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CRIMIC CR01516

Gene PHDP

CG11182

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23883584..23883607	60A8	FBti0210416	86430

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TTGATAGGTTCAAATATTATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAAAATCCCTAGCGCGTTCG	TCGAAGCCAGCTTACAATCGA	ATCAACAGCAGATCGAGTTCT	GCGCCGGAAAGTCAGATTTC

 

Amplicon 5'

Amplicon 3'

CAAAATCCCTAGCGCGTTCGCCAGTTAATACCGCAGATAGCGGTATTTTCGTATTCGAAAGCATTGAGGGTATTTCGACTTATAATATGTTGCTTTTTCCGGGAACGATGGAATTGAAAATGTCCTTGGAATATTTGAATAAAATAATTAATTTGAATTCTTTTATTCTAAATGAAAAAACACATAAAATAATCAAATCAAATTGCAACAAAACTATTTAAAAAACGGAAGAGTACGTTTTATATATAGTTAAAAGGCTAAATGATATTTGTATATTTATGTATATATTTAATATCTGTATATACGTTTCACTAATGAAGTGTAGAAAAATGTAAAATGAACAACGATTTCACAGTTTATTAAAATCATTCGAGGGTGTTGAAAGGCGGGCACTACTTATAAATTGCAATATTAAGTTATACATCTGGGCACATATGATTTCAATAAACTGCAATTCAATCTTGATCTTGTTAATTAGATCTGAAAGGTATTCACCGCCAATCCCTGCGGTGGGCACTCCACAACAATTGCTATATTGAGCGCACCGAATCGCCACCATCAACTGATTACACGCCCTAATGCCGATCCAATCCACTTTTTCCACCCGATAATGGGATGACAATTTACCCCTGAAGGGCGTGAGGTTTGGTACTTGTAGATGGTGACCCTTCAACCCCCATTCAAGTCCTTGTTTTTCTTCCCACAGGCTTAGCCGGCTGAGCCCTCAAAAGGGTGCTAAGCCAGTTGTCCCGGATGATATGAAAACAAGCAAAGTGTTACACAGATCTTATTTATTTAACGCAATTCGCTCCAAATAGTCAGTAAAAATAATATCAAAATAACTGCATTCATCAAAATGTATACTAATATTTACATAGATATTAGTGGATTCTATATTTGATCGTATAAGCATTTCATTTGACGGCCATGGCCATTCTGGGGTCCCTTGAGAGATGGGCCCAGGTATTTCGATCCTGCTACAGGATTCTTCCGGCCATCCAATTTGCTCGACTTGTCCTTCATTATGTAAATGGCATGCCTCTCCTGCTTGCGAAACTTGGCTCTTCTGTTCTGGAACCAGACCTAAAACACACAATGGTTTTTTTTTTTTTTAATTTCGATTACTAAGTCGTTTTGTATCCTTCAACAGGATATGACTAAATTCGTTTCGATTGTAAGCTGGCTTCGA

ATCAACAGCAGATCGAGTTCTTTTCAATAGTTATATTATATTATGTAAGTATTATTTGTTATATGTTAATTATTGAAATATGTGTATCCTAAACTACCCACTCACTTAACCAGCAATTCAATCTCATTAGTTTTCCTCACCTGAACTCGAGCTTCCGTCAAGTGCAGCTTGGATGCGATCTCTTCCCTGGTGTAGATATCTGGGTAGTGGGTTTCCAGGAAGATCTTCTCCAGCTCGTTCAGCTGGTTGGACGTGAAAGTGGTGCGAATGCGCCTCTGTTTTGATTTGTCGGTCAGGCTCAAGCCGTCGTCTCCATAGGACTTCAAATGGAAGTCTATGGCGAGGTGAGAGTTGGTTCAAGATTACAATCCATAATCGATCCATCCAGGTGCACTGGCTGCAGGTGGAACTGATGGCCACTGTACTTACCCGTGTTGTAGGAGCCAATGTTATGGGTGGCCGGGTAAAACTCGGAGACAGTGGTGATTTTGGAGAACAATAAGCTCGACTCCTGGTTGAGGGAACCCCGGATTGCGGACTCGTTGGCGCAGAGCTTGTAACTGGAGTCAATCATGAGGTTGTAGTGATTTATGTGATTGGCCACGGAGAGTCCTCCTCCATTGGCGCCGCTGTTGTAGAACTCCGAGTTCGCGGTGTACAAACAATCCTTGTCGAAGTTCGCCTTGTTGAGCAGTGAAAATTCCATCGCATCTGCAGGTAAATTCACACACGGCACTCGATTGAAATTTTACAGCCCGCTAATGAGTCATTCGGCCATAAAACACATAGTTATAGTAGCTCCGCGGCCGCACACACTTTTAACTGCCCTTGAATAAATAAGCTTCGATTACGATTGTATCAACTACAGGACATCCACGTTGGACGAACAAGGATGCTGCACTTGTCTCGGGAGAGCGGGACGGAAAATTTCATTCGTTTCCGCGTTTGGAGGCGGTCCTAACGAGCTCCACTCGACATAGGGGTGGAAATCTGACTTTCCGGCGC

 

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