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CRIMIC CR01515

Gene Spn55B

CG10913

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18127775..18127787	55B7	FBti0210415	86429

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTCTCCATCCAAACTTGCATTGCC	CAGTTCGTTGGCAATGCGATCCCATA

Images

Construct Design Sequences

sgRNA sequence	TATGAACGATTAGATATGTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCGTTCTCGGACAACACCA	AGGTGCTTGGAAAGGTGTGT	TCGTTCATAAATCCTCAATCGGA	AGGTGTCCTGGTTTCCGTTC

 

Amplicon 5'

Amplicon 3'

CTCGTTCTCGGACAACACCAGGCTGGTCCTGCTCAATGCCCTCCACTTCAAGGGCAGCTGGGCGCACAAGTTCAGCGAGGAGCGCACCGAGGAGGATATCTTCTGGGTGGGCGAGGAGGAGCAGGTTAAAATCAACTACATGAACCAGAAGGCCAAGTTCAACTATGGCTTCTTCGAAGATCTGGGCTGCACTGCCCTGGAGATGCCCTACCAGGACTCTGACCTCTCCATGTTCGTGCTGCTGCCGCAGGAGCGGACAGGTATTTACGCCCTAGCCGAGAAGCTCAAGACTGTGAACCTGGTGGATTTGGCCGACAAGTTGACCGTGGAAGAGGTCCATGTCAAGTTCCCCAAGTTTAAGGTGGACTACTCCCTGGAATTGGCCGAGAAACTGAAGCAGGTACATATTATTTTTTTTTTTTGGGGGTGGGGGGGGTTGATTGTTCCACCCAATTTATGCCACAATTAAGCTCCATTTGGGGTCAGCTTTTGTTTTTATATAACACAGTTCCTTGACCTCCATTTGATGTTGCGTGCTTAAGGTGAAGTTTGGAGTTATTAATAGCAGTAGCTCAATAAATTTCTCCATAAAATCCAGAATTCAATTTTATGGACACGTTGCAGTGCTGCAAACCGCCCACAAAAATATTTATTGCCCACTTATCTCTAAAATTCCGTCTATTGATGTACATATGTATTATTTTTAGTAGCTTATCGACGGTAGGGTAGAAATGTCTTAGATAACCAAAATGAGCTGTTGTGGTATTGCGGTGGCAGCTAGTGGCTCGAACTCAAGATCATGTATCAGCCGCACGATCCTAAAAAAAAATGATGGAATTTTAGCTGCAGTCGGATGTGACACACCTTTCCAAGCACCT

TCGTTCATAAATCCTCAATCGGATATGACCTCATTAGTTATTTACTTGGCACTTATGTATTTACCTTTTCACTTTCAGCTGGGCATCACGAAAATGTTTACGGACCAAGCTGAGTTTAGCAATCTGCTGGAATCCCCCGAGGGCGTCTTTGTGTCCAAGGTGCTGCACAAGGCCACCATCGAGGTGAATGAGGAGGGCACGGAGGCGGCGGCTGCCACCGGCATGATCATGATGACCCGCATGATGACCTTCCCGCTGCAGTTCCAGGCGGACCGGCCCTTCCTCTACGTCATCTGGAATAAGAAGAACATCCTCTTCGCTGGCGCCTTCGTTAAGGCTGCCTAAAAGCCCATTTCTCACTTCTTTCGTATTGTTTGTATTGTTGTTGGAAAAGTATTTTTAAATAAATAAGCGCTCCATGATTAAATTACCCGTGTTTATCGTATTGCCAATTGGAAATTCTTGGCAGATGTAAACAAATTTGAACTAAACACCTGTTTCAATAGAAAAATATGAGAACGTTGTTTGAGGATCAACATTTCGGTTATTTAAATATATGTTTTCATGATATTTGAAGTGCGCGCCACTCATTCAGCCTTGCCACCAAGTTCGTGGTCTGTTATCGATTCGATATCGAAAAGAAGTCGCGAAATGGCCTAAAACTGGCCGAACCGCAAGACCAGCCGAATTGAGTCTGCGCAGAGCGCGTAAAACCAAAACGAAGAGAACGCAGGAGAGTCGAACGGTAAATCGGTCGCAGCAACATAAACTTTTAGAATCGCCAGCGAGAGCCGTCTTAAAAGTGCGAATACAAACAGAAGGAAAAGCGGCCGCCTATGGCAACCTGACTAGCAAAACATTCGAAAGCAATTGGCCAAAGTTAGGACGAAATGAGCCGAAAGAACGGAAACCAGGACACCT

 

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