• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01536

Gene CG7990

CG7990

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:19196058..19201979	18A7-18B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTGTGACCCTGCCAACAAAGCATA	ATGTGTCTCGTGGACATCGTCGTA

Images

Construct Design Sequences

sgRNA sequence	CGAAATGCGTGTATTTACACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTAGTGGATGGCAACCTGC	GGCGGCGCTTGTTTGTTT	AATTAGGCAAGCCAGCGAGA	AATAGCACCATCGACGGGAG

 

Amplicon 5'

Amplicon 3'

AGTAGTGGATGGCAACCTGCTGCTCCTGCGGCTCCCTCGCATCCGCCTGCTTGGAGCTAATCGTTGTGTTGATCTGCAAGGAGTTGCAAGGAATGGAGGCCATTAGGATCGAGCGGAGTTCAAGCAAAACCACTGCGCACTATACTTGGGATGTGCGAAATGCCACATAATAACATGTGATGGAGAGTATTTGCAACATATAGCTTTTCGTTATCAATTAAATGAATTAATTAATATTAAATTGATAGTTAAAATCCACTCATTGAGTTATTAACGTATAGGTAGTAATCAGTATTAGGGTTTTTCGAGTTATCATTGAAATTAAATATTTCCCAGGACAATGTTTGCTTATGTGGCATCGTTCCAGCCCAGAACCGCAGTGGTTTCGCCTCTTAAATGGAGGAGTGGAGAATGGAATGGGATGGCAGCCCTTACCTGGCCATAGTTGGTGGTCGAGTGTTTCGTTTTGGTTAATGCACAACAACGATAAAGCGCTAAAAAAAAAATAAATAAAGGTAAAAATAGAAATGAAAACCAAACAGAAGGCGTGTGAATAAATCAATAAACAAAAGACGAACGATGGGGCGCAGCGAATTAAGGCGGAAACTATGCGATCCGGCTGAATAGTGAATGCGGTTCGCCTGGCATCCCGAACTGGATGAGCCGGAGTTATAAATAAACAAGTCCTTGAGTTCTTGGGCAGACAAGGGGAGTAGGGGGCGGGGGCAGGATATGAATGATAGCCTGGGGGGGTGGCAAGGACACTCTCTCCCCCTTTCTCACTCTCTCTCTTGCTCTTCCTCTCTTTATCGTTCTCTGGCGCTGCGAAGCAGCAAAGGATTAAGGACCAAAAATAGACGAGCCTCGCCTGACGTAACCATTATTACCAACGGCAGCTGGCCAAAATTAATTAGCTCAAGTTAATGGGAAATTGGCCGGGCGTCAGAAATGAAATTAAATATGTGCTGCGATTGAATCGGGACACCGGAAGCGAGCAGCTCGAAGCTCGCCAAGAAACTGGGCCAAAGGTGGGTGGTTGGGTGGTTGGGTGGTTCGCAAGGACATGGCGAAATCGAATAAATGATTAAAACGAGAAACAAACAAACAAGCGCCGCC

AATTAGGCAAGCCAGCGAGAAAAACTAAATAAATGCGAAAACGTGTAGCAGAAATGCACGGAGAATATTTTTCATTAGCTCAATATTTTCCAAACTTCTAGAACTTAGCTGGGTTTACAAATCAAGTATTTGCAGTTCAAAAAGATTCATCCGTTATTTCATGCAGTTTACGATACATTTACAGGGATTCTCAAATATCAATTCTATTCTGTATGGCATTATATGTACTTATTGGTTATCCAATAAACTTTTGAGTTTCAGCAAGTTAGCCATTTGTGGAACACCTCTATTCCTGTAAGTGGTAAGCTTTCTTTCGGCTTTAAAGAGAGAATTGTGGTTTCGAAAGGAATATTATTAGCTTGATTACTTAGCCTTACGGAATAACTGGTGGAGTAAAAATATGTACTTGTGATTGTTTTTAAATCCACTGATTCTGTGTGCTAAATTTGGTTTCATTTTATTTTGTTGCAAAAATGGCTTTTAGCATTTCTGGATTTCTGGACTAATTTGAAAATATTTTCGGTGTGAAAAGTGTTGTGGGATGGTGGGAGGGGGTAGCAGGGGGGTGGCATGCGCGCTGACTGCGGAAAATTACAACAAACGAGCCGAGGAGGAGCATGGAAAATAAAACGTTCGCCCACACACACACACACATGTACACACGGACACACACAGTTGTTGTGCGCGTTTACAACGCTTTTTGCTGAGCAGCGGGGTGAAATGGGTGATGGTGGAGGATGATAAGGAGGAGGAGGGTTTGGGGGTGTCACTAAGCGTGCGCTGCCTCCGCTTAAAGTTATATAATTTTTGGCCAGGCCAAACAAACCATAAATACACACACTGGTTCGCACGAACACTTAAAAAAAAAAAAGAACAAGAAGAAAAAGTGGAAAGCAACAAAATTGGGCAACTCCATGGAAAAACGCCTCTCACCGCTGCTGGGTCGCTGGATCCGCCGGAGCAGCTGTTCCCGCCACCTCCCCCGCCGTCGCCAACGCACCTGGACACCAGGTCCGTCGCCCCCATCCCGCTATCTGCTGCGGATGACGTCGCCGCTCCGGTGCTCTGCTGCATTCTGTGCTCGGTTGTCGGCGTTGACGCTGGCGATGACTTTGGCTGTGGCGTTGCTGTTGCTGCTGCTGCTTCTCGTGCTCGTGCTCCCGTCGATGGTGCTATT

 

---

Search Again