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CRIMIC CR01535

Gene Tsp5D

CG4690

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:6069131..6074857	5D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTCGGACGATGACAGCTCAAGATA	GTTTCAATGAGCACGTGGAACTGG

Images

Construct Design Sequences

sgRNA sequence	CAGCTCGACGTAGACTTTGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAACCAGGAACTCGCTCAT	CGGCGTGTTTACCCTTAGCT	ACGTCGAGCTGGAAACTGAA	GACAAATGGACGGCCCTGA

 

Amplicon 5'

Amplicon 3'

CCAACCAGGAACTCGCTCATGAACAGCATTACAATCAGCATAAAGTACTGCGGGTAGATATGATATTGAATGGCTTTGCACAGAAAGAAAATGGTCAAAAAAAAGTACATAGATCACAAATTCGACATGCATGTATGCCATGAGCTAAATCCTCTCCGTTGACCCTGATTTACCCCCGATTTACTCACCAATACCAGGAGACAGCGGGATTGCACCCAGGCGCCGCAGCAGCCAAAGAAGCTGACCACAAATCCGGTGCCCCCGATGCCCATGAAGATGGTGTCCGCACTCAGACCTGCGTGCTGGGGCAGCAAAGTGGCGTATCCCGCGTAGCTCAGTCGCAACCAGAGTCCCGCTCCTAAAAACGCACAGCTACATAACTGCAATGGGTCAGGTTGGGAATTGGAAATTGGAAAATAGAAATTAGAAAGGCACTGATTATCCAGCAGAATGCTCGAAAAGTGAGAGGTGGTAGCTGCATACCTAAAGGACTCAAAAAGCTTAATATTTGATATAAATCAACGAGTCAATTATTCCAGACAGTGTCACCTGGACACAGACCTTGATTTATTGCTCTTTGCTGCACGAATAGCTGAAGGATCTATCATTCAGAGGCGAAATCAGATAATTCCCCCGATTGACGGCCAAATGGAGAGCTTTCGATTGCAAACTGTTGCATGTTCACAACGGCCTGACATCTGACATTTTCAGGGACTTCGGGGGAAACCCAGTGGCAAGCAAAAACAAAAGCGGACGGAAGCTGCAGCTGCAGCTCCAAAAAGCTAAGGGTAAACACGCCG

ACGTCGAGCTGGAAACTGAAACTGTGCTGAAAGCAAAGCCAAAAGCAAACGCAAAAGTCAATGACAGGCGCTCAGACAATGCAACATGCTGTCACAAAGACACTTCCGTCGCAGACGACGGCAAAGTTGTGGCTCTGATTAGCCGCCCTACGTACTGATTTTGATTTTGCCTGCGATTCTGATGCCGCGCATATCCGTTCTGGGGGAAATGGAGACGGCAGTCAGAATATATGTATCTTTCCAGAAATGTTTACTTATAGGCATAGAGGTGTGTATTTCAAGTCAACTTATTAGTAATTAGTAGGATTTTTTACTTAAATAACTTGCCCATAGAATGATGTTGAGCCAGCAGAATGTGCGGCGTATGCAAGTGTAGCCGGCATTGCCCATCGCGGGATCTGAAACGAAAAAAAAAACCATCAGGTAGAGCTCGAAACCTTTAATAGTCCTTTATATTTAGTGTAATTCCATCCTAAAAAGTATATCCTGTGGAAAAACCAGACCTTGCATCCATTAAACATCCATTTTGAAGCAGCAATTGCAATTCTGTTCGTTTATATATCAAATATAGTTTACTGTTATTATTATGTGTATTATTACTATGTTTTTTCATTGGCATTTCCTTTTGTCAGCGCCATGTAACTGGAGTACCCACATGTGTGTATGTGTGTGTGTGTGCCAGCGGGGGCGATTCGTTAGGCAATTGCAGCTGCAACTGCTGCACAAATGCAGTAATCCACAAATGTCCACACATGGGTAGACACTCGCTTATGCAAATATTTTGTATGATTGAATGAAAACAATAACTAAATAAAAAAAAATAACAAAATTAACAACAGCAGCGACCGCAATGACCATGGTCAACGAAGCAGATGAGCAAATAAATGGGTGGGTGTCCTCATCTGTATATTTAAACTATGGATGGCAAAAAGCATTCAGGGCCGTCCATTTGTC

 

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