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CRIMIC CR01510

Gene AANAT1

CG3318

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24156307..24156322	60B13	FBti0210412	86426

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CACGACAGCCGAATACTGGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAAGGCAGCCACTTCTGTA	CCACAAGATTCCACATGCCC	TCGGCTGTCGTGAGATGTCT	GCTTGAAGACCACCTCTCCC

 

Amplicon 5'

Amplicon 3'

CCAAGGCAGCCACTTCTGTAATTTGGCATGTGCGAATTTTCCAAATTCAAATTCCTTCTGGATCGGTTTAATGGCCCCTCCTCAGCGAGTGCGATCGGCATTTATGGGGCATCAGTCAATGGAACGTGATTTTATGAGGTGGCAACTATGAGGAACCTCAAATCCAATAAATCACTTGAATCTGTTTTGAAGCAGGCCTAAATATAAGTGTGCCTCGAAATATTCCCAAGACAGCTGTATAAACAAATTGGCTATCTTAAGTATACTCCCTAGATGTCCCACCAGTTTTAACTAATAATAGAACTAATAATTAAAGTAAACTACATCTGCTAGAACTGCGACCTTAGTTTCGGTTCTATAATTTATGGCTCCTGTCGCTGGCGAACATGAAAACAAAATTTGAATAACATGGTCGGTGGAAAATGGATGTGGAAATGGAAATGCACGGGTTCGCCATCCATGAGCTGCGAAATATACAAAACAAATTAAGCTGCCTTTCATCTAAGAGCCATAAATAATAATGTTTCCTATGCGCCGCCCGACTTTCCTCCGACCGCCCATCCATACATCTAAGCATCCAACCATCCCTCCATCCATCGAAAGTATCTGAACGATTTGTTTTTCTGGCGCTTTGCAGCTGAACGACTTGTATCCGATCGCCCGGCTGACACAGAAAATGGAGGACGCATTGACCGTCTCTGGGAAGCCAGCCGCATGCCCCGTCGACCAGGACTGCCCCTACACCATCGAACTGATCCAGCCGGAGGATGGGGAGGCGGTGATAGCCATGCTCAAGACCTTTTTCTTCAAGGTAAGTGCGCTCCCGCCCATCGTACATTGTATTATAATCAGGGGGCTGCTTGGGAGATTGGTGTGCATATCGTAGGAGCCAATCAAGCCGAACACCTGGCTCCGGAATTTATAACCGCTTGCCGAATAATTTGAATCGAAAGGCGGCGCACAAAGGTGAATTTGAATAGTTTCCAGCGAATTTCAATTAGCTGCGCGTTCGTCTTAATTAGGCTTTAATTCAGATCGAAATTCGCACACTAAACTTGAATTAATGCGTGTTGGCCAATCCCTGGGAATTCCAGCCTCCGCCGGGAGCTTGCCCCGTTTTGGGGCATGTGGAATCTTGTGG

TCGGCTGTCGTGAGATGTCTTGACATGATCCATGTCACGGCTAGCTTTCAGCTGGTCTTGCATCACGACTCTTGGCGATCTGAATCTCAATCTCAATTGGAATCTGAATCCAATGCGCTCGCTTGATCTTGACTGGATCAGGTTCCAGTTCTCGATGGGGCCGGCCAATGAGAATGCTCCCTGCACAGACCCAGACCAATTGGGCACTCAAGACCTATATTTATACATATGTACATTGCATATACATATGTACATATGTATATACTGCATATTGATTAGTATGCATGCCACGCCCGCTGGCCACAGCCGCCCCCCTCCGGCGTTGAGATGTTGATATGCAAATCGAGTTCCGTTCGCCATTCCCCAGCTGCCTCCATAAATTATTGGAAAATCTTTAGCCGATGCAATGCGTGCATAAATCACTTGAATTGCCGATCAATTGCACGATCGTGTCGCCTAATAGCGCCGCCAATTTGTTCGGTCAGCAGATCCCCAGATTGACGAGCAATCGGATCCAAATGGGTGGGCCAGTGCACTGATGTCTGAAATCTAATCCGTTTTCCTATTCCGCACAGGATGAACCGCTGAACACCTTCCTCGACCTTGGCGAGTGCAAGGAGCTGGAGAAGTACTCCCTGAAACCGCTACCCGACAACTGCTCCTACAAGGCGGTCAACAAGAAGGGCGAGATTATCGGTGTGTTCCTAAATGGACTTATGAGGCGTCCGGTAAGTGAACCCATTAAGTATGATATGAATGATGCATGACTTATTGTGCTCCATCTATGCATAGTCCCCCGATGATGTGCCCGAAAAGGCGGCCGACTCCTGTGAACATCCCAAATTCAAGAAGATCCTCTCGCTGATGGACCACGTGGAGGAGCAGTTCAACATCTTCGACGTGTATCCCGACGAGGAGCTCATCCTGGACGGCAAGATCCTGTCGGTGGACACCAACTACCGGGGCCTGGGCATCGCAGGCCGCCTGACGGAGAGGGCGTACGAGTACATGCGGGAGAACGGCATCAATGTGTACCACGTGCTCTGCTCCAGCCACTACTCCGCCCGGGTGATGGAGAAGCTGGGCTTCCACGAGGTGTTCCGCATGCAGTTCGCCGACTACAAGCCTCAGGGAGAGGTGGTCTTCAAGC

 

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