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CRIMIC CR01514

Gene 14-3-3zeta

CG17870

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:10099677..10109687	46E6-46E8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GTTCACTCCACAGAACATACAGTGCC	GTGCCTTTTGCTCGCTTGCCATACAT

Images

Construct Design Sequences

sgRNA sequence	CGGACAGCTGAGCTTGCCAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTAAACTGGCCGAGCAGTCA	CAAGGGCGGAGGTCTTTATACA	GTCCGAGGGTGTTGGTTGG	ATCGAACGCCTCCTGATAGG

 

Amplicon 5'

Amplicon 3'

CTAAACTGGCCGAGCAGTCAGAACGGTAAGTGGTTCATCTTATAATAAAGTTCATCAACCTCACAGAACTTCAAAAGAACCCTACAAACTCATTTGGCAAAAGTTATAGTTGCACATAATATTTATTAATACGTAAAAAAGACATTCTTGGAAGTTATAAGAACTCTAAGATTTAACATTGTTTTCTGTTTTATTAAATCCATTTAAAATCATCTTCTAATGAACAATATATGTATCATTTAAGTTGTTCTTCAAAATTTAAAGAATGCATATATTTCTGTGATCTACATGACTAAATATTTTCAATCACTAATGGAACATTTTCTATGCAGTTACGATGATATGGCCCAGGCCATGAAGTCCGTCACAGAGACTGGCGTTGAGCTCTCAAATGAGGAAAGAAATCTGCTCTCCGTTGCCTACAAAAATGTGGTCGGTGCCCGCAGGTGAGTCAATCAATCACAGATTGATTAATGTCGCACTATAGATTGGAGTTCAAGTGCTAAGCTGTATGTTTCTGCCCCTTCTCCGGGTGTGTTTAACATGTCCAAACCAGGTCATCGTGGCGTGTCATCTCCTCCATTGAGCAGAAAACCGAAGCATCCGCTAGAAAACAGCAGCTCGCCCGTGAGTACAGAGAGCGTGTGGAGAAGGAGCTGAGGGAAATCTGCTACGAAGTTTTGGTAAGTGGTGGTCCGCAAGTCTGCGCAAGTCTCTTTCTGAAGGATTTATGGGGTTGTAATTGGTCCATCTAATTATTTTCATGCCCACAACTCACTTGGTTGTTCATCTGCATTGACGGTGATGAAGATGGCAAACCTGTCAACCGCCTAAATATATATATATATATATATATGTATAAAGACCTCCGCCCTTG

GTCCGAGGGTGTTGGTTGGATGGTTCAGTGGGTTTTCCAGAGGGTGGGCCTGTTTTCTGGTCTTCCTTCTTGCTCTAGTGAAATATAAAAATTTGTTACAATCAATAAATCTATTTTTAAAATGAACATGTTCCATTTTCCCCCACACACAGAGGTTTTCGCTTGTGTGTATATGAAATTTATATTGCCGTTCACGGATCAGAAAATGACCATGGCCAGGGTTGAACTCGGGGGTTTCCCCATTCGCTTTTCTTTGATTGGGGCTTTTATATTGGATTAAACTTAACTGGTGCTTGATGGATTTTAAAATGGAAAATGATTTAGAACAAGGTTTACAATAAGCCTGGCGGCTTACTTGGTTAATTGTTGCGTTTGGTTTTCGAAATTGATATGAAATTAATTGGAGTACTTGTTTCCTTTGCTTACAGGGACTTCTGGACAAATACCTTATTCCAAAAGCCAGCAATCCCGAGAGCAAGGTGTTTTACCTGAAGATGAAGGGTGATTACTACAGGTATTTAGCCGAGGTTGCCACAGGAGATGCACGCAACAGTAAGTATCTTGTCAAGCACACCAACAAAAACACCCTATCCCCGAATTGTACGCCCGAATTGTACAATAATAAAATCTTTACAAACCAAACTACAAATACTCTTGCAAAACACAGCCGTTGTCGATGACTCGCAAACCGCTTACCAGGATGCATTTGACATTAGCAAGGGTAAAATGCAGCCAACACATCCCATCCGTTTGGGTCTGGCCCTTAACTTCTCAGTCTTCTACTATGAGATTTTGAACTCACCAGACAAAGCTTGCCAATTGGCTAAACAGGTTAATAACACACTGAGACTTGGCTCGATTAATCGAAATGTATGGCAAGCGAGCAAGAAAGGCACACAAAATCCCAATCCGTCGATATTAAAGAACTTTTTTGTTTACTCTCAAATGGTTTTCCTAACTTTTGGTTTTTATTTCTTACTTTTTTACATAACTAAATCATGTGTAAATCGTTAAGCCGTCGTTGATGACTCGAAAAATGCCTATCAGGAGGCGTTCGAT

 

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