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CRIMIC CR01520

Gene CG13920

CG13920

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:1648944..1651501	62A9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ACTTCAACGCCAACCACAATCCGTAC	AGCATCAACACCAGCAGCGTAACGTT

Images

Construct Design Sequences

sgRNA sequence	GGCCAGATTCGAAGGGGCGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCGATGAACAACGAGCCCA	GCTGTACCCCAGGACAAAAG	CCCCTTCGAATCTGGCCTT	ACGTACTCTGTGCGCTGAAA

 

Amplicon 5'

Amplicon 3'

TTCGATGAACAACGAGCCCAGCAGGAGGATGCTCAGCTGCAGGAGCAGATTCAAAGCTACAACCAGCGCATGGCTGAGTCCAGGAGAATCTACCACACGCCCCAGACTCCACCCGAGGATCACCATCACAGAGGTGGGCCTGGTCTGCCTAGAGGACCAATGGGATGGTAGTGCTATATATTACCCTCATAGCTTTAAGTAACATCTAAGTAAATCAACAATAAATATCTGAAACCCATTGATCGTGCTCTTATAATACTGAGATTATGTTTAATACACTAATGGTAATATCTGCTCTCAATTACTAAAACTCTCATTTCACAAGGAGTTTCCGAGTTTTGTGTGTCATTCATTTGGCGAAAGCCACTCATGTTCACCTAATGATTGTTTATCTGGGCCTCCTTAAGGGTTGCTAGCCGGGGTCTTTGGCCTCATTTGCGCCACTTTCGGAACAACCACCACCCGAGCCGACTGCCCGAGGATGGTGAAGTGGGTCCAGCTGCCGTTCCGCCATTCCGCCGTGCTCTCCACTCTCCGGGGTGGGCCTGTCGCGGCTGTGGCTGCGGCTGTCCTCTCTTGACTCTCGATGCGGCCAGAGCACCGGTCCCGTGAGCACGTATTGATTTTCCGCCATTCCGTTTGGGATGCTGCCGAAATAAACTTTGCAACTGCTCTGAATGTGAATCTGAATCCGAATCCGAGTCAGTCTCTTTCCGAACTGAAAGCGGAAAGGCGTCGAGTGAAAATGGAATAGGTGAAGGGAACGCCGCCGCGGACTAAAAATTCATAAACTTATACATACCGCAGCGATTTGAAAACTTGAAAACATTCGGATTGTGTGCCTCAATTTGCTGGTGGTCGCGTATTTTTCCGCATCGTCCGAATCGGCACGAAAAGCTAAGAGATTCGTCTAAAATTAGCGACCGGTGTGTTTTGGTAGGGTGTTGTATTGGCTAGGGGCTCCCAGTGAACTCCGCCCCTCCGAGTATTTACCCATAACCGGGCCAAGATGCCTCCTGCATCCAATACGATCGTGCTGAAGAGCCTCTCCGTGCTGCTGGGCCTCTTCTTCATCTTCGTGGGCACCCTCAAGCTGACGCCGCACATTAGCAAGGATCTCTACAAGGACCTGGTGAGTGGAAAATCGATAGCGCCAGAGTTTTAGGCCTTTTGTCCTGGGGTACAGC

CCCCTTCGAATCTGGCCTTTTGAAAACTATGTGCTCGCCCATGGGAACGTTCTTATGTAAATGGGCGGAGACAGATTTCAAGAGGCGTCCCATTATAGAACGCCATTCGGAAATATGACTGACTAATGGGCATTGAACTCGACCCCATCAATGCCAATCGCTGACATTCGGACATCGCGACGCGCTATGATCTTCAAAAATATATTTCAGGAAAAATATATGCTTCAATATCGAAATAGTCTCCCAACGAAATTCCGAAAATTTGGATTTCAAATGATTGAGGAAGCACTTTTGAGCTTTAGCTTTGCAATTATCCAAATAGATTGTGATTCATACAAATTGGACAAACATTAATTTTTCAATTTTTTTTGATGAAACTTGTGAACAAGTAATCTTAAGACTAAGATCTGAAAGAGAGAGAAACCTCGAAAATTTGGCAAACTAATTATTAATCACCTTGTTTGTAAATTGTTTTGAAAACCTCCAAACAAATTCGAAACTAATGATATATAGAACAGCTGTTGCTCTGAATTTTTTGTTTACCTCTTGTAAAAATAACGCCCAGAGATTTTCGTTTTCATTACCATAAAAATAATTCGCATACCAAAGTAAACATTTGCGAACACAAGAACCACAATGACGTGTTCTTTTCACTCAATTCAGAGTATACAAATTGGTTTTATATACATAAACAATAGGGTGGCCTGAGGGATTATTCAAAAAACTTTATAGTTTTAGAGATAAAAGTAAATAAATAACCAACTAGATCATCCAAAAGACACTTCAGTTCAATACTTTCGAAAAAAATCAGTATTCAGATGGCGTTTTCGAATTGGCATTGCATTCGTTACGCGCCATTTCACTTTCAATTTTGAAGATTTCTCAATCTCGTTTTTCCCGAAGGCATGGCATTTGTATTTCGGTGTAATTACCCCCGTATTACCACAAACACTCGGGGCCAACGATGCCAACAGAAACAGATGAGTGTGACAACAGGGGAAGCTTGCTTCCATTTAAAAATAAACCCGCTGCACTGCCTCTTCCGCTGGGCCGCCAGGCGGAGCACATTCCTCTGCGGGTGTTTCTGGGCATGATTAATTATATCCTCGTATTCGATTTCCTCTCGATTTCAGCGCACAGAGTACGT

 

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