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CRIMIC CR01524

Gene Idh3b

CG6439

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:22016391..22019382	93F14-94A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGTGTCTATCCAGAAGATGCATTGCG	GTCGTGTTGTCCACGATCATCTTCTC

Images

Construct Design Sequences

sgRNA sequence	GTGCGAATATTTCTGCGCTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAATACTCGCCCTCCGTCTG	CTGAGGCCTAGTTCTTATACGTT	CGTCGCGAGCAAGTGTTTA	CCTAAACAGGGCCAGGTGAG

 

Amplicon 5'

Amplicon 3'

GAATACTCGCCCTCCGTCTGCTCGCGAATAATCACCGTGTCGATGTTGGTGTGGCGGGTCTTGACACCGGGCAAGCTGCGCACATGCACCACATTGGCGTAGAGGTCCAGGTCGTTGCGAAGCTTCATGTTAAGGGTCTGCAGGTCACCCACATTGCTGTAGTCGGGTGTGGCCAAAACGCCCTGAAGGAGCACATTGTTTAGATTTTATCACACTATTTTATTTTCTTGCAAACTTACCTTAATGCACACTTTGTTTTTCTGAATGGATGCCACCACATCCTCCAGTTTGGCACTAAGCACGGGATTGATTTCGGAAAGGAAGTAGCACTCGAAGTCCACGGGAACGCTGGCTGCCTGAACGACAAGATAAGATAATTATGCATATTGATTGGGCTTGAAAAGTTAAAGAATTTACATAGCATTTTTTAATTTAGTCGCACGTATGAACATGTATGTTCAGTTAAAACGGAAATCCCGAAATCTACTAAAATTTTCAAGTAGCAGCCACTTATCACATATCTTTACGATACAGATAAGTTTGATGTTTTGATTTATATAATAAAAACACTCGTGATCGGGAAAAATGGAAGTGTTATCAAATACAATGCGCTCTGTGAAGAGTGAAATATATTAATTAATTTTTAGAGCCCTGTGCTTTTTAAATCTAATAGATTCTAGAAATATGTACATAACTAACTAAACGCCGACTTGCTTAAAAAAAGCTTTTCTATTAAATAGGAAACCAAGCTGGCAATGATCCTAAGCTTAAATAACCTTTTTTTTAAGAGAGATCAAGAGACGCAAGAGTATAGATTTATAAGAATTAGGACGCATTGGAATACTGTATGTGAATGATTGCAACTTGATGGATGGTTAGCTACCTTGAAGACCTCCTGGAGGGAGTAGACCAGCTCGGGACCCACACCGTCGCCGGGAATCAGTGTGCAGGTGGTCCTGTTGGCGCCGTAGTTATCCTGCAAGACCAATTATTTTCCGGTTACAACTGGTGCCACTTAATAAGCGCATTTAACACTCACCGTAGCCCGTAGCGTCGACGTGGTGTGCAGACTACGTGCTGCCGCAGCCTAAAACGAGAAGAATGCAATGTAATCGTTAAGGCTGGCTAGGATTATAACCTCAAAAAAAAAAAAAAGAAACGTATAAGAACTAGGCCTCAG

CGTCGCGAGCAAGTGTTTATGTAAGAGCTGCGGTTTGGATGGCCGTTTACCTGCATGAACGTCCGTCCAACTGTTCTCGCCAGCATCGACATTTTGCTATTCGGTTAGCTAGATCTTCCCGGCTGTTGATCCAAACTCAAAAGTGTGTCCTTTGGCCACGCGAAATTTTTGATTGCGATGTGATTTTTGCTCCGGTGCCAAACCGAAAAATTTCAATAGGACCAAATCGACCACGCAAACCAGCTGATTGCCTCTGACTACCAGGGCTGGCGATAGCCGCCCACGAAACTGGCGCGTCCAGTGCTGGCTAACGACCAACTATCGCGATTTTGGCGTTCGAAAATTAGTAACGAACATATACGATTATTTAAGGAATAAATTTGGCGGCAACCGCATAGTAACCTGTTTTTGAATACATTTGCCTTGCCTACCATGCTTTCTTTTCAGAACAGAGAAATTAAATTTAGCCAATGCATTTTATTTGAATTTTGACATTCAGCATACTTTTTAGTCAACTTATTTATTATATTAATTTTTTTCGCTAAATTAAACAACAAGTGGATGTTCTTTATGTTTATTTATGGCCTAATCACATTGTTCTTCAGAAACACAAAGCTTGCCAATTTCAGCTTTAGTAATATATCATATTCCATTCATGCCTTGGTATCGAATTTTTTACAAATCTTCCCAGAGTATTTATGTTTTGGATTTTATAATGATGGTTTCTATGTTTAATTTGTATAATTGAAGCTAAACAAAATGTTACAAATATATGTATATAAGTGCTATTTCGTTTCAACTGTGATCGCTAATTGGACAAATTACATATTGTTGGTAATCGTACCATCTTCTCCTGGACTAATAACAAATAGTAAGTGTATCATCTTGTTTCTGAAGGAAGGGGACACGGGACGTAGTCTACAAATACAATAGTCCGCTGGCAGCGGCGTGAGCCATCAAGGTGTTGGCCGCCTGCTGAGTCTCCAGCATGAGGCGAGCCTCCTTCATCCACTCGCCGGCAATCTTGCGCGATGCACCCTGCAGCAGATTCATGTACTTGAGCGCCTGCAGAAAGTCGCTGCGATCCACATGGTACCTGTGAAACATAATATATATAATATATATTATATAACTCTAGTGATAGATGAATCAATCGCCTCACCTGGCCCTGTTTAGG

 

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