CR01514

CRIMIC CR01514

Gene 14-3-3zeta

CG17870

Information

Donor Location Cytology FBti Stock number
pM37-p02R:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band GTTCACTCCACAGAACATACAGTGCC GTGCCTTTTGCTCGCTTGCCATACAT

Images

Construct Design Sequences

sgRNA sequence CGGACAGCTGAGCTTGCCAAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTAAACTGGCCGAGCAGTCA CAAGGGCGGAGGTCTTTATACA GTCCGAGGGTGTTGGTTGG ATCGAACGCCTCCTGATAGG
 
Amplicon 5' Amplicon 3'
CTAAACTGGCCGAGCAGTCAGAACGGTAAGTGGTTCATCTTATAATAAAGTTCATCAACCTCACAGAACTTCAAAAGAACCCTACAAACTCATTTGGCAAAAGTTATAGTTGCACATAATATTTATTAATACGTAAAAAAGACATTCTTGGAAGTTATAAGAACTCTAAGATTTAACATTGTTTTCTGTTTTATTAAATCCATTTAAAATCATCTTCTAATGAACAATATATGTATCATTTAAGTTGTTCTTCAAAATTTAAAGAATGCATATATTTCTGTGATCTACATGACTAAATATTTTCAATCACTAATGGAACATTTTCTATGCAGTTACGATGATATGGCCCAGGCCATGAAGTCCGTCACAGAGACTGGCGTTGAGCTCTCAAATGAGGAAAGAAATCTGCTCTCCGTTGCCTACAAAAATGTGGTCGGTGCCCGCAGGTGAGTCAATCAATCACAGATTGATTAATGTCGCACTATAGATTGGAGTTCAAGTGCTAAGCTGTATGTTTCTGCCCCTTCTCCGGGTGTGTTTAACATGTCCAAACCAGGTCATCGTGGCGTGTCATCTCCTCCATTGAGCAGAAAACCGAAGCATCCGCTAGAAAACAGCAGCTCGCCCGTGAGTACAGAGAGCGTGTGGAGAAGGAGCTGAGGGAAATCTGCTACGAAGTTTTGGTAAGTGGTGGTCCGCAAGTCTGCGCAAGTCTCTTTCTGAAGGATTTATGGGGTTGTAATTGGTCCATCTAATTATTTTCATGCCCACAACTCACTTGGTTGTTCATCTGCATTGACGGTGATGAAGATGGCAAACCTGTCAACCGCCTAAATATATATATATATATATATATGTATAAAGACCTCCGCCCTTG GTCCGAGGGTGTTGGTTGGATGGTTCAGTGGGTTTTCCAGAGGGTGGGCCTGTTTTCTGGTCTTCCTTCTTGCTCTAGTGAAATATAAAAATTTGTTACAATCAATAAATCTATTTTTAAAATGAACATGTTCCATTTTCCCCCACACACAGAGGTTTTCGCTTGTGTGTATATGAAATTTATATTGCCGTTCACGGATCAGAAAATGACCATGGCCAGGGTTGAACTCGGGGGTTTCCCCATTCGCTTTTCTTTGATTGGGGCTTTTATATTGGATTAAACTTAACTGGTGCTTGATGGATTTTAAAATGGAAAATGATTTAGAACAAGGTTTACAATAAGCCTGGCGGCTTACTTGGTTAATTGTTGCGTTTGGTTTTCGAAATTGATATGAAATTAATTGGAGTACTTGTTTCCTTTGCTTACAGGGACTTCTGGACAAATACCTTATTCCAAAAGCCAGCAATCCCGAGAGCAAGGTGTTTTACCTGAAGATGAAGGGTGATTACTACAGGTATTTAGCCGAGGTTGCCACAGGAGATGCACGCAACAGTAAGTATCTTGTCAAGCACACCAACAAAAACACCCTATCCCCGAATTGTACGCCCGAATTGTACAATAATAAAATCTTTACAAACCAAACTACAAATACTCTTGCAAAACACAGCCGTTGTCGATGACTCGCAAACCGCTTACCAGGATGCATTTGACATTAGCAAGGGTAAAATGCAGCCAACACATCCCATCCGTTTGGGTCTGGCCCTTAACTTCTCAGTCTTCTACTATGAGATTTTGAACTCACCAGACAAAGCTTGCCAATTGGCTAAACAGGTTAATAACACACTGAGACTTGGCTCGATTAATCGAAATGTATGGCAAGCGAGCAAGAAAGGCACACAAAATCCCAATCCGTCGATATTAAAGAACTTTTTTGTTTACTCTCAAATGGTTTTCCTAACTTTTGGTTTTTATTTCTTACTTTTTTACATAACTAAATCATGTGTAAATCGTTAAGCCGTCGTTGATGACTCGAAAAATGCCTATCAGGAGGCGTTCGAT
 

Search Again