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CRIMIC CR01527

Gene CG11155

CG11155

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	4:1126602..1137919	N/A		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGAACATTCTTACGCCAAGGATCTGG	CGCGATTGCATTTCCACTAGCTCAGT

Images

Construct Design Sequences

sgRNA sequence	GAAAGTATGACAGGCAAAACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGACGGAGCCTCGGATGATA	ACGGGATTATACAAGGCATGT	TTGCCTGTCATACTTTCACGA	GCGGCTTTAGGGGGTAAGAG

 

Amplicon 5'

Amplicon 3'

TGACGGAGCCTCGGATGATAATACGGTGGATTTAGTTCGAATCCAAAACAATTTTACTTTAAAAAATAGTTTTTGGTTTGCCATTGGGGCTCTTATGCAACAAGGTTCTGACTTATATCCTAGGGTACGGACTATTCATTAAATCTAAAAGTCACTACAAAAAGAGTACCAACGAGTAACAACAATTTAAAGACAGAACGAAAAGTAATTTTATACATTTGTGGTCAAAACTATTTACAGAAAATGCAATTTGATGCAATTTTGATGCAATGTAAAATTAAAAAAAAAAAATAAATTGCAGGAAAAACTGTGTATACTTTAAAGACAAAAGTTACATTTTGGTTGCTATGTTTCGTTCATGATTATATTTATATTCCGTTACTCGTACAGTGTAATGGTTTAACTTTGATTAGATTAGTTCTATGTTATTTAATATGTAAGAATGATTATTTCCGACTCTATAAAATATATATTTTTTTACCAGACGAGTTGATCTAGCAATGTGCGTTTGTCCGAATGCTGAGATTTGTCGAAGTAGACAGAATAATGAGAAGTACAATTTTTAGGCGTAAGAGTTGGTCTTGCATTTCAATACAAACTGGAATGAAAAATGATAAAATGAAACCATTAACAAAGTGAGAACGCTATTGTCCATTTTCATTGTGGGCGTGGCAGTGCGATACAAATGGGCAAGACAAATAATAAAAATCTAAACATATTTCAATAATGTGGACGCAGATTGTGGCGTAGCAACTTTAACTGCGTCTATGTTTATTTAGTTTTCATAGTTCCTGAGAAGAATGCGTTCATAAGTAGACGAACATGGACCCATCGACTAATGATAGTAATTGTGTGCAATATATATACATGCATATGTACATGCCTTGTATAATCCCGT

TTGCCTGTCATACTTTCACGAATCTAGCAAATCCTTTTATTCTGAGAAGAACGTGTATAATTAATACATTTTTTAAAATTTTTCGTTGTCCGTTCGCGCAGCATGTGGCATTAGGGAAAAGGAAACGGTTAAGAACGGTTTTCCGAAGTGCGGGAACTTTTAAGTTAGTACTGAATCTGAGTCGACTCTTAACAATCTAGCGCAGTTCCCGAGATATTTTATCATATACATATCTCAAATCTTAGCATGGACGTACAAACGGTGGACAGGCCCAGATCGACTCGTCTGATCAAGAATATGTACTTAATATGGTCGGAAACGCCTTCTACCTATTACATACTATTCAACAAATTAAGTATACCCTTTTTCTACTAGTAACGGGTATAGGAGTCATTAGTATATTATACTAGTTATTTGTAGAGTAAAAGGGTAATATAGTCGCGATATTCAACGATACTGTTTTTTTTTTCTGTAACTAATTATATTTACAAACGCTTTCAATCCGAATACTTTTTTGTCTTTTTAGGCAACCTCGACGCGTATTGTGGGCGGATGCTGGTTCTTTTTTTGCCTAATCATTATATCTTCATACACTGCCAACTTAGCTGCATTTCTAACAGTTGAACGGATGATTTCCCCCATTGAAAGCGCTTCTGATTTGGCAGAGCAAACCGAAATATCATATGGAACTTTGGAAGGTGGATCGACAATGACATTTTTTAGGGTAAATATTTCAAACAAAGGACATACGCATTTTAAGTTTTATTTGCACAACATAATAATATTTTTGCATAACGTTATAGCACAATAAATTGTGAAACTGGTAAAAAAAATGATATGACTAGCAAAGTCAAACTTATCTTGCTAAGAAATCGAAAACTGAAATAGGCAAAGAATGGGAGATTCAACATACTGAGTATAAAATGGCGGTGGTACACAAGATTGTATTAAAACTTTGTAATACTCGAAATGTACAAAAAGAATTGTGTGCCTAAATGGTTTTATTCGCGTCGCTCTTGGCAATGCGGAAATTTAGCTATGTATTTTTATACCTGTTATTCGTAGAGTAAAAACTTTTTGCCACGCCCACTCTTACCCCCTAAAGCCGC

 

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