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CRIMIC CR01534

Gene r

CG18572

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:16662681..16662693	14F6	FBti0210421	86435

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTTAAGGCTATCCACTTCAGGAGA	AACTGCACACCTTCCTGCTGAACCAA

Images

Construct Design Sequences

sgRNA sequence	ATGGCGCATTGTCTTTCCCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGATGGATTTCCTTGGCGCT	CCCAGGCGGAACAGTTTCT	CGCCATTCCAGTTCCCTGAA	TTCGAAGAGTCCACCACAGC

 

Amplicon 5'

Amplicon 3'

TGATGGATTTCCTTGGCGCTGGCGGAGATTCAACCACCGTGGAGGGACTGCCGCCCGATGACGTTGGTATATGCGATTCACACATGCGTTCCATATCGCTGGCTGCGTTCTGTAGTTCCTGGACGAAGTGCTGTGCCTGCTGCGAGCAACCGAGGTGCTGATAAAGGCGGCACAATCTTTGCCCATGCTCCAGATAAAGCTGTGCATGTCCACGGTTGCCAAACCGAATGGCACGAGCCAACATGGCCAGCGAATGATCCAGCAGAAGTTCGATCAGATTGCTCCTTCGCAGGATCTCCAAGCCGTTGTTCAGTTGCGGTTTCGCTGCTTCCTGGCTCTTGGAACTTTCCGGATTGGAGCGTGCCAATAGCAATTGGCGATCCAGGGAGTCGAGCGAGCAACGTCTTTGTTCCGTCGCCGAGATTTGAAATTGCTGAGTCGTGGCCCTTAAGAGCTGTGTCTTCCTGCGCAGCTTTCCTCTCGAGCGCTCCTGGTGCTGAGCCAATCCCAAGAGTCCCTTGGCATCGGTGATCTCCGTCCTTTCGGTTTTTTTTTTCGTTTCCACCTGCTGCCCGCCATTGTTTTCTTCACAGTTCTGCAGAGCGGGAAATCCCTGACGTGGACGCGAGAACTTGCCCCGTCCGTGCAGCTCCAGGTAATCCTGGCTAAAGAGATTCTCCAACAGTCGCTTGTACTCCAGTTGCGGGTAGAATTCCGGCGCGTGCTGAAGATTCCTCAGTGCCTGGATGACATGCAGCAGCGGTTGTTCCGCAATTCCGGCCATTATGCTGCGCAGCTTCATCAGCGTATCCTCTGGGATATGCTCGAATAGATTGCGCCGCTCAATGAAGTAGTCGCTCAGGTGTTTCTTGGCCAAACAGGCGTCGAAGGAGCGAATGAATCGAACCAATCGCTCGCCCAGAAACTCCTCTGGCCAATCATTGCTGTGCTGCTCACATTGCCAAAACATATGGGCTCTCAGCTGTTCGAGCAAAGCTCCCGGGGATTGGTCACAGGATGTGGTTGATTCGTCCACAAAGGTTTTGACCAGCAGCTTCATTAGTTGGAACACCTTCAATTGCATGGGCGTCAGGCAGTGCCGCTCGAGAAACTGTTCCGCCTGGG

CGCCATTCCAGTTCCCTGAAGGGATTTCGCGAACGCTTTGGTGCATAGCCCACTGGTACAACATGGAAGCCCATGGATCGAATACGTTTTTTCATCTGCTCCGTTGGCCATTGGAATTGTTCGGCAGTATGGAGGTTGGTCAAACGAGGACGAGCCCTCAGCCAAAACTCGAAGGCGCAATCCGGCCAGGAATTGGGCACATGAATGGCGGGCACCAGCTCCTCGCGAGCGGTATATACACTACAGCCGCGATACGAACACGCGGCCAGCTGCGATTGGCTGATGCCCAATTGGTTAGCCAAATGATGACGCAGCACCTCTCCAAAGTACAACATGAACTGCTGCGAACTGAGGTAGCACTGCTGCAGTTGCTCCACATGACACTGGCGAAGCACCTCCACCGTTTCATAGCCAGCCGGTGGATATTTGCGATTATCGGAATTCGCCTCATCCTTCTCGTCCTCGTCACTGCTGGAGGCGGCGGAGGATGACTCCTCGTCGTCGGTTTGCTCTAGCCAAGGAGGCAGACAATTGGCCAAACGGCGAATGCAATTCCCAGGTAGGACATTCCTCTGGAAATTGAAATCGCGATCAAATCGCAGCTCCTCCAGCGGTCGCGGAGTATTCAACCTGGCCAAGTAGACATAATCGTAGTCCCCCGAACTGTAGCCCGAGGATGGGGAGTTGGATGCGGAGGATCCCACATCACCGTAGATGCATTCCTCATCGCTGCTGGCCGGCAAAGATAAGCTGGAGCTAGTGGGCGTGCCCGCCTGCGGATGCGGCAATTGGTCGCGCAGCTTGAGGCGAACATAGCCCATCCACCTGGTCATCTCCATCAAGTTGACTAGCTCCGAGGGGCAGGGCGTGGTCCGGTAGTAGTCCGTCTCCAGGAGCACCTTGCACAGTGCCGCCACGGTGTCGTAGTTGGCATCGTGGGGTCGCGCCACGTCAACGTTCTCAAAGACCACATAGGTCACGGGTTTCTGCAGCAGCTGTGGTGGACTCTTCGAA

 

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