGGGAGTGGTGGGCGTGGCACCATTTACAGTTAGATTTTCCTTTCTTTTTTTCCCGGCGCTCGGAAAGTTCAAGGGTAAGTTCACAAGTGCCGGCAAGTTTAAGGGTGAGAACAAGCCGACAAGAGGGAGAAAGAAAACTAAAAGGGAGGTGGCTGTTTCCCCACGATTTCGTTGCGCTCACAAAACGGTGGAAAATGCCCGATTTTCCATAATATTCGGAAAATGTCGAGAAAATCAAAATCGTCCCACATGCAAACAGTCGTCTGCTGCACACACACAGCTAAACATCATGTGTATGTATGTATGAACATCCGCAAGGCGGCAAGGAAACGATCACACCTTAAATATGTATGCCTGCAGATACATATATGGATATATATTGATACATATTTCTGTATGGTAATATGGACGTACATATGTACACACATTTTGCGTCTGCGAGAAAATAAGCATTAAGCATTGCCCCGCAGATACATACATACGCACATATTTATAGCCATCGAAATATAAAAAAAGCGGTATACCCACACACGCTCAAGTGTGTAAAAATATATATATGTATAGACATGAAAATCCAATGTGTGTGCATTTCTCTTTCCAACATTTCAATTTTTAATCGCTCCATTTTTCCAGCTGCCACTTTGGAGCATTCTTTCGCGATTGCCTTTCGCGAATTTGCCAGTTATGAGTGTGAGTGGCGGCTGAGAAGTATGAAACACTCCAATAAATGAGTAATATCCGCTGCTACCAATGACTAATTTGGTATATATGTATACGCACACATTCATGACCATGTATATGCCCGAATATGGCCAACGAACATATAGGCAAAAAAAAGTGGAAATATATGTATGATTGATATATACACACTTATCTAAATATGTATGTATATACAGATTTCAAAGGAGAGATGCTTATATGATTCATCGAATCGAAGACATTATTTGCACTCCACCTCGCTTCCAAAAGTGCACAATGACCGGC

 

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