• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01531

Gene Myo10A

CG43657

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:10958391..10958396	9F13	FBti0210419	86433

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACTGGAACCGGGCTACCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGTAGCCTCTGTGTCAACA	AACTGGGTCAGATGGCCTG	GCCCGGTTCCAGTTCTAGTA	TTTGGCCAGCAACGTGAAAA

 

Amplicon 5'

Amplicon 3'

CGGTAGCCTCTGTGTCAACATTGTTAGTAGGCAACAAGAAGGAGTAGGGAAATCATCTTTTTCGGGCACTCATTGAATTTCCCAATGGATCAGCAGTCTTTTCTTTCTTTTCCATACCAATTGATATGGTATATATATGATATATAGGTTTGATTTTAAAGTTAGATCTAGTAGGAATCCGACGTTTTATGTACCTATATCGGATGATATCCCCTAAAAGTAGAGAATGTTCCGAAGTCTTCGATTCACTGAATAGTGGTTTTAATGAATCCCCTGATTGTGTTTAATCGATCTTGTCCAACTGGAAGAGGTTGAATCGAGTTGAGCACGGGGAATGGGCTGGGATGTGCGTATTGCACGTTCTAAATTCCCTGGCGAACTTTCTTGCCCCCGGAAAATGAGGAGCGTGCTGGATCAAAAAGATGCCATCACGTTTATAGTTCGGGTTCGATGACCACTCGGTGTGCGATCCATAAAAACACAAGATTAAAGACAATGAGAACTGCTGAATGGGATGGCTTGCTTGCTTGGATGGATGGATGGCTGGATGGATGGTGACCCACAAAGATGCAGGCTGTACGATAGTACAACACGGGTTATTATACTTCTATACGAACGCCATGAAAAAAACCGCATTATAATCTAACCGTAATTTGATTTCAATAATTTAATTACACTCTTTTCGTGTGTGTGTTTGTGTGATTTTGTTGTTGTTTCGTATTGTGCGTCTTTATTGCTTTCGAGCTGCGCCAATAGTAAATTACTTTAATTTCTTTCGACCATCCGCTGCAGTTTGGTCCGCCGTAAATCCATCCAAAACGGACGGACAACTGGCATTAATTTCGAATTTTTAATTAACTAAAGCTCTGGCAAAACAAAAGAAATACCGCGGCACATACGATGAAATTCGTATGAAATAACACAAAGCCAGTCAGTCAGCTAGCCAGCCAAAAAGAAAGTTTTAGCGGTCACATAGAAATTACAGTCCAAATTTAACTCTTGTTTTTGCCCACCACATGCGTGTGCGAGTGCTTTTTGCTGTGACTGTGCGAGAATTATCGCGCTGCAAGTCGAGTGCCACGGTAACTGGAGCATCTAGAGTCCACCGATTCAGGCCATCTGACCCAGTT

GCCCGGTTCCAGTTCTAGTATGCTACAATATAGTACCATACCCACATACCCATTTCTGCTGTTAACATAAGACTTGGCCCCGTTTTTTCGTGTTTTTTTTTTTATTTTTTTGGCTGACAGCTAACAGCTAACAGTCTTCTCGATTGCATCCAGACAGCCAGACGAGTCCCCAAAAGCTTCTGGCCGGGATTAGGCACTTGGACGTGGGTGCTCCGCTTCCCAAGGCGATCTCAATTGGATGTCTAGCACTTAACGATGAATCCTGCTGAGCATGTTGGTGCACCCACGCCATTGATGAAGTTGCATGTTTCTTAGCTCTGTCTTGGTAAAGAAATTGTGTCACTTGCAGATAGTTGATTTTGTTGTTCATTTTTTTTTTTTTTTTGGTAAAGAGTAATACCGTTCTGGAGTATATTAAGCAACATAACTGGCCCAAAGTTATTGCACATGAAATTAACCACAGAAAAACAGAGATGCGTGAAAATGTATGGTAACTCAACTTGCTTTCGTTACATTACTTTTTGTGGTTTCTAGACGCAGTTAAGAAATCTGAAAATAGTGACTGGGAAATGGCCGAAAATCATGCATAATTTATGAACTAAAAACCGCTGTTATGTTTAGAAACCTGTTGGTCTGCCATGAAATATTGATAGCCATCCGAAAATGAAGAAATGGAAATGCTTACTTACTGGTTGATTGACTAAGCGAGTCGTCAGATTGATTTTGGTAGCTCTGGAAATGATATCACCTTTTAATCCACTTTTCCTCTCTCTCTCTCTTTGCATCTTTGCATCTTTGGTGCATTTTAGTTTGATTAAAAAATAGTTGCAATACCAAAATCAATCTTCATCTACAACCAGTTGGGTGTAATGATGAGTTCGTATTGATTTTATGTGTGTTTTTTTTTTTTAATTTTCCTGCAGTGGTTTTTCACGTTGCTGGCCAAA

 

---

Search Again