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CRIMIC CR01530

Gene wapl

CG3707

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2153065..2153067	2D5	FBti0210418	86432

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAATACCAAGCGCCCAAAGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCCTTTGACCTAGCAGCTC	TGGGCGCTTGGTATTTGCAT	AGGCGGTTTGGAGCTCAATA	CAGGTAACGCAGGTGCTACA

 

Amplicon 5'

Amplicon 3'

CCCCTTTGACCTAGCAGCTCGGCTCCCAACGATGGCTGCGCCTCGTTGAACGTGTGCGTCAGGTTGATCAGTACCTTGAGCACCGGCACCAGCAGCTCGCGCATAGCCACACCCGGATGCTCCTTGTTGCTGCCACCACCATCCGAAGGATGCAGCATTATTTGTCGGCTGCAAAGACGGTACAGTTGGCAAAGCGTCTCCACTGCTTTTCCCTGGCCAGAGGTGAGCATGTAGCGCTGGTTCGTCTCATTGTGCTGCGTCACGTTTTCGAGGACTCGCAGACAACGCGCCACCGTTTGCATGTTATCCAGCAGCGTCGGCTGCCAGTCAATCTCCGTGTCGCAGGCAATCACCGGTCTGCAGAAGTCCGAGATGGTCTTGATAATGTGCTCCAGGCCACCCAGCTTGCGCAGATCCTCTTTAAACCACTCGCCCGCGCGCTTGGATGTTAGCGATAGCAGCGTTTCCATTGCCAGCGTGCCCACAGTCAGAGAATCAACGTTGAGATGCGTTCCCTTGCCCTGCGCCTTGATCTCCTCGCACAACTCGCGCACCTTCTGCTTGTTGCGGTCGTAGCCCGCTCTATCCGGGTGCCCAGTCTCCGTGCTGCCACCCACGCCGTCCGCCTCCAGAAGGTTGATCATCAGCTCCAAGGAGTCGCGATCCAGGTCCATGTTGAGGCCCTCCTGGGAAAGAATGTACATGATGGCCGAGGTGCACAGGCCTAGGCTGAGGTCCTTGTTGGCGTCGGATAATGCCTACAGTTAAATAGTCAGTTAATTAACAAAGAGGTTGATACTTTTCCTAAAACTTTTTTTTTTGTTATTCACTTTGCAAATAATCAATAAACTGTACTGATTTAAAGTGGAAAACATTACCAAGTTGATTAAAGTACAACTGCAATGGTTTAATAGTTTATACAGGCTTTTAATACAGAATAACAAAATAACAACACAGCTTTCAAAACAAACTGGCTATTCTGGGGACAGCTTTGCCGAAAGCTGATATTTTGAATTTGATAAGATTCAATTGCATAATCAAACTATGCAAATACCAAGCGCCCA

AGGCGGTTTGGAGCTCAATATATGTAAGAATTGGGGATCTAACTTACCTTGAAAAATTTGGTGACCACCCCATGGGCACGCACGTGCATCCGGAAGGCGGGCATCATACACTTGGCGGCTAGCTGAAGGGCGGAGAGACACCGTGTTGCCGGGGGATTGTGCGGCTGAAGTGCGTCCAGGATGTACTCGACGTCGTCGTCCATTTCCTGGTACTCGCCGATCTCCTGAATTTGATGGGCCGTCTTAACGTTACGCACCACTGGATAGTAGTCCTTAGTTTTGCGGTCGACCCGAACGGGGCCGCCACCGCCCGTCGCTCCACCTCCGCCTGTCGTTGCCCCTGTTCCACCAGCGGAAGCACCAGCTGCACTACGCTCTAGGTCAAGCTCTCCGGCAATTGGTTCCAAATCCAGGTCAAAGGCATCGGAACTAGTGGAGGTGGCAGGTACTCCCTGTTTTCCCGCCAGTCGCGAGATCTTTCCGAGGGCCGGGCTATCGCCGCGCAACGTGGATGCGCTGCTGCACGCACTGGAGGAGCCATTGTTGTTCGACGACGGACTGTCACCGAAATCACCATCGGACTTGCCCGCCGCTAAATGATGCAGATGATCCTTCGCCCCAGCGACGCCCACGCCAGATGCCGAGGCATTGCTGGCATCGCTGTTGGTTCCACCGCCATTCGCTTCTAGCGCAGCATCCCAACTGTGCTTGTACAGATGTCGCTTGGCCACTGTAGCCTGTTCCGCTTGATCGGATGGCACCAGAGCGCGGCTTTTGAAGATGCTGCCTTTCTTCTTTGAGATGACCAGCTTGACAGCGCCCCGCTGTTCGCCAGAGGACTCAGGTGGCGCCGCCGCTGCTGCTCCTCCCGCTCCGGCGTTCGCCGCGCCCTGTGTGTCCAGTGTCAATGTTCGCTTAAACTTATCCTTAAACTTGAAATCGCGGGGTGGGGGCGAGGAGGAGGACCCATCGTGCTGCAACGAGGAGTTGTTTGAGTTGTTCGACAGTTCATCCGGATCAGGATCCGGATCATGGTCTGGCGAATGTTCCCTGTGATCTGTGTGCGGAATCTTGGCGGGTGTGGGCTGCTCCTCCTCAGCATCAGGAGTTTCCTGCTCTTCGGGCACTGGCTCCTGCTGTAGCACCTGCGTTACCTG

 

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