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CRIMIC CR01539

Gene slpr

CG2272

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:8174716..8174721	7D17	FBti0210423	86437

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTCGGTGTCTTTCCCAAGGACTTT	GGTCACTGATTCACCCCAATGTCATC

Images

Construct Design Sequences

sgRNA sequence	GAACTGAAGGGCGCGGCGTAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGTCGCGTACAGCCGTAAT	GCGCCCTTCAGTTCTTCCAT	TAAGGCCAACAAGGAACGGT	ACTGGCTGGCAAGATACCAC

 

Amplicon 5'

Amplicon 3'

TTGTCGCGTACAGCCGTAATCGTGTGCCGGAAGCCCGTGGGCAGCGATATCTGCAGTGGTTTGTTCTAAAAGATAGATATAAGATATGTTATGCAAGATTTTTTTTTGGCTAATTTGACTCACATACCTTTTTGCCCTTTTTGTGTTTGCGTTTCGATGGCACCGGCTGCAACATGACAAGCTCCCGTTCGATCAACACTCTTTCACGCTCGCGCAGATTCTGCTCCCGGATCTTCAGGAGATTCGCCTTTTCGCGCTGCTCGTTTTGCACCCTGAGCAGCTGCTCTTCCTTGTTGCGCAGTTCCTGTCGGGATTAAAAGGGATAACATGGTGGAACGTGGCGGGTTAGTGTGGGCTAAGCATGATCACTGTACACGGGGACATGTGCTAGAAAATAGCATTAACTAGGAACTAGAAACCATGCAGCTGTTGGCTTGTGTGCGGCTCTATCGTGGGAGTTATCCAGCCAATAATAAAAAAAAAACAGGATAACTTTGGGTAAAATAGAATAGAGTAAAGTAATGTAGAATAGAGTAAAGTAATGTAACGAAACGAAACGAAACAACAGAACGAAACGCAACGCAACGCAATTGAAACGCAAACGGCGGGCAAGAGAAACCTGTTGGACGCGCTTACCTCTTCGATGGTTTGGAAACGCTTAACGTCGTTGGATGTTTTATTGTAAAACAAGGGAATTTTGTATTGTTTTGTTTTGGAGAGAGAGTATATACCAAATTATTAGAATTTCATCAACAACGACCAAGTGGGAGAAAGAGGGGAACGACTATGGAAGAACTGAAGGGCGC

TAAGGCCAACAAGGAACGGTCTAGAAACGATCTAGAGCAACTCAAACAGGTAGGGGGAGTTTATATTTTCAGTTTGCCTGGCGTTTTCCAGACAGTTGGTACCTTTTCTTTTTCGCGCAAATCGTGTAGCACTCCAGCGATCTCCTTTCGCCAGCATTCCTGCATGTAGTGAAACGACTCCTGGGGCGTTAGGGTGAATTTGGAGCATGCGATGCTCTCCAGCTGTTTGAGAATCTCCTTGAAGCCCGGTCGCTTGTGCGGATCCGTTTGCCAGCAGCCTGCGGGATTTGGACATAATGAAATATTACAATAATATGTATAGGATCATTGTCTATTGCGTTCACTTACTCTTCATCAGGGCTCCCCACGTCTCGGGGCAGGTTTTTGGTATGGGCAATGTCAGCGTGTTAACAGCCACGCCATAGGCCACGGACAACGGATCGAAGCCCTTGTAGGGCGTCTCGCCCGTTATCAGTTCCCACAGCAAGACACCGTAGCTCCAAACATCGGAGAATCTGGCGGGTAAGGAGTTTTGTTAGTTAGGAAGACAGACGACAGAGTGTGAGAGAAATGGAGCTCACTTGGAATAGGTGCTAACGCTGATGACCTCCGGTGGCATCCAAGCGTACGTGCCCGCCGCACTCATCCTCTGCGTGTTGTACATCTCCCTGGCCAAACCGAAATCCGTGATCTTGAGCGTCTTCTGCTGCAGATGATTGCCCTCGATGGCCTCGTAGATCAGCACATTGGAGGACTTGAGGTCGCGATGTATGATCGACATGGGCGCCTCATTGTGCAGATAGTTCATGCCTCTGGCTATCTGAATGGCCCAGTTGACTAGTACATCGGGTGGTATCTTGCCAGCCAGT

 

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