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CRIMIC CR01532

Gene FucTC

CG40305

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:23009702..23009706	20F3	FBti0210420	86434

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGTGTGGCATTGTCATGCTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCACTTAAGGCAAATGCGG	GACAATGCCACACTGCACAA	CTGCGGGGTCATGAAGCATA	ATCACTTAAGGGGCCAGCAC

 

Amplicon 5'

Amplicon 3'

CCCACTTAAGGCAAATGCGGGAAACTCAAAGTGCGTCTCTCTCATTTTCGATTTTCCTGTGTTTCTGGTTATCTATCTCTTTTTAATGCGCGCACAGTTCACAAACGTATTCGGCTTTGCAAGCGGCAACTGTTGAACCACCAGGATTGGATATCGTGGTAGAACTGTGTCTTGTGGCCAAATGAGGGATCATGGAGCCTGGCGCAAAGGTCGCAAAACGGGGAGCTGTAGGTCAAGCGGTAGTGGCTGCGCCACCAGAAGTAGCTTACATACAAATCCGGATCTGCAACCACAAGCTTCATATACTGGGCCAGACCTTCCACGGACATGAAGCGATTAGCATCGACGTACGAGTGCGGAGGCAGGATGCGGGAGTAGTCTGCTCCGCCAAAAACCACGGGTATTACGGTCCGTTCTAATGCGTCGAAAAGTTTCTCCGTCACGTAATCATCGCACAGCGAGTTCTCAAAAGCCAGGTAGAAAAAGTAGTCAGTGTCCAACATCTCGGCGCAGTGCGGATCCCCCCGGGTGCAGCTGAAATATAATTAATATTTAGAAAGTAAGCATCTGCGTTTAAAAAACAAGCAGTTTGCATGCCTACCGGTTGCTCCCAACTTAAGGCATTTCATTAAACTTGCGTTCTTTAGTTGCAAGCTAATGAGATACGAGTAAAGAATTTGCGACAAATAAAAACTTTCCATCAAGCGGCAAAACTACCAAAATGAATTATCTGAAATTTCTTAGACATTTTTTTTAATATACATATGCACATAAAACCACAGGGAATAACAGCATCAGTTTCTGCTCAGCCACTACATTTAATGGCTCTGCAGGCTTAGCGAGCGACTTTAACATACTTTTGTGCAGTGTGGCATTGTC

CTGCGGGGTCATGAAGCATAAACTTTCACGAACTTGAGTGCAGATATCATAGTCTAAAAATATGCATATGCTTTTGGTATGTACTATTTAAAACTAATTAAGTTTTTTAATCATTTACTTTTATTGAGAGCTTAAGAAAGCTGCATGTGCATTTTGCATTATAATTTTGTTCAGCTAAAAGGTCCTCACCTGAGTGTTCCGCAGTTTCCGTAAATGTCCACTTCAAAAAATTCTTGAAGCCTATTGGCCAAGACCTCCCTTTTCGACAACGTTTCACAATGAGAGACGAACCAGGCGGCCGTTTTCGTCTTCCCCGACCAGAGGTCCCAAACCAGAGAATCGTTAAAGGCCACAGGAGGCTTCCTCCAAGGTGGTTTCACACTGGGCGCCACCACGGCGTCCGCTGTGATGTCCAGCAACTGCCCGTAGGGCCAAAATACATCCGAGTCAATGCGATAAGTCATGGTGAGGTTATAAAATCCTTGTTCGTCGTCCAGCCGGCTTAGTCTCCCCAGGCGGTTCCATCAATGCGAATACGTAGACCTGGTGGGGGCTTCTCTGGGAGGGCACTGGTCGCAGGAGCGGAAACATCTCCGCCGCATGGAATACAATGGCATCATAGAGTTCCACTGCGGGCAGGAATGCGTTTTGGTTGCTAATCTCGCACTGATAAACAGGACAGTGGAGCTCATCCCGCAGCTCCTGCGGTCCCAAAGTGTCCCAGGAAAGCTTCCATCGCGAATCACCAAAAAATTCTGTCCAGAGGAGAATGGTACGGGTGGATTGGATATACCGCTCGTGCTGGCCCCTTAAGTGAT

 

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