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CRIMIC CR01512

Gene Corin

CG2105

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:7556397..7556405	43D5	FBti0210414	86428

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CAGGTGCAGAGGATGAAGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCAGGATCTTCTTTGGCCT	GCAGGGGACTAAGCCCTG	CATCCTCTGCACCTGGATGG	CTGTACGCATCCCTGGGATC

 

Amplicon 5'

Amplicon 3'

GCCAGGATCTTCTTTGGCCTATAGTAAACGACTAGATACATCTACTAACTATGTGTCAGATTAGAGAACAAATAATTTAAACTTCTAGTTATGTTATGTATCCAGAAGTTTGTTTATAAAGATCTAAAATCGCAACTGATAATGTAATCGCAATCTGTATTTACCGCGAATTTACGAAGAAGTGGTCAAATTTCTACCGGGCTCAGAATACTTTAAGAAAGCCAGATTTTTCCGGAAAAAAGGATATATTTTGAGCTTTTCCACTGAAAAGAATTGGCGAAGCAATCAGCTTGGCATTACTTTCTCAGTTACCAAATTAAAGAGCAGGTGGTAGTAAGAAAAAAGGATAATACGATGTCTGTTAATTAATATAATATACGAGTTTCCTTTACACATTTCAAGCGAACTGCACTTTTTGAGAATTTTATACGCAGGGAAAAGAAGATAGAAACTTTCCGCGAAACATTCGAGTTTTTCCCTCACAATAGCCATTCATTTTGAAAAGTTTTTTTTGGAAGTGCAGCACAATACAATTTGCTGCAGAAATGAAGACTGCGAAAAGACAAAGGAAAATGGAATTAACGGATGTACATAGAACTGACCCTTAATGAGAAATTTCCTCTTCTGCATCTACGAAAATAAATAATCCTTCTATTGCGCTTTTCACGGCGAAGTAAACAAAGCTGAAATTTCGAAAAGTTTCCTCAGTTTCGCAACCGCCAAGGAAACAGTATTCAAATTTAATACCCTACTCATCCTCGTTTGGGTTATTCGACTGGCGTTGCACCGTCAAGGGTTTCGAGTGAAGGGCAAAGAAGATGAGGGATTAGCTATAGTTTGGCTTTGCATGACTCAAGAGATCGATGTGGACGGCAGCCAAAACCACTTGCCTCTGTTCCTGATTCATTTTCCAGCAGGAGGACCTGGCCGCAAATCCCCCCAAAGTTATTTCCTGCGCGTCAGAATTTCTGCGCCCGGCTCAGCCAGGGCTTAGTCCCCTGC

CATCCTCTGCACCTGGATGGGGCTTAAAATGCAGAATATTGCTGCTGGATAATATTGCCACAGTGGTGGATCGAAGTAGCAAACATTACAGTGGAAACAGGTTTCTATCACCAGGGCAATATATCCTATATCTTTTTAACATTGTGAAAGGCACTCAACTGCATTACTAAGAAATTTCATTTATTATTTCCTTATTAGAGCATTTTAAAGTTACAGCGTAGGTTGTTATTTATATATTTAATATTTATACCTTATAACCCCGTTTAGTGATACAAAAGTTTCATAAAAGCTAGTCATATTTATGTTTCTTTTTAGAAACATTCGCTCAGACAAATTTCCATTGTTTGCTTTTACCACAAATTTTCCTCGATTTTCGCTGGCAGGTAAACATGACGGAGCAGAAATATTGCCAGTCAGCGCATCAGGGTGCCTCCATCGCGGGAGGAAGGCAGGTCCACCACCAGCAGCAGCACTTAGTGCGGGTGGACACCCCAGTGACGCCCCCACCTCCCATTCCGCGGCGACTACTGCGCGGCAAGTCCGCCTGGCAGGCGTCGAGTCCACACCCACTTCCACTCTCGGCTGGCGGACAGGATGGCGCCCACGGCACGGGCACGGGCACTGTGTTTGTTTATCCGCAGCCAGAAAACGTGACCGCAGAGGCAGTGGTCCGAGGATCGGGCGACGGACAGGGGGGCGGAGGTGCCGGGCTGCAATCAGCACTGCTTGTTGACATAGCCACACGTCCGGCGTCCGGATATGCGGATGCCGATGGGATTTCCGATGACGACCGCATGAGTCTGGAGAACTCCGTGTTCGACGAGTCGCTAACCTCCACGCCCGTCAAGGTCGCCGGCTATTTGGCCGGCCGCTATGCCGGGCTGAGTCACATGGCCAAGCGGGCGCAGCGTTCCTCCAGCAGCACCGTGGACTCCGCCTACGGCTCATCGCTGGGCGGCCACAGTGAGCGGTTCGCTAGCAGCTCGACCAGCGTGGACTTTCGCAGTCGCTTCTCCTCGGTGGACACCCAGTCCTCGCTCGATGAGAAGCCGCTGTCTAGCTCGATTGACTCGCCGCTGGCCAGGGATCCCAGGGATGCGTACAG

 

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